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Selenium enrichment fermentation method for improving indole-3-carbinol and indole-3-acetonitrile in fruit vinegar

A fermentation method and an indole technology, which are applied in the field of beverage processing, can solve the problems of low anti-cancer efficacy components, low anti-cancer efficacy components, and low organic selenium content, so as to reduce the toxicity of selenium, promote the transformation efficiency, and have rich nutrition. Effect

Active Publication Date: 2016-08-24
恩施硒圣植物科技有限公司
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AI Technical Summary

Problems solved by technology

[0005] At present, there are researches on selenium-enriched fruit vinegar, such as the patents with application number 201510821414.5, patent numbers CN200810155868.3 and CN200910043461.6, but without selenium-enriched fermentation, the content of organic selenium in fruits and vegetables is low, resulting in anti-cancer efficacy ingredients in products Low; Patent Nos. CN200410011259.2 and CN200410011258.8 disclose a vinegar fermentation method that uses microorganisms to transform and enrich organic selenium, but does not add anti-cancer substance precursors, and the anti-cancer efficacy components are not high
These methods increase the efficacy of organic selenium, but none of the components that have direct anti-cancer effects

Method used

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  • Selenium enrichment fermentation method for improving indole-3-carbinol and indole-3-acetonitrile in fruit vinegar

Examples

Experimental program
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Embodiment 1

[0021] (1) Take 2kg of apples, wash them with tap water, rinse with distilled water, crush the apples with a juicer, add 5g of pectinase, process at 38°C, stir once every 30 minutes, and adjust the sugar content to 22 with sucrose after 3 hours %.

[0022] (2) Weigh 1kg of cabbage, wash it with tap water, rinse it with distilled water, crush the cabbage with a juicer, add 5g of pectinase and 6g of cellulase, and treat at 42°C for 5 hours, stirring every 30 minutes during the process.

[0023] (3) Fully mix the fruit hydrolyzate in step (1) with the cabbage hydrolyzate in step (2) (sugar content 12%), add 130g of active dry yeast and 1.0mg of sodium selenite, and conduct closed alcoholic fermentation for 36 hours to obtain wine mash.

[0024] (4) Add 150 mL of overnight cultured acetic acid bacteria (OD600=0.5) and 1.0 mg of sodium selenite to the fermented wine mash in step (3) for acetic acid fermentation, stirring once every 6 hours, after 24 hours Start to measure the aci...

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Abstract

The invention discloses a selenium enrichment fermentation method for improving indole-3-carbinol and indole-3-acetonitrile in fruit vinegar, and belongs to the field of beverage processing. The method comprises the preparation steps that 1, fruits are cleaned and crushed, saccharose is added for adjusting the sugar degree, and pectinase is added for hydrolyzing pectin; 2, Chinese cabbages are cleaned and smashed, and pectinase and cellulose are added for hydrolyzing pectin and cellulose; 3, fruit hydrolysate and Chinese cabbage hydrolysate are mixed; 4, sodium selenite and yeast are added for alcoholic fermentation; 5, acetic bacteria are used for acetic fermentation to prepare fruit vinegar; 6, blending is carried out; 7, filtration sterilization is carried out; 8, filling is carried out. The selenium content of each 100 ml of the product produced by the method is 35 micrograms or above, the oxygen radical scavenging efficiency is 4-5 times that of common fruit vinegar, and the fruit vinegar is rich in indole-3-carbinol and indole-3-acetonitrile and is the excellent anticancer healthcare fruit vinegar.

Description

technical field [0001] The invention belongs to the field of beverage processing and relates to a selenium-rich fermentation method for increasing indole-3-carbinol and indole-3-acetonitrile in fruit vinegar. Background technique [0002] Since selenium-enriched food has certain effects in maintaining intracellular redox balance, improving body immunity, anti-cardiovascular disease and anti-cancer, it has become a direction of food nutrition research and development. The intake of selenium is not yet clearly defined. WHO The recommended intake for adult men and women are 40μg / day and 30-40μg / day respectively. [0003] The anti-cancer properties of cruciferous plants are mainly attributed to the breakdown products of glucosinolates contained in cruciferous plants. Cabbage is a cruciferous vegetable. Chinese cabbage in my country has a huge annual output. The terrain in the central and southern regions of my country is relatively high, which makes it difficult to store cabbag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12J1/04
CPCC12J1/04
Inventor 胡勇徐宁李冬生周梦舟祁勇刚高冰汪超石勇徐梅朱于鹏龚元元马俊
Owner 恩施硒圣植物科技有限公司
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