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Method and device for anesthetizing fish

A fish and shellfish, oxygen technology, applied in fish farming, medical science, animal husbandry, etc., can solve the problems of limited practicality and use of anesthesia methods, and achieve the effect of safe anesthesia

Inactive Publication Date: 2016-09-28
MARINE BIOTECH MASSACHUSETTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes a whole day for fish and shellfish to adapt to low temperature (below 5°C) in such a low temperature anesthesia method.
In addition, cooling all ambient water inevitably requires large-scale installations and large power consumption, so the practical use of this anesthesia method is very limited
[0008] On the other hand, when fish and shellfish are anesthetized with carbon dioxide at the water temperature (about 20°C) used to treat fish and shellfish, even if carbonated water for anesthesia containing a sufficient concentration of dissolved oxygen is prepared in advance and then used, it can be used for anesthesia. A device that constantly supplies fresh carbonated water for anesthesia containing a certain concentration of dissolved carbon dioxide gas and dissolved oxygen in a water tank (refer to Patent Document 2), and can only achieve short-term anesthesia for about 20 minutes at the longest

Method used

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  • Method and device for anesthetizing fish
  • Method and device for anesthetizing fish
  • Method and device for anesthetizing fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] "Example 1: Confirmation of the anesthesia limit time for carbon dioxide anesthesia of fish and shellfish at a water temperature of 20°C"

[0040] It is well known that when fish and shellfish are anesthetized with carbon dioxide at the water temperature (about 20°C) at which fish and shellfish are usually treated, the fish and shellfish will die in a very short time even under the condition of saturated dissolved oxygen. The limit time of anesthesia was confirmed experimentally. The types and individual numbers of the fish and shellfish used in the experiment are shown in Table 3. The water temperature in the experimental 700L water tank was adjusted to 20°C, and the dissolved oxygen (DO) in the seawater in the water tank was kept in a saturated state by using a common air pump and an air stone. In the state of saturated dissolved oxygen, carbon dioxide is passed into the water, and the concentration of dissolved carbon dioxide is increased at a rate of 0.5% per minut...

Embodiment 2

[0046] "Example 2: Confirmation of the concentration of carbon dioxide at which anesthesia occurs in fish and shellfish"

[0047] The species and individual numbers of the fish and shellfish used in the experiment are shown in Table 6. Adjust the water temperature in the 700L water tank for the experiment to 20°C, and while using the micro-bubble generating device to continuously supply the micro-bubbles with the particle size distribution shown in Table 5 into the water tank, carbon dioxide is introduced into the water at a rate of increase per minute. A rate of 0.5% increases the concentration of dissolved carbon dioxide to a level that would anesthetize fish and shellfish. The start of anesthesia was evaluated when it was confirmed from the monitoring camera that the swimming action stopped and the body movement stopped except for the breathing movement of the gills. Afterwards, when the carbon dioxide concentration reaches a certain degree higher than the carbon dioxide c...

Embodiment 3

[0055] "Example 3: Verification experiment of long-term anesthesia by carbon dioxide"

[0056] Five three-line sandpipers weighing about 450 g were used in the experiment. Adjust the water temperature in the experimental 700L water tank to 20°C. While continuously supplying microbubbles with the particle size distribution shown in Table 5 into the water tank using the microbubble generator, pass carbon dioxide into the water to increase the concentration of dissolved carbon dioxide. to 5% to anesthetize three-line sea bass. When the concentration of dissolved carbon dioxide reached 5%, it was confirmed by surveillance cameras that all individuals stopped swimming, and their body movements stopped except for the breathing movement of the gills. Thereafter, anesthesia was performed for 20 hours while maintaining a carbon dioxide concentration in the range of 5.0-4.5%. After anesthesia, inject oxygen to drive out the carbon dioxide in the tank, and slowly reduce the concentrati...

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Abstract

To safely and practically simply anesthetize fish over a long period of time in an underwater environment which contains a high concentration of carbon dioxide having an anesthetic effect. By contacting gaseous oxygen-containing microbubbles with the branchial epithelial cell membrane surface of fish, a difference between partial pressures [(partial pressure of gaseous oxygen) - (partial pressure of oxygen dissolved in branchial capillary vessels)], which is larger than a difference between partial pressures [(partial pressure of oxygen dissolved in water) - (partial pressure of oxygen dissolved in branchial capillary vessels)], is created so that the amount of oxygen taken into branchial plate capillary vessels is remarkably increased. Thus, the fish can be anesthetized with carbon dioxide over a long period of time at a water temperature (about 20 DEG C) commonly employed for handling fishes while avoiding respiratory failure that occurs during spontaneous respiratory movements suppressed by the anesthesia.

Description

technical field [0001] The present invention relates to a method and a device for performing long-term anesthesia by using tiny air bubbles containing oxygen to supply oxygen to fish and shellfish in water containing high-concentration carbon dioxide which has an anesthetic effect on fish and shellfish. Background technique [0002] In the past, in places such as fish breeding sites, it was necessary to sedate the fish during the operation in order to prevent damage and strain of the fish body, in order to prevent the fish from being bitten by inoculating vaccines against diseases and cutting teeth to prevent tiger puffer fish from biting each other. Anesthetics are therefore used. Currently, an anesthetic containing isoeugenol (4-propenyl-2-methoxyphenol) as a main ingredient in food additives has been approved and marketed as an animal drug (trade name: FA100), and is used as Anesthetic for fish. However, when it is used in a breeding site or the like, the used anestheti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K63/02A61D7/04
CPCA01K63/02A61D7/04A01K63/042Y02A40/81A01K61/10A01K61/50
Inventor 久木野宪司久木野睦子朝仓富子
Owner MARINE BIOTECH MASSACHUSETTS
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