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Primer, probe and kit for evaluating anti-wrinkle condition of skin

A kit and skin technology, applied in the field of beauty, can solve problems such as difficult to achieve personalized maintenance or treatment

Inactive Publication Date: 2016-08-24
FUJIAN AIWO HEALTH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above patents / patent applications all use foreign substances to regulate the expression of genes in the body, but there are different SNP sites in the genes of each individual. This method is difficult to achieve personalized maintenance or treatment

Method used

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  • Primer, probe and kit for evaluating anti-wrinkle condition of skin
  • Primer, probe and kit for evaluating anti-wrinkle condition of skin
  • Primer, probe and kit for evaluating anti-wrinkle condition of skin

Examples

Experimental program
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Effect test

Embodiment 1

[0079] Example 1: Primer and probe design

[0080] 1. Design and synthesize the following primers and probes. Synthesis unit: Applied Biosystems, USA Applied Biotechnology Co., Ltd.

[0081] ELN (rs2071307) primer and fluorescent probe sequence:

[0082]

[0083] EMILIN1(rs2011616) primer and fluorescent probe sequence:

[0084]

[0085] FLG (rs146466242) primer and fluorescent probe sequence:

[0086]

[0087] FLG (rs200519781) primer and fluorescent probe sequence:

[0088]Forward primer GATCCTTGTCTTCGTCCAGT SEQ ID NO: 13;

[0089] Reverse primer AGCAGAGCCACCAAGAGTCC SEQ ID NO: 14;

[0090] Fluorescent probe VIC-TCCAGACCTTCCCCC T GACCAGT-MGB SEQ ID NO: 15;

[0091] MMP3 (rs3025058) primer and fluorescent probe sequence:

[0092] Forward primer TCATATCAATGTGGCCAAATATTT SEQ ID NO: 16;

[0093] Reverse primer CCTGCCTCAACCTTCAAAAGTGCT SEQ ID NO: 17;

[0094] Fluorescent probe VIC-CAAGACATGG T TTTTTCCCCC-MGB SEQ ID NO: 18;

[0095] MMP1 (rs1799750) primer and ...

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Abstract

The invention discloses a primer, probe and kit for evaluating the anti-wrinkle condition of skin. The sequences of the primer and the probe are SEQ ID NO:1-4, SED ID NO:5-8, SEQ ID NO:9-12, SEQ ID NO:13-15, SEQ ID NO:16-18, SEQ ID NO:19-21 and SEQ ID NO:22-25 wherein the 5' ends and the 3'ends of SEQ ID NO:3-4, SEQ ID NO:7-8, SEQ ID NO:11-12, SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:21 and SEQ ID NO: 24-25 are connected with different fluorescence markers respectively. The invention further discloses a method for assisting a user in selecting a correct beautifying method through gene detection. The internal cause and the external cause of wrinkles of skin of a customer are leant, a user-friendly beautifying healthy solution is provided, and the effects of anti-wrinkle beautifying products are maximized.

Description

technical field [0001] The invention relates to the cosmetic field, in particular to a primer, a probe and a kit for evaluating skin anti-wrinkle conditions. Background technique [0002] SNP (single nucleotide polymorphism) refers to the variation of a single nucleotide on the genome, including transitions, transversions, deletions, and insertions. It is the most common genetic marker, and its carrying frequency in the population is greater than 1%. , and are widely used in the fields of human disease diagnosis, individualized drug use, and basic biological research. With the in-depth study of different SNP sites, through the combined detection of multiple SNP sites and subsequent data analysis, personalized health solutions can be formulated for the genetic information of different groups of people. [0003] The appearance of human skin is affected by internal and external factors. External factors include ultraviolet rays, environmental pollution, improper use of beauty ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 纪志梁王毛阳杨志云刘晓瑜
Owner FUJIAN AIWO HEALTH BIOTECH CO LTD
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