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A method for rapidly detecting the anti-inflammatory activity of plant water extracts

A technology of plant water and plant extract, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of complex components, different shades of color, unsuitable plant water extract, etc., and achieve the effect of stable results and low cost

Active Publication Date: 2018-10-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, plant water extracts are different from a single compound, with complex components, different shades of color, and especially prone to turbidity. Therefore, the above-mentioned conventional detection methods are not suitable for the detection of plant water extracts, and the detection methods need to be improved and optimized.

Method used

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  • A method for rapidly detecting the anti-inflammatory activity of plant water extracts
  • A method for rapidly detecting the anti-inflammatory activity of plant water extracts
  • A method for rapidly detecting the anti-inflammatory activity of plant water extracts

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: This embodiment detects the anti-inflammatory activity of 18 kinds of plant extracts such as cinnamon twig, Cyperus cyperi, Artemisia capriculum, Ligustrum lucidum, Epimedium, and cinnamon, including the following steps:

[0025] (1) Preparation and constant volume of plant extract: 18 kinds of plant raw materials such as cinnamon twig, Cyperus cyperi, Artemisia capillary, Ligustrum lucidum, Epimedium, cinnamon, etc. are ground and pulverized and passed through a 40-mesh sieve. Put it into a Erlenmeyer flask with a mass volume ratio of 1:20, extract it under closed conditions at 80°C and stand still for 6 hours, filter it with neutral filter paper, collect the filtrate, and concentrate at 60°C to a crude drug concentration of 0.5 mg / ul.

[0026] (2) Preparation of substrate: Disperse Tween 20 in 0.2mol / L boric acid buffer at a concentration of 0.5mL / L, then add linoleic acid at a concentration of 0.54mL / L (the purity of linoleic acid used must be >99%), af...

Embodiment 2

[0042] Embodiment 2: the present embodiment takes the rosemary extract as an example to evaluate the precision and repeatability of the detection method of the present invention, as shown in table 3, the present embodiment carries out 4 independent parallel experiments, and calculates the RSD of the inhibition rate (relative standard deviation). The RSD calculation result is 1.17% ( In the formula, S is the standard deviation, is the mean value of inhibition rate.

[0043] Table 3 OD value of rosemary extract inhibiting 5-LOX activity detection

[0044]

Embodiment 3

[0046] In order to accurately quantify the anti-inflammatory activity of plant water extracts, taking Magnolia officinalis water extract as an example, according to the above method, the concentrations of Magnolia officinalis water extracts were detected at 234nm at 25, 125, 250, 500 and 750ug / ul respectively Absorbance OD value at wavelength. 3ug / ul standard quercetin was selected as the reference substance, and the OD values ​​with concentrations of 3, 12, 24, 48 and 60ug / ul were detected respectively. The raw data of the above tests are shown in Table 4.

[0047] Table 4 OD value of Magnolia officinalis water extract and quercetin inhibiting 5-LOX activity detection

[0048]

[0049] The above inhibition rate formula was used to calculate the inhibition rate of different concentrations of Magnolia bark water extracts on 5-LOX enzyme activity. Then, adopt the probit model in the SPSS software regression program (concentration is transformed into lg value), look-up table...

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Abstract

The invention discloses a method for detecting the in vitro anti-inflammatory activity of plant water extracts. The method takes the background of the water extract, which is often neglected in the prior art, as an important detection factor, and overcomes the current cycle of living animal experiments and cell experiments. Long, cumbersome operation, high cost, many interfering factors, and cell experiments cannot directly and independently characterize the anti-inflammatory activity. It has the advantages of simplicity, directness, rapidity, stable results, and low cost. The initial screening work of the object, the whole detection process can be completed within 1 hour. Based on the detection method of the present invention, accurate quantification of anti-inflammatory active ingredients in plant water extracts can be further realized.

Description

technical field [0001] The invention belongs to the technical field of activity detection of water extracts of plants, and specifically relates to the technical field of detection methods for anti-inflammatory activities of water extracts of 18 kinds of plants such as cassia twigs. Background technique [0002] Inflammation is the defense response of the living tissue of the body's vascular system to damage factors, and is tissue damage caused by damage factors. At present, there are mainly two experimental methods of water extracts of plants to study anti-inflammatory activity: one is live animal experiments, which often prepare animal inflammation models through certain inflammatory mediators, and then detect the weight or volume of the inflammatory site and the inflamed tissue. Changes in indicators such as prostaglandin E2, nitric oxide (NO) and malondialdehyde (MDA) to judge the anti-inflammatory activity of plant water extracts, commonly used such as mouse ear swelling...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 陶新徐子伟吴宛澐门小明孙雨晴
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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