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Microbial agent for tobacco and preparation method and application of microbial agent

A technology for microbial inoculants and cigarettes, applied in the application field of microbial technology

Inactive Publication Date: 2016-08-31
福建美家园生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, it is recognized that the effective way to improve the quality of tobacco is to degrade the macromolecular harmful substances in tobacco and transform small molecular substances into beneficial components through microorganisms. Research on the degradation of the above cell wall components (cellulose, hemicellulose, lignin and pectin) has rarely been reported so far

Method used

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  • Microbial agent for tobacco and preparation method and application of microbial agent
  • Microbial agent for tobacco and preparation method and application of microbial agent
  • Microbial agent for tobacco and preparation method and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Bacteria preparation

[0043] The freeze-dried tubes of Candida utilis and Bacillus subtilis strains were taken respectively for activation, and cultured on the inclined plane of test tubes and shake flasks to prepare microbial bacterial agents.

[0044] Potato culture medium is used for the slant and shake flask culture of Candida utilis, and the slant culture condition is 30° C. for 48 hours. Shake flask culture conditions: 30°C, 180r / min, 48 hours;

[0045] The slant and shake flask culture of Bacillus subtilis adopts the slant culture condition of beef extract peptone medium: 35°C, 24-36 hours. Shake flask culture conditions: 35°C, 180r / min, 24-48 hours.

[0046] Configure bacteria solution

[0047]Candida utilis and Bacillus subtilis were formulated into 2000 g of microbial inoculum at a mass ratio of 1:1, and a total content of 0.5% of inorganic salts was added.

[0048] Tobacco leaf spraying and fermentation

[0049] Spray 1000g of the above-mentioned microb...

Embodiment 2

[0056] Bacteria preparation

[0057] The freeze-dried tubes of Candida utilis and Bacillus subtilis strains were taken respectively for activation, and cultured on the inclined plane of test tubes and shake flasks to prepare microbial bacterial agents.

[0058] Potato culture medium is used for the slant and shake flask culture of Candida utilis, and the slant culture condition is 30° C. for 48 hours. Shake flask culture conditions: 30°C, 180r / min, 48 hours;

[0059] The slant and shake flask culture of Bacillus subtilis adopts the slant culture condition of beef extract peptone medium: 35°C, 24-36 hours. Shake flask culture conditions: 35°C, 180r / min, 24-48 hours.

[0060] Configure bacteria solution

[0061] Candida utilis and Bacillus subtilis were formulated into 2000 g of microbial inoculum at a mass ratio of 1:1, and a total content of 0.5% of inorganic salts was added.

[0062] Bacteria spraying and tobacco leaf fermentation

[0063] Spray 2000g of the above-mentio...

Embodiment 3

[0073] (1) Preparation of bacteria agent

[0074] The freeze-dried tubes of Candida utilis and Bacillus subtilis strains were taken respectively for activation, and cultured on the inclined plane of test tubes and shake flasks to prepare microbial bacterial agents.

[0075] Potato culture medium is used for the slant and shake flask culture of Candida utilis, and the slant culture condition is 30° C. for 48 hours. Shake flask culture conditions: 30°C, 180r / min, 48 hours;

[0076] The slant and shake flask culture of Bacillus subtilis adopts the slant culture condition of beef extract peptone medium: 35°C, 24-36 hours. Shake flask culture conditions: 35°C, 180r / min, 24-48 hours.

[0077] (2) Configure bacterial liquid

[0078] Candida utilis and Bacillus subtilis were formulated into 2000 g of microbial inoculum at a mass ratio of 2:1, and a total content of 0.5% of inorganic salts was added.

[0079] (3) Tobacco leaf spraying and fermentation

[0080] Spray 2000g of the a...

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Abstract

The invention discloses a microbial agent for tobacco and a preparation method and application of the microbial agent, and belongs to the field of application of the microbial technology. The microbial agent formed by candida utilis and bacillus subtilis is evenly sprayed to tobacco leaves and cut tobacco, cellulose, hemicelluloses, pectin, protein, starch and other harmful macromolecular precusor substances in the tobacco can be effectively degraded, therefore, the aim of reducing free radicals, polycyclic aromatic hydrocarbon, nitrogen nitrosamine and other main harmful ingredients in tar of cigarettes is achieved, and tobacco quality is improved.

Description

technical field [0001] The invention relates to the field of microbial technology application, in particular to a method for degrading harmful substances in tobacco by adding microbial agents. Background technique [0002] The technical background related to the present invention will be described below, but these descriptions do not necessarily constitute the prior art of the present invention. [0003] The chemical components and factors affecting the quality and flavor of tobacco are extremely complex, but the harmful components that have not been effectively degraded by natural or artificial fermentation are mainly cell wall components that account for 28% of the dry weight of tobacco itself: cellulose, hemicellulose, lignin , pectin, they seldom undergo degradation changes during the whole storage, alcoholization and processing of tobacco. No matter the tobacco itself, the microorganisms in the fermentation of tobacco or the biologically active enzymes have no ability t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A24B15/18C12R1/125C12R1/72
CPCC12N1/20A24B15/183C12N1/16
Inventor 陈五岭杜茂林
Owner 福建美家园生物科技股份有限公司
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