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Biological zymocyte for treating pigs dying of diseases and preparation method of biological fermentation zymocyte

A technology for biological fermentation inoculants and dead pigs is applied in the field of biological fermentation inoculants for processing sick and dead pigs and in the field of preparation, and achieves the effects of wide source of raw materials, reasonable ratio and low cost

Inactive Publication Date: 2016-08-17
GUANGXI NANNING SHENGNONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the present invention, aiming at the existing problems in the treatment of sick and dead pigs, a biological fermentation agent and preparation method for treating sick and dead pigs are provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A biological fermentation agent for treating sick and dead pigs, which consists of the following strains: 10kg of Saccharomyces cerevisiae, 20kg of Bacillus subtilis, 5kg of Bacillus thuringiensis, 25kg of actinomycetes, 10kg of Aspergillus niger and 4kg of Aspergillus oryzae.

[0022] Its preparation method comprises the following steps:

[0023] (1) Primary seed culture: Inoculate Saccharomyces cerevisiae, Bacillus subtilis, Bacillus thuringiensis, actinomycetes, Aspergillus niger and Aspergillus oryzae that have been activated by strains into PDA medium respectively, and the culture control temperature is 25°C and pH 6.0, the time control of primary seed culture: Saccharomyces cerevisiae 72h, Bacillus subtilis 36h, Bacillus thuringiensis 48h, actinomycetes 60h, Aspergillus niger 72h and Aspergillus oryzae 48h, then add sterile water respectively in the medium, in Shake at 150r / min for 20min to obtain a primary seed suspension with a weight concentration of 15%;

[0...

Embodiment 2

[0029] A biological fermentation agent for treating sick and dead pigs, which consists of the following strains: 20kg of Saccharomyces cerevisiae, 15kg of Bacillus subtilis, 10kg of Bacillus thuringiensis, 20kg of actinomycetes, 8kg of Aspergillus niger and 8kg of Aspergillus oryzae.

[0030] Its preparation method comprises the following steps:

[0031] (1) Primary seed culture: Inoculate the activated Saccharomyces cerevisiae, Bacillus subtilis, Bacillus thuringiensis, actinomycetes, Aspergillus niger and Aspergillus oryzae into PDA medium respectively, and the culture control temperature is 26°C, pH 5.0, the time control of primary seed culture: the culture time of Saccharomyces cerevisiae is controlled at 60h, Bacillus subtilis 48h, Bacillus thuringiensis 36h, actinomycetes 48h, Aspergillus niger 72h and Aspergillus oryzae 60h, then add no Bacteria water, shaking at a rotating speed of 200r / min for 15min to obtain a first-grade seed suspension with a weight concentration o...

Embodiment 3

[0037] A biological fermentation agent for treating sick and dead pigs, which consists of the following strains: 15kg of Saccharomyces cerevisiae, 25kg of Bacillus subtilis, 8kg of Bacillus thuringiensis, 30kg of actinomycetes, 15kg of Aspergillus niger and 6kg of Aspergillus oryzae.

[0038] Its preparation method comprises the following steps:

[0039] (1) First-level seed culture: inoculate Saccharomyces cerevisiae, Bacillus subtilis, Bacillus thuringiensis, actinomycetes, Aspergillus niger and Aspergillus oryzae that have been activated by strains into PDA medium respectively, and the culture control temperature is 28°C and pH 7.5, the time control of primary seed culture: Saccharomyces cerevisiae 48h, Bacillus subtilis 48h, Bacillus thuringiensis 36h, actinomycetes 72h, Aspergillus niger 96h and Aspergillus oryzae 72h, then add sterile water respectively in the medium, in Oscillate for 10 minutes at a rotating speed of 250r / min to obtain a primary seed suspension with a w...

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PUM

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Abstract

The invention discloses biological zymocyte for treating pigs dying of diseases and a preparation method of the biological zymocyte. The biological zymocyte is prepared from, by weight, 10-20 parts of saccharomyces cerevisiae, 15-25 parts of bacillus subtilis, 5-10 parts of agritol, 20-30 parts of actinomycetes, 8-15 parts of aspergillus niger and 4-8 parts of aspergillus oryzae. Multiple microbial strains are prepared into the biological zymocyte for treating the pigs dying of diseases, the strains are reasonably matched, the synergistic effect is good, the living bacteria count of the biological zymocyte is 2.0*109 cfu / g, the complete decomposition time of muscular tissue of the dead pigs is about 15 days, all organic matter is basically decomposed within about 10 days, and environment-friendly and harmless treatment of the pigs dying of diseases can be achieved.

Description

technical field [0001] The invention relates to the technical field of harmless treatment of sick and dead animals, in particular to a biological fermentation agent for treating sick and dead pigs and a preparation method thereof. Background technique [0002] my country is a big country in the animal husbandry industry, and its breeding scale ranks among the top in the world. The annual pig breeding volume reaches about 1.2 billion. In the process of pig breeding, disease is one of the biggest causes of death. According to data, the number of pigs that die due to disease accounts for about 8-12% of the total number of pigs in my country every year. It can be seen that the number of sick and dead pigs that need to be treated innocuously every year in our country is astonishing. The current main measures for the harmless treatment of sick and dead pigs include deep burial, incineration, chemical production, and biological fermentation. In the actual application process, deep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F1/00C05F17/00C12R1/01C12R1/125C12R1/07C12R1/865C12R1/685C12R1/69
CPCC05F1/005C05F17/00C12N1/14C12N1/18C12N1/20C05F11/08Y02W30/40Y02A40/20
Inventor 苏林戴光文黄东玲邹超贤
Owner GUANGXI NANNING SHENGNONG TECH DEV
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