Composite protein peptide with skin care efficacy, hydrogel thereof and preparation method
A technology of compound protein and compound protease, which is applied in the field of compound protein peptide and its hydrogel and preparation, can solve the problems of inability to improve skin structure, inability to form a protective film, weakening of anti-oxidation and moisturizing effect, and prevent skin dryness Wrinkles and aging, inhibition of melanin production, good appearance and luster
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Embodiment 1
[0053] A kind of composite protein peptide hydrogel of this embodiment is prepared from the following components in terms of mass percentage:
[0054]
[0055]
[0056] The preparation method of above-mentioned silk fibroin peptide comprises the following steps:
[0057] (1) the complete silk fibroin (silkworm cocoon shell) that has been degummed is placed in 50wt% CaCl 2 In aqueous solution (silk fibroin and CaCl 2 The mass ratio of the aqueous solution is 1:18), heated to 80° C., stirred and dissolved, and after the solution was cooled, dialyzed with water for desalination, and concentrated to obtain a silk fibroin solution with a concentration of 10 wt %;
[0058] (2) Adjust the pH value of the silk fibroin solution to 5, add 1000U / g compound protease (35wt% alkaline protease+65wt% papain), adjust the enzymolysis temperature to 30°C, and enzymolysis for 4 hours;
[0059] (3) After enzymatic hydrolysis, heat to 100° C. to inactivate the enzyme, and freeze-dry the mixed...
Embodiment 2
[0065] A kind of composite protein peptide hydrogel of this embodiment is prepared from the following components in terms of mass percentage:
[0066]
[0067]
[0068] The preparation method of above-mentioned silk fibroin peptide comprises the following steps:
[0069] (1) the complete silk fibroin (silkworm cocoon shell) that has been degummed is placed in 50wt% CaCl 2 In aqueous solution (silk fibroin and CaCl 2 The mass ratio of the aqueous solution is 1:10), heated to 80°C and stirred to dissolve. After the solution is cooled, dialyze and desalt with water, and concentrate to obtain a silk fibroin solution with a concentration of 10 wt%.
[0070] (2) Adjust the pH value of the silk fibroin solution to 7, add 1600U / g compound protease (23wt% papain+77wt% trypsin), adjust the enzymolysis temperature to 25°C, and enzymolysis for 6.5 hours;
[0071] (3) After enzymatic hydrolysis, heat to 100° C. to inactivate the enzyme, and freeze-dry the mixed solution to obtain ...
Embodiment 3
[0078] A kind of composite protein peptide hydrogel of this embodiment is prepared from the following components in terms of mass percentage:
[0079] serial number
name
content
1
3%
2
Bovine Casein Peptides
3%
3
0.6%
4
Additives (75% Xanthan Gum + 25% Glycerin)
2%
5
0.5%
6
water
margin
[0080] The preparation method of above-mentioned silk fibroin peptide comprises the following steps:
[0081] (1) the complete silk fibroin (silkworm cocoon shell) that has been degummed is placed in 50wt% CaCl 2 In aqueous solution (silk fibroin and CaCl 2 The mass ratio of the aqueous solution is 1:15), heated to 80°C and stirred to dissolve. After the solution is cooled, dialyze and desalt with water, and concentrate to obtain a silk fibroin solution with a concentration of 10 wt%.
[0082] (2) Adjust the pH value of the silk fib...
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