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eosin staining solution

A dyeing solution and eosin technology, applied in the field of eosin staining solution, can solve problems such as environmental pollution, physical injury to workers, poisonous chemical reagents, etc., and achieve the effects of increasing water solubility, preventing slice deterioration, and good antibacterial properties

Active Publication Date: 2018-10-09
南京汉瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, not only causing harm to the health of the staff, but also polluting the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An eosin staining solution comprising:

[0018] Water-soluble eosin 12g, water 1200g, sodium chloride 0.4g, citric acid aqueous solution 1.3g of 1mol / L, absolute ethanol 20g, nano-silver 0.01g, glycerol 19g, concentration is 2g of boric acid solution of 5mmol / L, Stearic acid 2g, cetyl alcohol 0.3g, isopropanolamine 1g, Tween 20 2g, allantoin 0.8g, ethylparaben 1g, acrylic resin 6g, tocopheryl acetate 0.8g, lauryl Sodium benzenesulfonate 0.001g and fatty acid glyceride 0.001g.

Embodiment 2

[0020] A method for preparing the eosin staining solution described in Example 1: adding sodium chloride to water at a temperature of 40° C., stirring evenly, heating up to 50° C., adding water-soluble eosin and citric acid aqueous solution, stirring evenly, Add nano-silver while stirring, and in a water bath with a temperature of 30°C, ultrasonicate the water bath for 10 minutes to obtain the first mixed solution;

[0021] Add boric acid solution into absolute ethanol, stir evenly, add sodium dodecylbenzenesulfonate and fatty acid glycerides, in a water bath at a temperature of 30°C, ultrasonicate the water bath for 2 minutes, add glycerin, stearic acid, spermaceti Alcohol, isopropanolamine, Tween 20, allantoin, ethylparaben, acrylic resin and tocopheryl acetate, at a water bath temperature of 40°C, ultrasonication in a water bath for 1 min to obtain a second mixed solution;

[0022] The second mixture was added to the first mixture while stirring, and the temperature of the ...

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PUM

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Abstract

The invention discloses an eosin staining solution. The eosin staining solution is prepared from the following components in parts by mass: 12 parts of water-soluble eosin, 1200 parts of water, 0.4 part of sodium chloride, 1.3 parts of a 1mol / L citric acid water solution, 20 parts of absolute ethyl alcohol, 0.01 part of nano silver, 19 parts of glycerol, 2 parts of a boric acid solution with the concentration of 5mmol / L, 2 parts of stearic acid, 0.3 part of cetyl alcohol, 1 part of isopropanolamine, 2 parts of Tween 20, 0.8 part of allantoin, 1 part of ethylparaben, 6 parts of acrylic resin, 0.8 part of tocopheryl acetate, 0.001 part of sodium dodecylbenzene sulfonate and 0.001 part of fatty glyceride. The eosin staining solution meets the requirement of environment protection of pathological reagents and the staining effect is better than that of a traditional eosin staining solution.

Description

technical field [0001] The invention relates to an eosin staining solution. Background technique [0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, which not only cause harm to the health of the staff, but also pollute the environment. Contents of the invention [0003] It is an object of the present invention to solve at least the above-mentioned problems and to provide at least the advantages which will be described later. [0004] Another object of the present invention is to provide an eosin staining solution, which not only meets the requirements of environmental protection of pathological reagents, but also has better staining effect than traditional eosin staining solution. [0005] In order to achie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 赖保思
Owner 南京汉瑞生物科技有限公司
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