Method for extracting blueberry anthocyanin

A blueberry anthocyanin and extraction method technology, applied in the field of blueberry anthocyanin extraction, can solve the problems of increasing the dissolution rate of blueberry anthocyanin, difficulty in industrial production, expensive equipment, etc., achieve high medical care value, promote dissolution, The effect of wide practical application field

Inactive Publication Date: 2016-11-09
仇颖莹
View PDF3 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: In the process of extracting anthocyanins in the current traditional method, water extraction or solvent extraction will destroy the anthocyanin structure due to high temperature, affecting its biological activity and yield, while supercritical extraction Although the method can improve the extraction rate, the equipment is expensive, the cost is high, and the status quo that is not easy for industrialized production provides a kind of blueberry fruit as raw material, crushing and beating, using a mass fraction of 40% ethanol solution as the extraction solution, and adding cellulase , extracted with the assistance of ultrasound, and then centrifuged, precipitated and leached repeatedly once, and after the combined extract was concentrated, a short pulse of high voltage was applied to the material between the two electrodes through a high-voltage pulse electric field, so that the polar substances in the action of the electric field Move towards the electrode at a high speed, and use the principle of cell membrane electroporation to cause irreversible damage to the tissue cells, thereby promoting the dissolution of blueberry anthocyanins, increasing the dissolution of blueberry anthocyanins in the extract, and then adsorbed by macroporous adsorption resin and washed with alcohol , concentrated, freeze-dried, thereby obtaining blueberry anthocyanin extraction method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] First weigh 200g of blueberries, wash and remove impurities, add 200mL of deionized water to a homogenizer for crushing and beating for 10 minutes, add the blueberry slurry to 2L of 40% ethanol solution, and add 30g of cellulose in a constant temperature water bath at 50°C Enzyme, and use 200W ultrasonic wave for 10min, adjust the pH to 2.0 with a mass concentration of 10% hydrochloric acid solution, extract for 2h, put the extract into a 4000 / min centrifuge and centrifuge for 10min to obtain the precipitate and supernatant, and press the precipitate The above operation was leached again and the supernatant was collected, and the supernatant was combined, and the supernatant was concentrated under reduced pressure to 30% of the original volume at 50°C to obtain a crude extract of blueberry anthocyanins; then the above blueberry flower The crude extract of cyanidin was added to a 60% ethanol solution containing 0.1% hydrochloric acid at a material-to-liquid ratio of 1:6, ...

example 2

[0021] First weigh 250g of blueberries, wash and remove impurities, add 250mL of deionized water to a homogenizer for crushing and beating for 13 minutes, add the blueberry slurry to 2.5L of 40% ethanol solution, and add 40g of fiber in a constant temperature water bath at 51°C and use 200W ultrasonic wave for 11min, adjust the pH to 2.3 with a mass concentration of 10% hydrochloric acid solution, extract for 2.5h, put the extract into a 5000 / min centrifuge and centrifuge for 13min to obtain a precipitate and supernatant, and The precipitate was leached once more according to the above operation and the supernatant was collected, and the supernatant was combined, and the supernatant was concentrated under reduced pressure at 53°C to 33% of the original volume to obtain a crude blueberry anthocyanin extract; then the above The crude blueberry anthocyanin extract was added to a 60% ethanol solution with a mass concentration of 0.1% hydrochloric acid at a material-to-liquid ratio ...

example 3

[0024]First, weigh 300g of blueberries, wash and remove impurities, add 300mL of deionized water to a homogenizer to crush and beat for 15 minutes, add the blueberry slurry to 3L of 40% ethanol solution, and add 45g of cellulose in a constant temperature water bath at 52°C Enzyme, and use 200W ultrasonic wave for 12min, adjust the pH to 2.5 with a mass concentration of 10% hydrochloric acid solution, extract for 3h, put the extract in a centrifuge at 6000 / min for 15min, obtain the precipitate and supernatant, and press the precipitate The above operation was leached again and the supernatant was collected, and the supernatant was combined, and the supernatant was concentrated under reduced pressure to 35% of the original volume at 55°C to obtain a crude extract of blueberry anthocyanins; then the above blueberry flower The crude extract of cyanidin was added to a 60% ethanol solution containing 0.1% hydrochloric acid at a material-to-liquid ratio of 1:7, and passed through a hi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting blueberry anthocyanin, and belongs to the field of natural organic chemistry. Blueberry fruits are adopted as raw materials, crushed and pulped, an ethanol solution with the mass fraction being 40% is adopted as an extraction solution, cellulase is added, and extraction is carried out under ultrasonic assistance; centrifugal separation is carried out, precipitation and repeated digestion are carried out once, extraction solutions are combined and concentrated, then the blueberry anthocyanin is promoted to be dissolved out through a high-voltage pulse electric field, and the dissolving-out amount of the blueberry anthocyanin of the extraction solutions is increased; then macroreticular resin adsorption, alcohol washing, concentration and freeze drying are carried out, and therefore the method for extracting the blueberry anthocyanin is obtained. A living example proves that operation is controllable, the method is unique, the production cost is reduced, pollution is avoided, the extraction rate of the prepared blueberry anthocyanin is as high as 80% or above, the purity reaches 75% or above, and higher medical care value and a wider practical application field are achieved.

Description

technical field [0001] The invention discloses a method for extracting blueberry anthocyanins, belonging to the field of natural organic chemistry. Background technique [0002] Anthocyanins, also known as anthocyanins, are a class of water-soluble natural pigments that widely exist in plants. Due to the instability of anthocyanins, anthocyanins mainly exist in plants. [0003] As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have certain nutritional and pharmacological effects. They have great application potential in food, cosmetics, and medicine. Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have proved that it is the most effective antioxidant discovered by humans today, and it is the most effective antioxidant substance in nature. At the same time, studies have claimed that it can enhance vision, eliminate eye fatigue, and delay the aging of brain nerves. The anthocyanins contained in blueberries are curr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D311/62C09B61/00
CPCC07D311/62C09B61/00
Inventor 仇颖莹张明王龙
Owner 仇颖莹
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap