Method for determining pathogenicity of phytophthora nicotianae through pumpkin chips

A technology of Phytophthora tabacum and Phytophthora tabacum, applied in the direction of microorganism-based methods, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of high cost, labor and time, and achieve pathogenicity performance The effect of stability, shortening the experimental period, and the method is simple and easy

Inactive Publication Date: 2016-08-17
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

Because the mycelium block inoculates the leaves quickly, it is more suitable for measuring the pathogenicity of the bacteria, but the pathogenicity of the bacteria cannot be distinguished, and it is not suitable for the simultaneous determination of the pathogenicity and the pathogenicity of the bacteria.
The other two methods both use potted living tobacco seedlings to measure. Due to the long time for cultivating tobacco seedlings and the large space occupied, it is labor-intensive, time-consuming, and expensive, which is not conducive to large-scale experiments. The range is relatively wide, and environmental factors cause some fluctuations in the severity of the disease, and the stability and repeatability of the experiment need to be improved

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  • Method for determining pathogenicity of phytophthora nicotianae through pumpkin chips

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with the examples, but the present invention is not limited in any way, and any transformation or substitution made based on the teaching of the present invention belongs to the protection scope of the present invention.

[0011] The method for measuring the pathogenicity of Phytophthora nicotiana in pumpkin slices of the present invention comprises the steps of preparation of Phytophthora nicotiana mycelium block, preparation of pumpkin slices, inoculation, pathogenicity determination and evaluation, specifically comprising:

[0012] A. Preparation of Phytophthora nicotiana mycelium block: inoculate Phytophthora nicotianae onto OA medium plate, cultivate in dark at 25-28°C for 7-10 days, cut the plate mycelia into mycelial blocks;

[0013] B. Preparation of pumpkin slices: the pumpkin is cleaned and surface sterilized, the exocarp is removed, the endocarp, skin flesh and seeds are removed, the mesocarp...

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Abstract

The invention discloses a method for determining pathogenicity of phytophthora nicotianae through pumpkin chips. The method comprises the following steps of 1, preparation of phytophthora nicotianae hypha blocks; 2, preparation of pumpkin chips; 3, inoculation; 4, pathogenicity determination and evaluation. The method is simple, convenient to use and easy to implement; pathogenic bacteria are stable in pathogenicity expression, repeatability is good, the result is reliable, and the pathogenicity and pathogenic strength of phytophthora nicotianae can be accurately reflected; during actual operation, batch operation can be conducted, the method has the advantages that the occupied space is small, labor cost is reduced, and the experimental period is shortened, and therefore the method is suitable for application and popularization.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for measuring the pathogenicity of Phytophthora tabacum in pumpkin slices. Background technique [0002] Phytophthora nicotianae (Phytophthora nicotianae) is a pathogenic oomycete that causes tobacco black shank. It is seriously harmful to tobacco production and is one of the main pathogenic bacteria affecting tobacco yield and quality. At present, there are mainly three methods for determining the pathogenicity of Phytophthora tabacum: (1) Determination of pathogenicity by sticking mycelium blocks at the base of the stem of the wounded and non-traumatized tobacco seedlings (Wang Ge, Zheng Xiaobo, Lu Jiayun, etc. Yunnan Research on pathogenicity differentiation of Tobacco black shank bacteria in the province, Journal of Nanjing Agricultural University. 1997,20(4): 30-35.); (2) Determination of pathogenicity of tobacco seedlings inoculated with fungus...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/645
CPCC12Q1/04
Inventor 方敦煌黄昌军刘萍花肖炳光陈学军杨大海
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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