Ultrasonic-cavitation-injury-based method for establishing atherosclerotic-plaque animal model and blood vessel endothelial injury device

A technology of atherosclerosis and animal models, which is applied in the field of establishing experimental animal disease models, which can solve problems such as increasing the injection dose, reducing the success rate of modeling, and not being easy to model AS plaques, achieving the effect of reducing difficulty and complexity

Active Publication Date: 2016-10-26
XI AN JIAOTONG UNIV
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they also have shortcomings. First, the large area of ​​endothelial damage makes it difficult to accurately predict the exact location and size of the formed plaque.
Because the location of plaque formation is uncertain, in the process of model construction, the evaluation of plaque formation and plaque development process can only be evaluated by non-living pathological anatomical analysis methods, so the sample size of model culture is large and the operation is complicated. , it is difficult to track the development process of a certain plaque, so it is not easy to obtain AS plaque models at different development stages for testing the sensitivity and effectiveness of ima

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ultrasonic-cavitation-injury-based method for establishing atherosclerotic-plaque animal model and blood vessel endothelial injury device
  • Ultrasonic-cavitation-injury-based method for establishing atherosclerotic-plaque animal model and blood vessel endothelial injury device
  • Ultrasonic-cavitation-injury-based method for establishing atherosclerotic-plaque animal model and blood vessel endothelial injury device

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0048] Instruments and contrast agents:

[0049] FU transducer parameters: single array element FU transducer, center frequency 1.2MHz, focal length 120mm, outer diameter 156mm, middle hole diameter 50mm, acoustic-electric conversion efficiency 72%, half-height width 8mm*1.2mm;

[0050] B-ultrasound imaging probe: Anke AUS-3500 phased array;

[0051] Full digital color ultrasonic diagnostic instrument: Anke AUS-3500;

[0052] Multi-dimensional programmable mechanical cantilever: electric three-dimensional mobile platform, including three-dimensional mobile mechanical axis, cantilever and spherical joint. The cantilever is fixed on the mechanical shaft, the spherical joint is fixed on the cantilever, and the probe fixing device 4 is fixed on the spherical joint; the probe fixing device 4 is made of plexiglass and is used to fix the composite probe;

[0053] Power amplifier: AG1016, T&C Power Conversion, USA;

[0054] Arbitrary waveform generator: AWG2021, Sony / Tektronix, JP;...

example 2

[0067] Instruments and contrast agents:

[0068] FU transducer parameters are: 1.1 / 5MHz dual-frequency ring array transducer, outer ring 1.1MHz, inner ring 5MHz, focal length 60mm, outer diameter 103mm, middle hole diameter 25mm;

[0069] B-ultrasound imaging probe: Ultrosonix PA4-2 / 20;

[0070] Full digital color ultrasonic diagnostic instrument: Ultrosonix RP;

[0071] Other equipment is as described in Example 1;

[0072] Contrast agent: microbubble contrast agent.

[0073] Damage implementation steps:

[0074] (1) Anesthetize the rabbit with a respiratory anesthesia machine, prepare the skin on the abdomen, and fix the rabbit on the operating table with the abdomen facing upward.

[0075](2) Observe the position and direction of the abdominal aorta to be damaged in the B-ultrasound image, and make the focus of the focused ultrasound fall on the artery to be damaged according to the position and direction.

[0076] (3) Set the path and moving step of the electric three...

example 3

[0080] Instruments and contrast agents:

[0081] Multi-dimensional programmable mechanical cantilever: six-axis robotic arm

[0082] Other equipment is as described in Example 1.

[0083] Contrast agent: microbubble contrast agent.

[0084] Damage implementation steps:

[0085] (1) Anesthetize the rabbit with a respiratory anesthesia machine, prepare the skin on the abdomen, and fix the rabbit on the operating table with the abdomen facing upward.

[0086] (2) Observe the position and direction of the abdominal aorta to be damaged in the B-ultrasound image, and make the focus of the focused ultrasound fall on the artery to be damaged according to the position and direction.

[0087] (3) Set the moving path and moving step of the six-axis manipulator according to the requirements of the direction of the abdominal aorta and the injury area (for example: move 1cm along the direction of the blood vessel, and the step length is 2mm), and load the timing control signal of the arb...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Center frequencyaaaaaaaaaa
Focal lengthaaaaaaaaaa
Outer diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses an ultrasonic-cavitation-injury-based method for establishing an atherosclerotic-plaque animal model and a blood vessel endothelial injury device. The device comprises a composite probe, a B-mode image guiding module, a motion control module, a time sequence signal control and power drive module and a cavitation detection module. According to the invention, an endothelial ultrasonic-cavitation injury of a local artery blood vessel with controlled injury site, injured area, and injury degree can be realized; and then high-fat diet feeding is combined to form a method for establishing an atherosclerotic-plaque animal model with controllable and predictable plaque formation position and size. An atherosclerotic-plaque animal model for detecting imaging method sensitivity and effectiveness at different development stages can be established; no surgical operation is needed during the artery blood vessel endothelium injuring process; and the blood vessel endothelial injury difficulty and complexity are reduced.

Description

technical field [0001] The invention belongs to the category of establishing experimental animal disease models, and relates to a method for establishing an animal model of atherosclerotic plaque based on ultrasonic cavitation damage and an arterial vessel endothelial injury device system. Background technique [0002] Atherosclerosis (AS) refers to the appearance of yellow substances such as cholesterol and fat in the intima of large and medium arteries, forming yellow plaques like atheroma, accompanied by smooth muscle cells and macrophages under the intima Proliferation, causing pathological changes in arterial wall thickening and hardening, often leading to thrombosis and lumen stenosis. It is not only a disease of the artery itself, but also the main cause of myocardial infarction, cerebral infarction, gangrene and loss of limb function, and the main pathological basis of cardiovascular and cerebrovascular events. In developed countries, AS cardiovascular disease is kn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61N7/02A61D7/00A61D1/00A23K20/168A23K20/158A23K20/147
CPCA61N7/022A61D1/00A61D7/00
Inventor 宗瑜瑾刘刚万明习张蕾
Owner XI AN JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap