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Method for fermenting ginkgo leaf residues twice to produce agricultural microbial agent

A technology of microbial inoculum and ginkgo leaf residue, applied in the field of agricultural biology, can solve the problems of occupying land space, wasting resources, generating smoke, etc., and achieving the effects of low production cost, easy operation, and easy industrialization

Inactive Publication Date: 2016-10-12
HUBEI NUOKETE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Burial will produce foul smell, and it is not easy to rot for a long time. At the same time, it takes up a lot of land space. Incineration will produce smoke and waste resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) measure 1389kg of ginkgo leaf residue after flavonoids and lactones are extracted by 70% ethanol, measure its water content and be 28%, then contain dry leaf residue 1000kg, supplement 429kg water, make its water content be 45%, simultaneously 5 Dissolve 1 kg of potassium dihydrogen phosphate in water and mix evenly;

[0033] (2) Aspergillus niger 2kg, Trichoderma viride 1kg and Saccharomyces cerevisiae 2kg that effective live bacteria are all 500 million / g are mixed, then connect with (1), mix homogeneously;

[0034] (3) Stack the mixed (2) into a cone with a height of 1.5m and a width of 2.0m;

[0035] (4) Measure the stacking temperature, raise it to above 50°C, turn the stack once every 24 hours, and maintain the cycle for 7 days;

[0036] (5) After the cycle ends, the color of the ginkgo biloba residue turns brown and becomes a loose granular shape.

[0037] (6) measure the decomposed leaf slag water content, control moisture to be 35%;

[0038] (7) metering...

Embodiment 2

[0044] Substantially the same as Example 1, the difference is: the (7) step metering effective viable count is 5 kg of bacillus polymyxa 500 million / g, mixed with 5 kg of bran; the (9) step controls the temperature at about 35°C; (10) The measured effective viable count is 600 million / g.

[0045] Apply the produced agricultural microbial agent to the soil where vegetables have been planted for many years, sprinkle it in when plowing, and use 160kg / mu. Compared with the soil without using the agent, the prevention and control of green pepper fungal diseases can reach about 82%. Yield increased by about 25%.

Embodiment 3

[0047] (1) measure 1389kg of ginkgo leaf residue after 60% ethanol extracts flavonoids and lactones, measure its water content and be 28%, then contain dry leaf residue 1000kg, supplement 611kg water, make its water content be 50%, simultaneously 5 Dissolve one kilogram of potassium dihydrogen phosphate in water, and then mix evenly;

[0048] (2) Aspergillus niger 2kg, Trichoderma viride 1kg and Saccharomyces cerevisiae 2kg that effective live bacteria are all 500 million / g are mixed, then connect with (1), mix homogeneously;

[0049](3) Stack the mixed (2) into a cone with a height of 1.2m and a width of 1.5m;

[0050] (4) Measure the stacking temperature, raise it to above 50°C, turn the stack once every 24 hours, and maintain the cycle for 10 days;

[0051] (5) After the cycle ends, the color of the ginkgo biloba residue turns brown and becomes a loose granular shape.

[0052] (6) measure the decomposed leaf slag water content, control moisture to be 40%;

[0053] (7) me...

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Abstract

The invention relates to a method for fermenting ginkgo leaf residues twice to produce an agricultural microbial agent. The agricultural microbial agent is produced from the ginkgo leaf residues serving as raw materials by two different fermentation processes through different microbial strains, specifically by the following steps: performing primary composting fermentation on the ginkgo leaf residues with a thickness of 1.2 to 15.m by virtue of a mixed strain of aspergillus niger, trichoderma viride and saccharomyces cerevisiae, and inoculating bacillus polymyxa for secondary shallow fermentation with a thickness of 20 to 30cm after thorough decomposition, wherein the living bacterium count of the produced agricultural microbial agent is more than 0.5 billion per gram. A field experiment shows that the agricultural microbial agent can be used for well preventing and controlling pathogenic fungi in soil, reducing soil-borne diseases and promoting growth of crops and has the obvious effect of increasing the yield.

Description

technical field [0001] The invention relates to a method for producing agricultural microbial agent by secondary fermentation of ginkgo leaf residue, which belongs to the field of agricultural biotechnology. Background technique [0002] Ginkgo biloba residue is the solid waste left after the active ingredients such as ginkgo flavonoids and terpene lactones are extracted from ginkgo leaves with alcohol. There are many manufacturers engaged in the production of ginkgo leaf extracts in China, and the leaf residue waste generated every year is about hundreds of thousands tons, the company produces 5000-8000 tons of leaf residue every year. At present, the treatment methods of ginkgo biloba residue include burying, incineration, processing into feed or recycling. Burial will produce foul smell, and it is not easy to rot for a long time. At the same time, it takes up a lot of land space. Burning will produce smoke and waste resources. Since ginkgo biloba residue contains crude ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05F17/00
CPCC05B7/00C05F17/50C05F5/006C05F11/08Y02W30/40
Inventor 石振拓康立新石华杰
Owner HUBEI NUOKETE PHARMA
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