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Enzymolysis method for simultaneously extracting fucoidin, alginic acid, mannitol and seaweed dietary fiber

A technology of fucoidan and dietary fiber, which is applied in chemical instruments and methods, food science, food ingredients, etc., can solve the problems of immature technical system, poor extraction effect of functional products, and low application efficiency, and achieve comprehensive improvement. Utilization rate, excellent extraction effect, and reduced degradation effect

Active Publication Date: 2016-10-12
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings of the current biological enzyme preparations in the field of kelp processing, such as low application efficiency, poor extraction effect of functional products, and immature technical system, the present invention solves the above problems through a large number of researches, and provides a method that can simultaneously extract fucoidan, Alginic acid, mannitol and kelp dietary fiber are four functional products of high-efficiency kelp comprehensive utilization special compound enzymatic hydrolysis extraction technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Raw materials

[0050] Made of dried kelp.

[0051] (2) Raw material pretreatment

[0052] Pulverize the raw material of kelp to a particle size of about 40 meshes;

[0053] The crushed raw materials are mixed with water according to the ratio of material to water at 1:20 and fully mixed, and then left to soak for 2 hours.

[0054] (3) One-step compound enzymolysis

[0055] The feed solution that has been left standing and soaked in step (2) is heated to 53°C, the pH of the feed solution is adjusted to 4.9, 0.5% of compound enzyme A (based on the dry weight of kelp) is added, and the temperature is maintained at 53°C for enzymatic hydrolysis for 14 hours. Enzyme A is composed of the following components by mass fraction: 50% cellulase, 30% hemicellulase, 5% pectinase, 1% amylase, and 14% xylanase.

[0056] (4) Two-step compound enzymolysis

[0057] The one-step composite enzymatic hydrolysis solution obtained in step (3) was heated to 58° C., adjusted to pH 7.8...

Embodiment 2

[0072] (1) Raw materials

[0073] Made of fresh kelp.

[0074] (2) Raw material pretreatment

[0075] The kelp raw material is pulverized and the particle diameter is less than 2mm;

[0076] The pulverized raw materials are mixed with water at a ratio of 1:10, and then soaked for 1 hour.

[0077] (3) One-step compound enzymolysis

[0078] The feed solution that has been left standing and soaked in step (2) is heated to 54°C, the pH of the feed solution is adjusted to 5.0, 0.6% of compound enzyme A (based on the dry weight of kelp) is added, and the temperature is maintained at 54°C for enzymatic hydrolysis for 15 hours. Enzyme A is composed of the following components by mass fraction: cellulase 48%, hemicellulase 40%, amylase 0.8%, xylanase 8%, pentosanase 3.2%.

[0079] (4) Two-step compound enzymolysis

[0080] The one-step composite enzymatic hydrolysis solution obtained in step (3) was heated to 57°C, adjusted its pH to 8.2, added composite enzyme B 0.12% (calculated...

Embodiment 3

[0095] (1) Raw materials

[0096] Raw material of waste kelp slag produced by kelp industry

[0097] (2) Raw material pretreatment

[0098] The raw materials are mixed with water at a ratio of 1:25, and then soaked for 2 hours.

[0099] (3) One-step compound enzymolysis

[0100] The feed solution that has been left standing and soaked in step (2) is heated to 53°C, the pH of the feed solution is adjusted to 4.8, 0.75% of compound enzyme A (based on the dry weight of kelp) is added, and the temperature is maintained at 53°C for enzymatic hydrolysis for 12 hours. Enzyme A is composed of the following components by mass fraction: 35% cellulase, 35% hemicellulase, 10% pectinase, 1% amylase, and 19% xylanase.

[0101] (4) Two-step compound enzymolysis

[0102] The one-step composite enzymatic hydrolysis solution obtained in step (3) was heated to 58° C., adjusted to pH 7.8, 0.15% of composite enzyme B (calculated by dry weight of kelp) was added, and the temperature was maintaine...

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Abstract

The invention discloses an enzymolysis method for simultaneously extracting fucoidin, alginic acid, mannitol and seaweed dietary fiber. The method comprises the following steps of firstly performing crushing and water adding soaking on seaweed raw materials; then, adding complex enzymes A for further composite enzymolysis; then, adding complex enzymes B for two-step composite enzymolysis; performing enzyme activity inactivation; performing solid-liquid separation; obtaining solid being seaweed dietary fiber crude products; using obtained liquid for extracting alginic acid, fucoidin and mannitol, wherein the enzymes A contain any four or more than four of cellulose, hemicellulase, pectinase, amylase, xylanase and the like; the complex enzymes B contain one or any two of alkaline protease, neutral protease, papain, trypsin, acid proteinase and pepsin. The method provided by the invention has the most obvious beneficial effect that by using the special composite enzymolysis technology, four kinds of products including fucoidin, alginic acid, mannitol and functional dietary fiber can be simultaneously extracted from seaweeds.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis method for simultaneously extracting fucoidan, alginic acid, mannitol and kelp dietary fiber, and belongs to the technical field of deep processing of marine resources. Background technique [0002] Kelp is a brown algae that can be used for food and medicine. It is very nutritious and rich in a variety of active ingredients that are beneficial to the human body, such as iodine, mannitol, alginic acid, fucoidan, dietary fiber, vitamins, calcium, iron, etc. . my country is a big country of kelp resources and comprehensive utilization. Kelp is a very important marine plant resource in my country and one of the main raw materials of the brown algae industry. A brown algae industrial product system with alginic acid, mannitol and iodine as the main products has been formed. However, due to the fact that my country's current kelp processing industry is mainly based on rough processing, intensive processing a...

Claims

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Application Information

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IPC IPC(8): C08B37/00C08B37/04C07C29/74C07C29/76C07C29/88C07C31/26A23L33/21
CPCA23V2002/00C07C29/74C07C29/76C07C29/88C08B37/0063C08B37/0084A23V2250/202C07C31/26
Inventor 董学前赵林张永刚王伟张艳敏杨晓雪吉武科刘建军
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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