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Myricaria germanica effective part, preparation method and use thereof

A technology of effective parts and water cypress, applied in the field of medicine, can solve the problems of unclear active ingredients, no research reports and patents, etc.

Inactive Publication Date: 2016-11-02
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above research reports have confirmed the activity of Cypress branch in the treatment of arthritis, the active part is only the crude extract, and the active ingredients are not clear. Based on the previous research work, this patent has further confirmed the The effective parts and active ingredients in the treatment of arthritis, so far, no research reports and patents have been seen in this area

Method used

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  • Myricaria germanica effective part, preparation method and use thereof
  • Myricaria germanica effective part, preparation method and use thereof
  • Myricaria germanica effective part, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: the preparation of the effective part of Shuibai branch

[0058] Select 15 kg of dry twigs of Myricaria bracteata, heat and reflux extraction with 6 times the amount of ethanol for 3 times, each time for 2 hours, recover the ethanol from the extract under reduced pressure and concentrate to obtain an extract. Add appropriate amount of water to disperse and dissolve the extract, and extract with petroleum ether and ethyl acetate in sequence to obtain the petroleum ether fraction, ethyl acetate fraction and water fraction respectively. The water part is filtered, and the filtrate is separated by the treated macroporous adsorption resin HP-20, and is eluted with 8 times the amount of water, 30% ethanol, 50% ethanol and 95% ethanol respectively, and the eluted part of 30% ethanol is recovered under reduced pressure. After freeze-drying, 141.1 g of the effective fraction was obtained. (See figure 1 )

Embodiment 2

[0059] Example 2: Component Analysis of the Effective Parts of Shuiba Branch

[0060] The active ingredient in the effective part of Cypress branch is a hydrolyzable tannin compound, which is formed by linking glucose and gallic acid with an ester bond or a glycoside bond.

[0061] The ultraviolet (UV) spectrum of hydrolyzable tannin compounds generally shows two UV characteristic absorption peaks in the ranges of 205-224 and 260-283 nm. (See figure 2 )

[0062] The detection method of the hydrolyzable tannin compounds of the effective components in the effective parts of Shuibaizhi adopts high performance liquid chromatography. The HPLC determination method adopts the binary high-pressure gradient elution method, and the analytical chromatographic column is COSMOSIL 5C 18 -PAQ Waters (4.6i.d.×250mm), the mobile phase is A (water containing 2% acetic acid) and B (acetonitrile), the elution gradient is 0-1min, 5% B, 1-5min, 5%-15% B, 5-40 min, 15%-20% B, the column tempera...

experiment example 1

[0070] Experimental example 1: The test of the effective part of Shuibaizhi (SBZ group) inhibiting ear swelling of mice induced by croton oil.

[0071] Kunming mice were randomly divided into model group, blank control group and SBZ group, with 7 mice in each group. Each administration group was intragastrically (ig) administered with the corresponding drug once, with an administration dose of 100 mg / kg, and the model group was administered an equal volume of 0.1% polysorbate-80 solution. After 1 hour, mice in each group were evenly smeared with 2% croton oil on both sides of their left ears for sensitization. After 4 hours of sensitization, they were killed. The left and right auricles were cut off, and ear pieces of the same size were made with a stainless steel puncher with a diameter of 8 mm, and the weights were weighed respectively. , taking the difference between the two ears as the swelling degree.

[0072] The test results of croton oil-induced ear swelling in mice a...

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Abstract

The invention relates to a Myricaria germanica effective part, a preparation method and use thereof in treatment of rheumatism and rheumatoid arthritis. Animal experiments prove that the effective part can inhibit croton oil induced mouse ear swelling and rat adjuvant or collagen induced arthritis, and can be used for prevention and / or treatment of rheumatism and rheumatoid arthritis.

Description

technical field [0001] The present invention relates to the effective part extracted from the traditional Tibetan medicine Shuibaizhi, including the preparation method of the effective part, the pharmaceutical composition containing the effective part, and the effective part containing the effective part and its pharmaceutical composition in the treatment of rheumatism and rheumatoid The application in the medicine of arthritis, the invention belongs to the technical field of medicine. Background technique [0002] Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint synovitis, often accompanied by inflammatory cells (T cells, neutrophils, macrophages, etc.) Hyperplasia, hyperplasia of joint synoviocytes, thickening of the subsynovial synovium to form villous protrusions, and bone and cartilage damage and other pathological changes. This inflammation can deform the joints to the point of disabling them, and some loss of mobility due to joint pain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P29/00A61P19/02A61P37/02A61K31/7024A61K31/192
CPCA61K31/192A61K31/7024A61K36/185A61K2236/33A61K2236/331A61K2236/333A61K2236/55
Inventor 李帅侯琦刘佳宝袁绍鹏白金叶张瑛
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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