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Compound bacterium agent for composting straw as well as preparation method and application thereof

A technology of compound bacteria and straw, which is applied in the field of microbial fermentation, can solve the problems of difficult treatment, low energy production, land occupation, etc., and achieve the effects of solving difficulties in transportation and storage of straw, simple preparation method, and good environmental benefits

Active Publication Date: 2016-09-28
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Crop straw is an agricultural product that is difficult to handle. As a cellulose crop, straw has the disadvantages of refractory degradation, long time consumption, and low energy production.
According to the specific national conditions of our country, in the face of the precious biological resources of agricultural straw, the recycling rate in our country is very low, and a large amount of straw is piled up casually or directly burned, which not only occupies land, pollutes the environment, but also causes a serious waste of biomass energy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method for a compound bacterial agent for straw composting:

[0024] PDA medium: potato extract 180g / L, glucose 15g / L, agar 15g / L, deionized water 800mL.

[0025] LB medium: peptone 10g, yeast extract 10g, sodium chloride 5g, deionized water 900mL.

[0026] (1) Rhizopus is inoculated in PDA culture medium, cultivates 50 hours at 28 ℃, shakes with the 0.9% sterilized normal saline of 15ml, obtains that the number of fungal spores is 5 * 10 4 The rhizopus spore suspension of individual / ml;

[0027] (2) Inoculate Bacillus alvei on the LB medium at a temperature of 28°C and a rotation speed of 200rpm, cultivate for 2 days, remove the supernatant after centrifugation to get the precipitate, and resuspend with 15ml of 0.9% sterilized normal saline to obtain the bacterial OD A value of 0.5 for the Bacillus alvei bacteria liquid;

[0028] (3) Trichoderma viride is inoculated in PDA medium, cultivated for 50 hours at 28°C, shaken with 0.9% sterilized physiologic...

Embodiment 2

[0032] A preparation method for a compound bacterial agent for straw composting:

[0033] PDA medium: potato extract 220g / L, glucose 30g / L, agar 25g / L, deionized water 1000mL.

[0034] LB medium: peptone 15g, yeast extract 12g, sodium chloride 10g, deionized water 1000mL.

[0035] (1) Rhizopus is inoculated in PDA culture medium, cultivates 40 hours at 30 ℃, shakes with the 0.9% sterilized normal saline of 30ml, obtains that the number of fungal spores is 7 * 10 4 The rhizopus spore suspension of individual / ml;

[0036] (2) Inoculate Bacillus alvei on LB medium at a temperature of 30°C and a rotational speed of 200rpm, cultivate for 1 day, remove the supernatant after centrifugation to get the precipitate, and resuspend with 30ml of 0.9% sterilized normal saline to obtain bacterial OD Bacillus alvei bacteria liquid with a value of 1;

[0037] (3) Trichoderma viride is inoculated in PDA medium, cultivated for 50 hours at 30°C, shaken with 0.9% sterilized physiological saline...

Embodiment 3

[0041] A preparation method for a compound bacterial agent for straw composting:

[0042] PDA medium: potato extract 200g / L, glucose 22.5g / L, agar 20g / L, deionized water 900mL.

[0043] LB medium: peptone 12.5g, yeast extract 11g, sodium chloride 7.5g, deionized water 950mL.

[0044] (1) Rhizopus is inoculated on PDA medium, cultivates for 45 hours at 29 DEG C, shakes with 0.9% sterilized physiological saline of 25ml, obtains that the number of fungal spores is 6 * 10 4 The rhizopus spore suspension of individual / ml;

[0045] (2) Inoculate Bacillus alvei on LB medium at a temperature of 29°C and a rotation speed of 180rpm, and cultivate for 1.5 days. After centrifugation, remove the supernatant to get the precipitate, and then resuspend with 25ml of 0.9% sterilized normal saline to obtain bacterial OD A value of 0.8 for the Bacillus alvei bacteria liquid;

[0046] (3) Trichoderma viride is inoculated in PDA medium, cultivated for 45 hours at 29°C, shaken with 0.9% sterilize...

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Abstract

The invention discloses a compound bacterium agent for composting straw. The compound bacterium agent is characterized by comprising rhizopus, bacillus pluton, trichoderma viride and phomopsis asparagi B3; the compound bacterium agent contains rhizopus spore suspension with the quantity of fungal spores being no less than 104 / ml, bacillus pluton bacteria liquid with a bacterium OD (Oxygen Demand) value being 0.5 to 1, trichoderma viride spore suspension with the quantity of fungal spores being no less than 104 / ml and phomopsis asparagi B3 spore suspension with the quantity of fungal spores being no less than 104 / ml. The invention further discloses a preparation method of the compound bacterium agent. The same amount of bacterium agents prepared by the four types of bacteria are mixed to prepare the compound bacterium agent; the compound bacterium agent can be used for accelerating the composting speed and composting degree of the straw and improving the regeneration utilization rate of resources; meanwhile, the problems that the straw are difficult to transport and store are solved, atmospheric pollution caused by combustion of the straw is avoided and the environmental benefits are good.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a compound bacterial agent for straw composting and its preparation method and application. Background technique [0002] my country is a large agricultural country, and the total amount of crop resources is showing a continuous growth trend, and the production of crop straw is increasing. Some studies have shown that by 2016, my country's straw production will reach about 900 million tons. Crop straw contains a certain amount of carbon, nitrogen, phosphorus, potassium, calcium, silicon and other elements, and is also rich in organic substances such as cellulose, hemicellulose, lignin and protein. Straw contains a large amount of nutrients that can be used by animals and plants, and is a renewable resource with comprehensive utilization value. [0003] Crop straw is an agricultural product that is difficult to handle. As a cellulose crop, straw has the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/00C12R1/845C12R1/885C12R1/645C12R1/01
CPCC05F3/00C05F17/00C12N1/14C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 沙海涛吴云海明珠
Owner HOHAI UNIV
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