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Preparation and application of hollow spherical-segment-shaped mesoporous silica/chloroperoxidase nanoreactor

A technology of mesoporous silica and chloroperoxidase, applied in chemical instruments and methods, immobilized on or in inorganic carriers, water pollutants, etc., can solve the problem of high porosity, mesoporous materials and enzyme molecules Problems such as size mismatch, achieve good degradation ability, improved acid and alkali tolerance, and mild reaction conditions

Active Publication Date: 2016-08-31
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the advantages of large specific surface area and high porosity, mesoporous materials are widely used in the immobilization of enzymes, but there are problems such as the mismatch between the size of mesoporous materials and enzyme molecules in the immobilization of enzymes.

Method used

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  • Preparation and application of hollow spherical-segment-shaped mesoporous silica/chloroperoxidase nanoreactor
  • Preparation and application of hollow spherical-segment-shaped mesoporous silica/chloroperoxidase nanoreactor
  • Preparation and application of hollow spherical-segment-shaped mesoporous silica/chloroperoxidase nanoreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of hollow spherical mesoporous silica

[0031] Add 1 g of P123 with a number average molecular weight of 5800, 3.7 mL of concentrated HCl (37% by mass), 30 mL of deionized water into a 100 mL beaker, and then add 2.5 g of 1,3,5-trimethylbenzene and 5.0 g of n-decyl alkane, stirred to completely dissolve P123, then added 2g of tetraethyl orthosilicate, reacted with magnetic stirring at 40°C for 20 hours, then transferred to a hydrothermal reaction kettle, reacted with hydrothermal reaction at 100°C for 30 hours, cooled to room temperature, and filtered with suction , fully filtered and washed with deionized water and absolute ethanol, respectively, and then vacuum-dried at 80°C for 10 hours, the resulting product was placed in a muffle furnace and calcined at 550°C for 4 hours, the template agent P123 was removed, and cooled to room temperature to obtain Hollow spherical mesoporous silica.

[0032] 2. Immobilized Chloroperoxidase

[0033] Add 5mg of holl...

Embodiment 2

[0036]In step 1 of Example 1, 1 g of P123 with a number average molecular weight of 5800, 3.7 mL of concentrated HCl (37% by mass), 30 mL of deionized water were added to a 100 mL beaker, and then 1.5 g of 1,3,5- Trimethylbenzene and 6g of n-decane were stirred to completely dissolve P123, then 2g of tetraethyl orthosilicate was added, and the reaction was carried out under magnetic stirring at 40°C for 20 hours, then transferred to a hydrothermal reaction kettle, and hydrothermally reacted at 100°C for 30 hour, cooled to room temperature, suction filtered, fully filtered and washed with deionized water and absolute ethanol respectively, and then vacuum-dried at 80°C for 10 hours, and the resulting product was calcined in a muffle furnace at 550°C for 4 hours to remove the template Agent P123, cooled to room temperature to obtain hollow spherical mesoporous silica. The other steps were the same as in Example 1 to obtain a hollow spherical mesoporous silica / chloroperoxidase nan...

Embodiment 3

[0038] In step 1 of Example 1, 1.5 g of P123 with a number average molecular weight of 5800, 3.7 mL of concentrated HCl (37% by mass), 30 mL of deionized water were added to a 100 mL beaker, and then 3 g of 1,3,5- Trimethylbenzene and 4.5g of n-decane were stirred to completely dissolve P123, then 2.5g of tetraethyl orthosilicate was added, and the reaction was carried out under magnetic stirring at 40°C for 20 hours, then transferred to a hydrothermal reaction kettle, and hydrothermally heated at 100°C React for 30 hours, cool to room temperature, filter with suction, fully filter and wash with deionized water and absolute ethanol respectively, then vacuum dry at 80°C for 10 hours, and place the product in a muffle furnace for calcination at 550°C for 4 hours. The template agent P123 was removed, and cooled to room temperature to obtain hollow spherical mesoporous silica. The other steps are the same as in Example 1, and the hollow spherical mesoporous silica / chloroperoxidase...

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Abstract

The invention discloses preparation and application of a hollow spherical-segment-shaped mesoporous silica / chloroperoxidase nanoreactor. According to the nanoreactor, tetraethoxysilane serves as a silicon source, P123 serves as a template agent, and a compound of n-decane and 1,3,5-trimethylbenzene serves as an additive; a hydrothermal synthesis method is utilized for preparing hollow spherical-segment-shaped mesoporous silica firstly, then the mesoporous silica serves as a fixed carrier for fixedly carrying chloroperoxidase. The preparation method is simple, reaction conditions are mild, the obtained mesoporous silica is in a hollow spherical segment shape, the specific surface area is large, the mesoporous diameter range is about 14-18 nm, unimolecular fixation of the chloroperoxidase can be realized, and certain turnover space is reserved for the chloroperoxidase. The hollow spherical-segment-shaped mesoporous silica / chloroperoxidase nanoreactor shows good degradation capacity on alizarin red, the degradation rate still can reach 100% after 8 times of use, the high temperature to which the nanoreactor is resistant is increased to about 50 DEG C, and the acid-base resistance is also improved.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, and in particular relates to a method for single-molecule immobilization of chloroperoxidase on a hollow spherical mesoporous silica carrier material, and a nanoreactor prepared by the method in degrading alizarin red Applications. Background technique [0002] Chloroperoxidase (CPO) is a heme peroxidase (42kDa) isolated from the marine fungus Caldariomyces fumago. CPO has a wide range of oxidation activities, including heme peroxidase (one-electron and two-electron peroxidation, organic halogenation), catalase (hydrogen peroxide disproportionation, monohydric alcohol oxidation) and cytochrome P-450 ( Dealkylation, ethylenic bond epoxidation, sulfonation oxidation, etc.) and other enzymes have catalytic characteristics, and are currently considered to be the most widely used enzymes in the peroxidase family. However, free CPO has very poor tolerance to strong acid and alkali, high...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34
CPCC02F3/342C02F2101/308C12N11/14
Inventor 蒋育澄程涵萍胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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