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Conductive parallel fiber membrane capable of promoting rapid repair of peripheral nervous tissues and preparation method of conductive parallel fiber membrane

A peripheral nerve and parallel fiber technology, applied in the field of nerve repair biomaterial preparation, can solve problems such as inability to use in large quantities in clinical use, inability to apply to nerve defects, and difficulty in extracting stem cells, achieving good biocompatibility and functionality, and a simple preparation device , the effect of easy parameter control

Inactive Publication Date: 2016-09-07
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stem cells used in this method cannot be used clinically in large quantities due to the difficulty of extraction and high cost, and the extracellular matrix composite fibers cannot be used in the repair of nerve defects due to their lack of electrical conductivity.

Method used

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  • Conductive parallel fiber membrane capable of promoting rapid repair of peripheral nervous tissues and preparation method of conductive parallel fiber membrane
  • Conductive parallel fiber membrane capable of promoting rapid repair of peripheral nervous tissues and preparation method of conductive parallel fiber membrane
  • Conductive parallel fiber membrane capable of promoting rapid repair of peripheral nervous tissues and preparation method of conductive parallel fiber membrane

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Dissolve 0.18 g of polylactic acid-polycaprolactone in 3 mL of dichloromethane, the viscosity of polylactic acid-polycaprolactone is 2.85 dL / g, stir magnetically at 200 rpm for 4 hours, mix well to obtain a concentration of 60 mg / mL polylactic acid-polycaprolactone electrospinning solution, seal it during stirring and dissolving to prevent solvent volatilization. The obtained electrospinning solution was added to the syringe of the electrospinning device, and the ambient temperature was 18 °C, the spinneret voltage was 10 kV, the spinning distance was 10 cm, the spinning solution injection speed was 1.0 mL / h, and the drum Electrospinning is carried out under the condition of rotating speed of 900 rpm, and the drum with a diameter of 12 cm is wrapped with aluminum foil to receive the fiber filaments. After 2 hours, the electrospun polylactic acid-polycaprolactone with oriented structure can be received on the aluminum foil. silk fiber membrane. Peel off the parallel po...

Embodiment 2

[0033] Dissolve 0.24 g of polylactic acid-polycaprolactone in 3 mL of dichloromethane, the viscosity of polylactic acid-polycaprolactone is 2.85 dL / g, stir magnetically at 300 rpm for 3 hours, mix well, and obtain a concentration of 80 mg / mL polylactic acid-polycaprolactone electrospinning solution should be sealed during stirring and dissolving to prevent solvent volatilization. The electrospinning solution was added to the syringe of the electrospinning device, and the ambient temperature was 25 °C, the spinneret voltage was 11 kV, the spinning distance was 10 cm, the injection speed of the spinning solution was 1.8 mL / h, and the rotation speed of the drum was Under the condition of 600 rpm, electrospinning is carried out, and the drum with a diameter of 12 cm is used to wrap the aluminum foil to receive the fiber filaments. After 1.5 hours, the electrospun polylactic acid-polycaprolactone with a parallel orientation structure is received on the aluminum foil. silk fiber mem...

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Abstract

The invention relates to a conductive parallel fiber membrane which is capable of promoting rapid repair of peripheral nervous tissues and is connected to an extracellular matrix, wherein the thin film (the fiber membrane) can serve as a biofunctional nerve scaffold. The conductive parallel fiber membrane which is connected to the extracellular matrix and is of a core-shell structure is prepared by virtue of a three-step method, namely preparing parallel fibers through electrostatic spinning, covering conductive polypyrrole through electrochemical oxidation, and conducting human skin fibroblast culture and splitting cells by virtue of a triton x-100 / ammonia water mixed solution. According to the invention, the composite fiber membrane, by virtue of the parallel structure, can guide the growth of nerve fibers in a directional mode, by virtue of the extracellular matrix, adhesion of the nerve cells and axon thereof is improved, and by virtue of the conductive polymer (the conductive polypyrrole), the growth of the nerve axon is promoted through electric stimulation; and with a synergistic effect among the three means, the regeneration and repair of nerves are promoted. A preparation device adopted by the invention is simple and easy, simple to operate, wide in material source and low in cost; and the prepared nerve scaffold is good in biocompatibility and functionality, and the nerve scaffold is expected to be applied to clinical field in the future.

Description

technical field [0001] The invention relates to the field of preparation of nerve repair biomaterials, in particular to a conductive parallel composite fiber membrane connected with an extracellular matrix, and the fiber membrane obtained by the preparation method can quickly repair peripheral damaged nerve tissue under the action of electrical stimulation . Background technique [0002] Compared with ordinary material processing methods, electrospun fibers can better simulate the three-dimensional network extracellular matrix structure of the human body, and have great application potential in tissue engineering. Electrospun fibers with an oriented structure can provide good morphology information for peripheral nerve regeneration, that is, guide the regenerated nerve bundles to grow along the fiber direction, which plays an important role in the correct growth of regenerated nerves into the target tissue, and has an important effect on the function of damaged nerve tissue....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/36A61L27/54D04H1/728D04H1/4382D01D5/00
CPCA61L27/18A61L27/3633A61L27/54A61L2300/412A61L2430/32D01D5/003D01D5/0092D04H1/4382D04H1/728C08L67/04C08L79/04
Inventor 黄忠兵杨安能尹光福蒲曦鸣廖晓明
Owner SICHUAN UNIV
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