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Citric acid antigen repairing solution

A technology of repairing liquid and citric acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of easy volatilization, antigen destruction, drying of tissue slices, etc., and achieve the effect of not being volatile and good repairing effect

Active Publication Date: 2016-10-05
ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The solvent of the above-mentioned antigen retrieval solution is water. If it is applied to an automatic immunohistochemical staining instrument, on the one hand, the antigen retrieval solution needs to be used in a large amount to achieve a good repair effect, and the tissue section cannot be completely cut with a small amount. On the other hand, it has a low boiling point and is easy to volatilize at a higher temperature, which can easily lead to drying of tissue sections, and once drying occurs, the antigens in tissue sections will become irreversible. Reversed damage beyond repair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A citric acid antigen restoration solution, comprising: 0.378252 g of citric acid monohydrate, 2.411784 g of sodium citrate dihydrate, 500 microliters of Tween-20, 150 mL of triethanolamine and the remainder in the citric acid antigen restoration liquid of every 1000 mL. distilled water.

[0027] The preparation method is as follows: Weigh citric acid monohydrate and sodium citrate dihydrate according to the proportion, dissolve citric acid monohydrate and sodium citrate dihydrate with single distilled water, add triethanolamine in the formula amount, and add Tween- 20. Finally, adjust the volume to the rated capacity with single distilled water.

Embodiment 2

[0029] A citric acid antigen repair solution, each 1000mL of citric acid antigen repair solution includes: citric acid monohydrate 0.378252g, sodium citrate dihydrate 2.411784g, Tween-20 500 microliters, ethylene glycol 325mL and the rest single distilled water.

[0030] The preparation method is as follows: weigh citric acid monohydrate and sodium citrate dihydrate according to the proportion, dissolve the citric acid monohydrate and sodium citrate dihydrate with single distilled water, add the ethylene glycol of the formula amount, and add Tween after completely dissolving -20, and finally adjust the volume to the rated capacity with single distilled water.

Embodiment 3

[0032] A citric acid antigen restoration solution, comprising: 0.35 g of citric acid monohydrate, 2.40 g of sodium citrate dihydrate, 400 microliters of Tween-20, 50 mL of triethanolamine and the remainder in the citric acid antigen restoration solution of 1000 mL. distilled water.

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Abstract

The invention relates to the technical field of immunohistochemistry and discloses a citric acid antigen repairing solution. Each 1000mL of the citric acid antigen repairing solution contains 0.35g-0.40g of citric acid monohydrate, 2.40g-2.42g of sodium citrate dihydrate, 400-600 microliters of Tween 20, 50-500mL of triethanolamine or ethylene glycol or trihydric alcohol and the balance of single-stage distilled water. The citric acid antigen repairing solution has good infiltration and repairing effects and can be used for repairing tissue slices with relatively less use amount; and the repaired tissue slices are easy to stain and the staining effect is good. The repairing solution is not easy to volatilize and can be matched with a full-automatic immunohistochemical instrument for utilization.

Description

technical field [0001] The invention relates to the technical field of immunohistochemistry, in particular to a citric acid antigen retrieval solution. Background technique [0002] Immunohistochemical technique is a detection technique that has developed rapidly in pathological diagnosis in recent years. As an important auxiliary pathological diagnosis method, it has been paid more and more attention in clinical application. With the application and popularization of automatic immunohistochemical staining instruments, the requirements for staining quality are also increasing. Among them, antigen retrieval plays an extremely important role in the entire immunohistochemical staining process, and the quality of the repair conditions directly determines the quality of the final staining results. Due to its own structural characteristics, the full-automatic staining instrument has a small container for holding the repair solution, and cannot fully immerse the tissue sections in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N33/532
CPCG01N1/30G01N33/532
Inventor 李思勇余向东
Owner ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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