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Kamu Tissue Culture Rapid Breeding Method

A tissue culture, rapid technology, applied in the field of plant breeding, can solve the problems of no kamu tissue, long kamu breeding cycle, etc., and achieve the effect of improving the breeding time, good application prospects, and simple and convenient methods

Active Publication Date: 2018-05-04
SOUTHWEST UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of this, in order to solve the problem that the breeding cycle of camu is long and there is no related technology of camu tissue culture in the prior art, the present invention provides a method for rapid breeding of camu tissue culture

Method used

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Embodiment

[0036] A kind of Kamu tissue culture rapid breeding method, comprises the following steps:

[0037] (1) Annual knotted branches were taken as explants.

[0038] (2) Sterilization of explants: Cut the explants into small sections of 1-1.5 cm, each section needs to be jointed, and shake in 0.1% mercuric chloride solution with 5 drops of Tween-20 per liter for 3 -4min, washed 3 times with sterile water.

[0039] (3) Explant induction: Insert the explant (morphology lower end) through sterilized adventitious bud induction medium (containing 4.0mg / L 6-benzylaminoadenine and 0.2mg / L naphthaleneacetic acid) 1 / 2MS medium).

[0040] (4) Proliferation of adventitious buds: After the explants are cultivated in the adventitious bud induction medium for 30-45 days, 2-3 branches of axillary buds will grow from the nodes, and after each branch reaches 1-3cm, the axillary buds can be cut off , and cultured in the adventitious bud proliferation medium (1 / 2MS medium containing 0.5 mg / L 6-ben...

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Abstract

The invention discloses a rapid breeding method for myrciaria dubia tissue culture. The method includes the following steps that an annual myrciaria dubia branch with nodes is taken and used as an explant; the explant is sterilized and induced; adventitious budpropagation is carried out on axillary buds obtained after inducing; the proliferous axillary buds are cultured to obtain strong seedlings; the strong seedlings are cultured to take root so as to obtain tissue culture seedlings, and the tissue culture seedlings can be transplanted. The technical method of myrciaria dubia tissue culture and rapid breeding is achieved for the first time, and a new path is opened up for the myrciaria dubia tissue culture technology. The rapid breeding method is simple, convenient, easy to implement, short in culture time and capable of effectively shortening myrciaria dubia breeding time and increasing economic benefits. A culture medium and reagents used in the method are easy to prepare and low in cost, and the method has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a rapid breeding method of Kamu tissue culture. Background technique [0002] Camu camu (Myrciaria dubia) is a fruit native to the tropical rainforests of the Peruvian Republic in South America and the Amazon River Basin in Brazil. It is a plant of the genus Myrtaceae. Due to its extremely high vitamin C content (1882-2280mg per 100g fresh weight), it is the well-deserved king of Vc. Every 100g fresh fruit contains 0.4g protein, 5.9g carbohydrate, 0.44g starch, 1.26g soluble sugar, 1.1g dietary fiber, 0.2g fat, 15.7mg calcium, 0.2mg copper, 0.53mg iron, 12.4mg magnesium, manganese 2.1mg, Potassium 83.9mg, Sodium 11.1mg, Zinc 0.2mg. It can be seen that camu camu is rich in nutrition, especially it can be used as a natural Vc source in the food and health care products industry, and has a broad market prospect. In recent years, it has received extensive atten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郭启高梁国鲁党江波李晓林何桥孙海艳向素琼吴頔
Owner SOUTHWEST UNIV
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