Method for quickly detecting nucleic acid of H5 subtype highly pathogenic avian influenza virus
A bird flu virus, highly pathogenic technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of long time consumption
Inactive Publication Date: 2016-10-05
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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Problems solved by technology
At present, various nucleic acid detection methods for H5 subtype highly pathogenic avian influenza virus at home and abroad, including agricultural industry standards or national standards, take a long time, such as agricultural industry standards (RT-PCR detection method for avian influenza virus, NY / T 772–2013) takes at least 2 hours, and the national standard (H5 subtype avian influenza virus fluorescent RT-PCR detection method, GB / T 19438.2–2004) needs at least 1.2 hours. Currently, there is no more efficient detection method than these methods method
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[0008] The following examples illustrate the technical solution of the present invention, but the protection scope of the present invention is not limited to this example.
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Abstract
The invention belongs to the technical field of biology and determines a method for quickly detecting nucleic acid of H5 subtype highly pathogenic avian influenza virus. The method comprises three technical points: determining a primer sequence required for nucleic acid detection; determining detection reaction types; determining a detection reaction system and reaction conditions. The method is useful in scientific research of H5 subtype avian influenza virus and clinical diagnostic detection for animals or humans.
Description
technical field [0001] The invention belongs to the field of biotechnology; specifically, the invention establishes a rapid detection method for H5 subtype highly pathogenic avian influenza virus, which is used for the rapid detection of H5 subtype avian influenza virus, and is used in in vitro diagnosis, veterinary medicine, and food safety. , Biosafety and other fields have greater use value. Background technique [0002] According to different pathogenicity, avian influenza can be divided into highly pathogenic avian influenza, low pathogenic avian influenza and apathogenic avian influenza. Among them, highly pathogenic avian influenza is a disease caused by certain strains of H5 and H7 subtypes. [0003] Viral nucleic acid detection is one of the important methods for virus detection. At present, various nucleic acid detection methods for H5 subtype highly pathogenic avian influenza virus at home and abroad, including agricultural industry standards or national standar...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/701C12Q1/6844C12Q2521/507C12Q2522/101C12Q2537/1376C12Q2521/107C12Q2527/127
Inventor 蒋文明陈继明
Owner CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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