A method for group division of Chinese giant salamander based on mitochondrial DNA sequence

Abstract

The invention discloses a Chinese giant salamander group dividing method based on a mitochondrial DNA sequence. The method includes: (1), collecting a giant salamander sample, and extracting genome DNA; (2), synthesizing a giant salamander specific mitochondrial DNA primer; (3), performing PCR (polymerase chain reaction) amplification on the giant salamander sample, and sequencing PCR amplification products; (4), comparing an acquired sequence with a reference sequence; (5), according to sequence features of corresponding sites, judging a group to which the sample belongs. The giant salamander mitochondrial DNA sequence is used as a sequence marker for differentiating Chinese giant salamander groups, and target groups can be identified quickly and accurately by comparing the giant salamander mitochondrial DNA sequence with sequence features of the reference sequence. The method can be used for identifying giant salamander individuals in giant salamander farms to determine origin of the individuals; the method can assist in breeding selection during parental breeding. The method can also be applied in identifying multiplication and releasing samples, determining groups to which the samples belong and then releasing and the samples to proper regions, so that germplasm hybridization of wild groups by released groups is avoided.

Description

technical field [0001] The invention belongs to the technical field of biological identification, and in particular relates to a method for grouping Chinese giant salamanders based on mitochondrial DNA sequences. Background technique [0002] The Chinese Giant Salamander (Andrias davidianus) is the largest amphibian among the existing amphibians. The largest individual can reach a length of more than 1 meter and a weight of more than 8 kilograms. The Chinese giant salamander is only distributed in my country and is endemic to my country. The distribution areas include Beijing, Hebei, Henan, Shanxi, Shaanxi, Gansu, Qinghai, Sichuan, Guizhou, Hubei, Hunan, Anhui, Jiangsu, Zhejiang, Jiangxi, Fujian, Guangdong and Guangxi, etc. Province City District. Due to its important scientific research and economic value, the market demand is very large, leading to overfishing of giant salamanders in China, coupled with the reduction of habitats, the current wild resources of giant salaman...

AI Technical Summary

Problems solved by technology

[0004] At present, some workers identify giant salamanders of different geographical groups by their body surface patterns, but the body surface patterns are not stable and have no fixed signs, so it is difficult to use them as a standard for group division

There are some reports at home and abroad that use mitochondrial DNA to analyze a small number of samples and find that there are certain genetic differences in Chinese giant salamander populations, but no clear population markers have been proposed

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