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Method for determination of phytic acid content of cotton seed powder

A method of determination, cotton kernel powder technology, applied in the field of determination of phytic acid content of cotton kernel powder, can solve the problems of long analysis time, reagent toxicity, high detection cost, etc., achieve accurate spectral data, promote processing and utilization, and accurate determination method Effect

Inactive Publication Date: 2016-10-12
ZHEJIANG UNIV
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Problems solved by technology

However, these traditional methods have problems such as cumbersome sample preparation, high toxicity of reagents, long analysis time, low sensitivity and high detection cost.

Method used

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  • Method for determination of phytic acid content of cotton seed powder
  • Method for determination of phytic acid content of cotton seed powder
  • Method for determination of phytic acid content of cotton seed powder

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Experimental program
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Effect test

specific Embodiment

[0035] 1) Selection of samples

[0036] The samples are cottonseeds of different varieties grown in 10 different ecological regions across the country in 2014, including Hangzhou in Zhejiang, Wuhu in Anhui, Yancheng in Jiangsu, and Lixian in Hunan. There are a total of 280 cottonseed samples.

[0037] 2) Preparation of cotton kernel powder samples

[0038] After the cottonseed is delinted, dried, shelled and dried again, the cotton kernel is ground into powder with a sample mill, and passed through a 60-mesh sieve to obtain a cotton kernel powder sample;

[0039] 3) Sample Spectrum Collection

[0040] Each cotton kernel powder sample was loaded into the sample cell three times, and the spectral data was obtained after scanning, and the average spectral value of the three spectral data of each sample was calculated and converted into log(1 / R). Sample raw spectrogram, such as figure 2 shown.

[0041] Near-infrared spectrum collection conditions: use Büchi NIR Flex-N500 Fourie...

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Abstract

The invention discloses a method for determination of phytic acid content of cotton seed powder. The method comprises collecting different kinds of cottonseed samples planted in different regions, carrying out sample husking, grinding, sieving and moisture balance on the samples, collecting full spectrum data, pretreating the near infrared spectroscopy data through a plurality of pretreatment methods, accurately determining sample phytic acid content through high performance ion chromatography, constructing the optimal PLS model in a full spectrum range through a full cross-validated method, carrying out variable selection on the spectroscopic data, building multiple correction models through a multivariate calibration regression method, building a near infrared spectroscopy correction model and detecting phytic acid content of cotton seed powder through the model. The method utilizes a Buchi NIR Flex-N500 Fourier transform near infrared spectrometer to acquire a spectrogram of cotton seed powder, has a fast determination speed and high accuracy, is environmentally friendly, convenient and efficient and has an important meaning for cultivation of low-phytic acid cotton and promotion of cotton side product processing and utilization.

Description

technical field [0001] The invention discloses a method for determining the content of agricultural by-products, and in particular relates to a method for determining the content of phytic acid in cotton kernel powder. Background technique [0002] Cottonseed is the main by-product of cotton. The annual output of cottonseed is more than 10 million tons nationwide, and the annual output of cottonseed cake is more than 6 million tons. It is widely distributed and has the largest resource volume in the world. Cottonseed is rich in protein (27.83-45.60%) and fat (28.24-44.05%). Cottonseed oil and cottonseed cake are obtained by pressing after shelling. Cottonseed oil is edible, and cottonseed cake is used as poultry and livestock feed. The protein content in cottonseed cake is second only to soybean meal, and compared with rice and wheat, the protein content is 5-8 times higher. Cottonseed cake can be hydrolyzed to obtain 17 kinds of amino acids. In terms of essential amino ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N30/02
CPCG01N21/359G01N30/02G01N2021/3595
Inventor 祝水金赵茹冰陈进红李诚徐晓建胡佳慧
Owner ZHEJIANG UNIV
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