Ultrasonic-cavitation-injury-based method for establishing atherosclerotic-plaque animal model and blood vessel endothelial injury device

Abstract

The invention discloses an ultrasonic-cavitation-injury-based method for establishing an atherosclerotic-plaque animal model and a blood vessel endothelial injury device. The device comprises a composite probe, a B-mode image guiding module, a motion control module, a time sequence signal control and power drive module and a cavitation detection module. According to the invention, an endothelial ultrasonic-cavitation injury of a local artery blood vessel with controlled injury site, injured area, and injury degree can be realized; and then high-fat diet feeding is combined to form a method for establishing an atherosclerotic-plaque animal model with controllable and predictable plaque formation position and size. An atherosclerotic-plaque animal model for detecting imaging method sensitivity and effectiveness at different development stages can be established; no surgical operation is needed during the artery blood vessel endothelium injuring process; and the blood vessel endothelial injury difficulty and complexity are reduced.

Description

technical field [0001] The invention belongs to the category of establishing experimental animal disease models, and relates to a method for establishing an animal model of atherosclerotic plaque based on ultrasonic cavitation damage and an arterial vessel endothelial injury device system. Background technique [0002] Atherosclerosis (AS) refers to the appearance of yellow substances such as cholesterol and fat in the intima of large and medium arteries, forming yellow plaques like atheroma, accompanied by smooth muscle cells and macrophages under the intima Proliferation, causing pathological changes in arterial wall thickening and hardening, often leading to thrombosis and lumen stenosis. It is not only a disease of the artery itself, but also the main cause of myocardial infarction, cerebral infarction, gangrene and loss of limb function, and the main pathological basis of cardiovascular and cerebrovascular events. In developed countries, AS cardiovascular disease is kn...

AI Technical Summary

Problems solved by technology

However, they also have shortcomings. First, the large area of ​​endothelial damage makes it difficult to accurately predict the exact location and size of the formed plaque.

Because the location of plaque formation is uncertain, in the process of model construction, the evaluation of plaque formation and plaque development process can only be evaluated by non-living pathological anatomical analysis methods, so the sample size of model culture is large and the operation is complicated. , it is difficult to track the development process of a certain plaque, so it is not easy to obtain AS plaque models at different development stages for testing the sensitivity and effectiveness of imaging methods, especially for small plaques, which cannot meet the growing Research needs of medical imaging methods

Second, the degree of endothelial damage is difficult to control, that is, poor control of the balloon size leads to arterial rupture or too mild damage, which reduces the success rate of modeling

Third, surgery is required during endothelial injury, which increases the difficulty and complexity of the modeling process

Usually, for ordinary microbubble contrast agents, in order to maintain a high local concentration, it is necessary to increase the injection dose, which may cause the attenuation of ultrasonic energy in the area to be damaged due to the "shielding" effect

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