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Aerobic denitrifying pseudomonas and application thereof

An aerobic denitrification and pseudomonas technology, applied in bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of single strain and poor effect, and achieve high aerobic denitrification performance Effect

Inactive Publication Date: 2016-09-07
YANGZHOU HAICHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, so far, aerobic denitrification strains are mainly concentrated in the genus Pseudomonas, Bacillus and Aeromonas. The strains are relatively single, and most of the research has focused on degradation genes and degradation mechanisms. The actual effect should not be good.

Method used

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  • Aerobic denitrifying pseudomonas and application thereof
  • Aerobic denitrifying pseudomonas and application thereof
  • Aerobic denitrifying pseudomonas and application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] Pseudomonas, whose classification is named Pseudomonas sp. HC-1, is preserved in the General Microbiology Center of the Chinese Microbial Culture Collection Management Committee, and the depository address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing , Institute of Microbiology, Chinese Academy of Sciences, the postcode is 100101, its deposit number is CGMCC No.11924, and the deposit date is December 24, 2015.

[0026] The Pseudomonas sp. HC-1 was obtained in the following way:

[0027] a. Take the activated sludge at the end of the anoxic section where the SBR reactor has been operating stably for half a month as the separation sludge;

[0028] b. Use the dilution mixed plate method to separate: the isolated strains are placed in a denitrification medium under aerobic conditions for denitrification test, while auxiliary nitrate reduction gas production test and heterochromatic particle staining (methylene blue Dyeing method) inspection. The bacteria that a...

Embodiment 2

[0054] Example 2 Pseudomonas sp. HC-1 denitrification efficiency measurement

[0055] The Pseudomonas sp. HC-1 of the present invention can use a variety of organic matter as a carbon source and can be used for biological denitrification of sewage. The Pseudomonas sp. HC-1 biological denitrification ability was tested, and the Pseudomonas sp. HC-1 strain of the present invention was subjected to the following functional verification:

[0056] 1) Pseudomonas sp. HC-1 growth curve determination.

[0057] Measure the growth curve of Pseudomonas sp. HC-1 according to the standard method of measuring the bacterial growth curve. Take the culture solution every 2h, and use the photoelectric turbidimetric method to measure the OD of the bacterial solution at the wavelength of 660nm 660 (OpticalDensity), and then filtered through 0.22pm microporous membrane to detect the filtrate TN, pH and other indicators. Obtain the growth curve of Pseudomonas sp. HC-1 as figure 1 Shown.

[0058] From figu...

Embodiment 3

[0065] Pseudomonas sp. HC-1 colonies were picked, and the colonies were inoculated into aerobic denitrification medium and cultured at 25° C. for 24 hours, and the obtained fermentation broth was used as the applied bacterial agent. The applied microbial agent is used in the treatment of nitrogen-containing wastewater, and the 24h TN removal rate: 87.2%.

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Abstract

The invention discloses Pseudomonas (sp.) HC-1. The (sp.) HC-1is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 11924, and the preservation date of December 24, 2015. The Pseudomonas (sp.) HC-1 has high aerobic denitrifying property, and is applied to sewage biological nitrogen removal process, the total nitrogen removal rate reaches 88.2 percent, and the characteristics have great significance in the field of water treatment.

Description

Technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering, and relates to an aerobic denitrification strain and its application, and at the same time provides the denitrification efficiency of the strain. Background technique [0002] At present, with the rapid development of the national economy and the continuous deepening of urbanization, the total discharge of urban life and industrial wastewater is also increasing year by year, and the widespread use of pesticides, fertilizers and synthetic detergents has made the concentration of nutrients in the water body continuous Increase, and nitrogen is one of the main reasons for the eutrophication of water bodies. Conventional biochemical treatment processes can effectively reduce BOD and SS in sewage. However, when nutrients such as N and P are present in the sewage, they can only remove 30-40% of the N element in the sewage. A large amount of nitrogen-containing s...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/163C12N1/20C12N1/205C12R2001/38
Inventor 尹安伟范必平查正飞宋丽明
Owner YANGZHOU HAICHENG BIOTECH CO LTD
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