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Immune cell culture medium

A technology of immune cells and culture medium, which is applied in the field of cell culture, can solve the problems of slow growth rate and achieve the effects of increased expansion speed, good cell function, and maintenance of cell function

Inactive Publication Date: 2016-09-14
GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to achieve rapid expansion of immune cell culture without affecting its function is the key to immune cell culture, but the growth rate of immune cells in the prior art is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An immune cell culture medium, including serum-free basal medium, plasma, cytokines and total glycosides of cistanche. The content of total glycosides of Cistanche cistanche was 5 mg / mL, the volume ratio of plasma and serum-free basal medium was 1:15, cytokines included interleukin-2 (10 ng / mL), interleukin-4 (5 ng / mL), interleukin -5 (10 ng / mL), interleukin-12 (15 ng / mL), interleukin-15 (5 ng / mL), interleukin-23 (5 ng / mL), anti-CD3 antibody (100 ng / mL), anti- CD28 antibody (100 ng / mL) and γ-interferon (10 ng / mL).

Embodiment 2

[0019] An immune cell culture medium, including serum-free basal medium, plasma, cytokines and total glycosides of cistanche. The content of total glycosides of Cistanche cistanche was 10 mg / mL, the volume ratio of plasma and serum-free basal medium was 1:20, cytokines included interleukin-2 (15 ng / mL), interleukin-12 (10 ng / mL), interleukin -15 (15 ng / mL), interleukin-23 (15 ng / mL), anti-CD3 antibody (200 ng / mL), anti-CD28 antibody (200 ng / mL) and γ-interferon (10 ng / mL).

Embodiment 3

[0021] An immune cell culture medium, including serum-free basal medium, plasma, cytokines and total glycosides of cistanche. The content of total glycosides of Cistanche cistanche was 15 mg / mL, the volume ratio of plasma and serum-free basal medium was 1:25, cytokines included interleukin-2 (10 ng / mL), interleukin-5 (15 ng / mL), interleukin -12 (15 ng / mL), interleukin-23 (5 ng / mL), anti-CD3 antibody (100 ng / mL), anti-CD28 antibody (100 ng / mL) and gamma-interferon (5 ng / mL).

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PUM

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Abstract

The invention discloses an immune cell culture medium. The immune cell culture medium comprises a serum-free basal culture medium, plasma, cytokines and cistanchis glycosides. The content of cistanchis glycosides is 5-15 mg / mL, and the volume ratio of plasma to serum-free basal culture medium is (1:15)-(1:25). The cytokines include interleukin, monoclonal antibodies and interferon, the interleukin is one or more of interleukin-1, interleukin-4, interleukin-5, interleukin-12, interleukin-15 and interleukin-23, the monoclonal antibodies are anti-CD3 antibodies or anti-CD28 antibodies or both, and the interferon is gamma-interferon. By the adoption of the immune cell culture medium, different types of immune cells can be cultured, the amplification speed of immune cells is increased greatly, and a good cell function is maintained.

Description

technical field [0001] The invention belongs to the technical field of cell culture, in particular to an immune cell culture medium. Background technique [0002] With the rise of immune cell personalization technology, it is currently believed that the rapid expansion of natural immune cells or genetically modified immune cells in vitro and then reinjected into mammals is conducive to improving the body's immune defense ability, which is manifested in that it can improve the body's immune defense ability. The ability to treat diseases such as tumors, rejuvenate tissues and organs, and prolong the lifespan of the body. How to achieve rapid expansion of immune cell culture without affecting its function is the key to immune cell culture. However, in the prior art, the growth rate of immune cells is slow when cultured. Therefore, it is necessary to develop an immune cell culture medium suitable for the rapid proliferation of immune cells. Contents of the invention [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2501/2302C12N2501/2304C12N2501/2305C12N2501/2312C12N2501/2315C12N2501/2323C12N2501/24C12N2501/51C12N2501/515C12N2501/999
Inventor 陈祥阮雯莉
Owner GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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