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Preparation and application of isoflavonoid compound

A technology of isoflavones and compounds, which is applied in the field of new isoflavones and their preparation, can solve the problems of unreported new compounds, etc., and achieve the effect of remarkable ability to scavenge free radicals, low cost, and wide sources

Active Publication Date: 2016-11-09
北京中京丰创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the new isoflavone compound found in mung bean plants is the structure of 2,3-epoxy-2′-hydroxydidainin, which is a new compound that has not been reported yet.

Method used

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  • Preparation and application of isoflavonoid compound
  • Preparation and application of isoflavonoid compound
  • Preparation and application of isoflavonoid compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the preparation of 2,3-epoxy-2'-hydroxydatinin

[0046] 1. Plant material: dried stems and branches of leguminous mung bean (Vigna radiate L.).

[0047]2. Separation and preparation method: dry 25 kg of mung bean plant stems, flash extract twice with 10 or 10 times the amount of 95% ethanol, filter, combine the filtrates, recover the ethanol under reduced pressure until there is no alcohol smell, and obtain about 5 L of concentrated solution; Use 5L of petroleum ether, 5L of dichloromethane, and 5L of n-butanol to extract 3 times each, combine the n-butanol extracts, recover n-butanol under reduced pressure, and obtain the mung bean plant extract; disperse the mung bean plant extract with an appropriate amount of methanol, And take an appropriate amount of silica gel to mix the sample; put the mixed sample into a silica gel column, and use dichloromethane-methanol (100:0~0:100) gradient elution to obtain 6 fractions, take fraction 4 [dichloro Methane-meth...

Embodiment 2

[0048] Example 2, In Vitro Antioxidative Activity Test of 2,3-epoxy-2'-hydroxydatinin

[0049] 1. Instruments: U-1900 spectrophotometer (Hitachi), AB135-S electronic balance (Mettler Toledo), microplate reader (BIO-RAD), pH-2TC (0.01 level) precision digital acidity meter (Shanghai Xin Instrument Co., Ltd.).

[0050] 2. Reagents: sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH 2 PO 4 ), dipotassium hydrogen phosphate (K 2 HPO 4 ), potassium persulfate (K 2 S 2 o 8 ), 2,2'-azino-bis-(3-ethylbenzodihydrothiazoline-6-sulfonic acid) diammonium salt (ABTS), ascorbic acid.

[0051] The 2,3-epoxy-2'-hydroxydaidin used in the experiment was self-made by the inventor, and its chemical structure was confirmed by hydrogen spectrum, carbon spectrum and mass spectrum, and its HPLC purity was above 98%.

[0052] 3. Experimental method: Prepare 7mmol / L ABTS solution and 2.45mmol / L potassium persulfate solution with phosphate buffered saline soluti...

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Abstract

The invention specifically relates to a preparation method and novel application of a novel isoflavonoid compound, belonging to the fields of medicine and chemistry of natural products. The isoflavonoid compound provided by the invention has a general structural formula as described in the specification. In the general structural formula, R1 and R2 have one to five glycosyl groups and may be H, (CH2)nCH3, or OR' (wherein R' may be H or (CH2)nCH3 and has one to five glycosyl groups); R3 and R4 may be H, (CH2)nCH3, or OR' (wherein R' may be H or (CH2)nCH3 and has one to five glycosyl groups); the glycosyl group is a glucosyl group or apiosyl group; and n is in a range of 0 to 3. The isoflavonoid compound provided by the invention has substantial antioxidation activity.

Description

technical field [0001] The invention belongs to the field of medicine and natural product chemistry, and specifically relates to a novel isoflavone compound, a preparation method thereof and a medical application. Background technique [0002] Mung bean is the dry mature seed of the leguminous plant mung bean (Vigna radiate L.), which has the effects of clearing heat, detoxifying and drying dampness. It can be used for the treatment of vomiting and diarrhea, macule, furunculosis, scabies and other diseases. "Materia Medica Huiyan" records that mung beans are used to treat furunculosis, eczema, metal, stone, dan, fire poisons and cholera vomiting. At present, there are no relevant reports on the pharmacological activity and active substances of mung bean plants. [0003] Isoflavones are ubiquitous in the plant kingdom, but epoxy isoflavones are rarely reported. At present, the new isoflavone compound found in mung bean plants is 2,3-epoxy-2'-hydroxydaidin, which is a new c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K31/352A61P39/06A61P9/00A61P25/28A61P35/00
CPCC07D493/04
Inventor 于治国赵云丽白岩
Owner 北京中京丰创科技有限公司
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