Preparation method of compound nanoparticles for reducing aflatoxin absorption and liver damage

A technology of aflatoxin and absorbability, applied in the field of preparation of compound nanoparticles, can solve the problem of reducing the bioavailability of aflatoxin

Active Publication Date: 2018-12-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

From the perspective of new catechin and proanthocyanidin compound nano-solution and reducing the bioavailability of aflatoxin to study the protective function of aflatoxin, there is no report yet

Method used

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  • Preparation method of compound nanoparticles for reducing aflatoxin absorption and liver damage
  • Preparation method of compound nanoparticles for reducing aflatoxin absorption and liver damage
  • Preparation method of compound nanoparticles for reducing aflatoxin absorption and liver damage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1, a kind of preparation method of the compound nanoparticle that reduces intestinal absorption of aflatoxin and liver injury, carries out the following steps successively:

[0069] 1), accurately weigh 12g of catechins and proanthocyanidins mixed in equal amounts (that is, the mass ratio of catechins and proanthocyanidins is 1:1), add 200mL of sodium polyphosphate solution with a mass concentration of 1.5%, well mixed;

[0070] 2), add water to 1000ml in 5g of chitosan, in 5000mL round bottom flask, after magnetic stirring (speed 1000r / min), adjust pH 4.0, obtain chitosan liquid;

[0071] 3), the solution prepared in step 1) (that is, the sodium polyphosphate solution of catechins and proanthocyanidins) was uniformly and slowly added dropwise to the round bottom flask in 2), and the addition was completed in 70 minutes (ie , the dropping rate is about 50 drops / minute);

[0072]The obtained reaction system was continuously stirred at 25° C. for 60 minutes (...

Embodiment 2

[0073] Embodiment 2, a kind of preparation method of the compound nanoparticle that reduces intestinal absorption of aflatoxin and liver damage:

[0074] Change the mass ratio of catechins and proanthocyanidins in step 1) from 1:1 to 1:3; the total weight remains unchanged, still 12g;

[0075] Change the consumption of chitosan into 2g by 5g in step 2);

[0076] The rest are equal to Example 1.

Embodiment 3

[0077] Embodiment 3, a kind of preparation method of the compound nanoparticle that reduces intestinal absorption of aflatoxin and liver damage:

[0078] Change the mass ratio of catechins and proanthocyanidins in step 1) from 1:1 to 3:1; the total weight remains unchanged, still 12g;

[0079] Change the consumption of chitosan in step 2) into 1.5g by 5g;

[0080] The rest are equal to Example 1.

[0081] Experiment 1. Detection of encapsulation efficiency of nanoparticles:

[0082] The compound nanoparticle suspension of embodiment 1, embodiment 2, embodiment 3 gained is detected according to the above method, and the results are as follows:

[0083] The encapsulation efficiency (%) of the compound nanoparticle suspension obtained in Example 1=75.57%;

[0084] The encapsulation efficiency (%)=53.84% of the compound nanoparticle suspension obtained in embodiment 2;

[0085] The encapsulation efficiency (%) of the compound nanoparticle suspension obtained in Example 3=41.94...

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Abstract

The invention discloses a preparation method of compound nanoparticles for reducing aflatoxin absorbance and liver injury. The preparation method comprises the following steps: 1) firstly, mixing catechin and procyanidine according to an equivalent mass, so that a mixed material is obtained; adding 200ml of a sodium polyphosphate solution which is 1.4-1.6% in mass concentration to 12g of the mixed material, and uniformly mixing the materials; 2) adding water to 4-6g of chitosan, setting a constant volume to 1000ml, conducting magnetic stirring and regulating pH value to 3.5-4.5, so that chitosan liquid is obtained; and 3) completely adding the solution obtained in the step 1) to the chitosan liquid obtained in the step 2), and continuing to stir an obtained reaction system at 20-30 DEG C for 40-80min, so that a compound nanoparticle suspension is obtained.

Description

technical field [0001] The invention relates to a preparation method of compound nano-particles for reducing intestinal absorption and liver damage of aflatoxin. Background technique [0002] Tea Polyphenols (Tea Polyphenols, TP) is the general term for polyphenols and their derivatives in tea, mainly composed of catechins, flavonoids, anthocyanins and phenolic acids, among which catechins account for the majority of tea polyphenols. Total 60%-90%. Catechin is the most important natural active ingredient in tea, which has a variety of pharmacological and health-care activities; catechin has strong biological activity, and a large number of studies have shown that catechin has antioxidant, free radical, anti-cardiovascular Various health care and pharmacological effects such as antibacterial and anti-inflammatory. Proanthocyanidin is a natural super antioxidant that exists in plants such as grape seeds, black wolfberry, ginkgo biloba, pine bark, and roses. It has become the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61K9/16A61K9/10A61P1/16A61P39/02A61K31/352
CPCA61K9/10A61K9/1652A61K31/352A61K31/353A61K2300/00
Inventor 肖英平杨华吉小凤钱鸣蓉李锐王小骊吴俐勤
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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