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Device based on micro-nano fluid control reflection interference spectral imaging system and realization method

A technology of reflection interference and spectral imaging, which is applied in the directions of using multiple reflections to generate spectra, spectrum generation, and measurement of phase-influenced characteristics. It can solve the problems of inability to accurately locate the test position and shape characteristics of tiny samples, and achieve convenient and accurate detection processes. High and improve the effect of integration

Inactive Publication Date: 2016-10-26
NORTHWEST UNIV(CN)
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Problems solved by technology

This method uses an optical fiber probe to detect the sample to obtain the interference spectrum signal, which cannot accurately locate the test position and shape characteristics of the tiny sample.

Method used

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  • Device based on micro-nano fluid control reflection interference spectral imaging system and realization method
  • Device based on micro-nano fluid control reflection interference spectral imaging system and realization method
  • Device based on micro-nano fluid control reflection interference spectral imaging system and realization method

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Embodiment

[0052] Embodiments of devices and implementation methods based on micro-nanofluidic reflection interference spectroscopy imaging systems are given below.

[0053] In this embodiment, the device based on the micro-nanofluidic reflection interference spectroscopy imaging system includes a microfluidic pump 1, a microfluidic sample cell system 2, an optical microscope 4, a C-port optical path switcher 5, a CCD sensor 6, a computer 7, and a spectrometer 8. Locating the light source 9 and detecting the light source.

[0054] Microfluidic pump with a flow rate range of 0.004ml / min; the microfluidic sample pool system 2 is a micro-nano structure, the microstructure is 25μmx10μmx15μm, the diameter of the nanostructure is 50nm, and the thickness is 8um; the magnification of the objective lens 3 of the optical microscope 4 is 10 times , numerical aperture 0.70; the detection light source of the optical microscope 4 is a uniform light source halogen lamp, the power is 100W, and the wave ...

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Abstract

The invention discloses a device based on a micro-nano fluid control reflection interference spectral imaging system and a realization method. The device comprises a micro fluid pump, a micro fluid control sample cell system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection light source, and a computer. The C-port optical path switcher is connected to the positioning light source, the CCD sensor, and the spectrometer through optical fibers; the CCD sensor and the spectrometer are both connected to the computer, and the computer software collects detection data and analyzes the results. According to the system, the object lens of the microscope is taken as the optical probe; compared with the conventional method that takes a detector as the probe, the system has the advantages that a fixing device for fixing a probe is not needed, the optical path integration degree of the system is improved; moreover, an imaging and positioning system is used to obtain the specific position of a sample, and thus the detection precision is improved. The morphology feature imaging of samples and reflection interference spectrum collection can be switched in real time, and thus the in-situ dynamic realtime detection of an analysis sample can be realized.

Description

technical field [0001] The invention relates to a device and a realization method based on a micro-nano fluidic reflection interference spectrum imaging system. Background technique [0002] A detection method based on micro-nanofluidic reflection interference spectroscopy imaging system is convenient, high-precision, non-contact and non-destructive detection of samples, and can directly observe and collect the surface morphology and reflection interference signals of trace samples. Among them, reflection interference spectroscopy does not require a coherent light source, which makes it simple in structure and stronger in anti-interference ability, and can be well applied to the detection of trace samples. Reflection interference spectroscopy imaging technology has more practical significance and practical value. For example, in the field of microbiology, the reflectance interference spectroscopy imaging system studies the type and quantity of microorganisms by detecting the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/45G01J3/26
CPCG01J3/26G01N21/45
Inventor 王凯歌武青青孙聃张琛王爽赵伟冯晓强白晋涛
Owner NORTHWEST UNIV(CN)
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