Tissue culture method of dendrobium officinale

Abstract

The invention relates to a tissue culture method of dendrobium officinale; the method comprises the six steps of enabling a round corm to grow on the dendrobium officinale, carrying out proliferation on the round corm, carrying out differentiation and dedifferentiation, culturing strong seedlings, rooting and tempering the seedlings. The method can be used for carrying out tissue culture on the dendrobium officinale by optimizing and improving culture mediums in all the steps, and the culture mediums are respectively an improved 1 / 2 MS basal medium, an MS culture medium filled with potato leaching powder, a 1 / 2 MS culture medium filled with potato leaching powder, an improved culture medium filled with potato leaching powder and NAA, and an improved tissue culture medium filled with a banana extract, NAA and IBA; the culture mediums are concretely dendrobium officinale seed germination culture mediums and improved culture mediums filled with flower treasure No.1 and flower treasure No.2; the dendrobium officinale cultured by the method is high in germination rate and short in germination time, so that the growth speed of tissue cultured seedlings is accelerated, the time consumption is reduced, and the cost is lowered.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a tissue culture method of dendrobium officinale. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It is a precious Chinese medicinal material with a long history of application. It is listed as the first of the nine immortal grasses and is the gold in medicine. "Shen Nong's Materia Medica" records: "Dendrobium dendrobium removes numbness and lowers qi, nourishes the five viscera for deficiency, fatigue and thinness, strengthens yin and improves essence, and thickens the stomach after long-term use." Zhu Danxi, one of the four famous doctors in the Jin and Yuan Dynasties, said that Dendrobium has an outstanding effect of nourishing yin and strengthening yang, and that Dendrobium is unique in traditional Chinese medicine for nourishing yin. Throughout the ages, Dendrobium has been known as ...

AI Technical Summary

Problems solved by technology

In recent years, with the increasing awareness of the medicinal value of Dendrobium candidum, the demand for Dendrobium candidum has also continued to increase. Traditional wild resources can no longer meet people's demand, and even wild resources in some areas are facing depletion.

In order to better protect and utilize the resources of Dendrobium candidum, more and more scientific researchers have begun to respond to the call of the state and began to focus on the research and development of Dendrobium candidum, including artificial domestication and cultivation of Dendrobium candidum, asexual reproduction, etc., tissue culture technology of Dendrobium candidum It is an important means for the reproduction of Dendrobium candidum, and it is also a key way to vigorously promote the planting and propagation of Dendrobium candidum. However, in the general tissue culture process, the seedling growth cycle is long, which consumes a lot of resources and increases the production cost of tissue culture.

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