Biophoton spectrum detection system and method
A biophotonic and spectral detection technology, applied in the field of biological and biomedical technology imaging, can solve the problems of insufficient attention, weak biophoton intensity, and no biophoton detection methods, and achieve rich functions, diverse choices, and good quality. The effect of scalability and upgrade potential
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[0037] Example: Glutamate-induced biophotonic spectroscopy in mouse brain slices
[0038] 1. Test materials: adult Kunming mice, from the Public Service Center for Experimental Animals of Hubei Province.
[0039] 2. Detection steps:
[0040] (1) Test preparation: Before starting the test, first turn on the air conditioner to keep the laboratory room temperature at about 25°C, then use ultrapure water to clean the brain slice perfusion system, and turn on the EM-CCD and low-temperature coolant circulation pump to make the The EM-CCD is preheated in a working environment of -95°C.
[0041] (2) Laser calibration: Three lasers with different wavelengths of 405nm, 532nm and 650nm are used to calibrate the biophoton detection system. Moreover, the laser light source needs to be calibrated at normal intensity and biophoton intensity respectively.
[0042] The calibration results are attached Figure 2a As shown, the upper figure is the laser spectrum at normal intensity, and the ...
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