A strain for promoting the growth of P. with leaves and its application

A technology of Phyllostachys chinensis and plants, which is applied in the direction of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of lack of growth-promoting strains and difficulties in the isolation and cultivation of mycorrhizal fungi, and achieve the promotion of seedling raising efficiency, process, and cost Inexpensive, easy-to-use effect

Active Publication Date: 2019-04-23
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difficulty in the isolation and cultivation of mycorrhizal fungi of the genus Paphiopedilum, there is still a lack of more significant growth-promoting strains.

Method used

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  • A strain for promoting the growth of P. with leaves and its application
  • A strain for promoting the growth of P. with leaves and its application
  • A strain for promoting the growth of P. with leaves and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 : Isolation, identification and cultivation of mycorrhizal fungi

[0022] 1.1 Isolation and purification medium

[0023] The culture medium for isolating mycorrhizal fungi is potato dextrose medium (PDA), 200 g of potatoes, 6 g of agar, 15 g of glucose and replenishing water to 1000 ml, and the pH is natural. According to the conventional biological test method, prepare the separation medium, sterilize under high pressure at 121°C for 20 minutes, wait for the medium to cool to about 45°C, add streptomycin (100ug / ml) and penicillin (100ug / ml), shake well and prepare flat.

[0024] 1.2 Isolation and purification methods of mycorrhizal fungi

[0025] Isolation of mycorrhizal fungi follows the conventional tissue separation method: take the fresh vegetative roots of P. phylloplum, select and observe under a Leica microscope to select root segments with mycelium clusters, rinse them under running water, and rinse them with 75% alcohol for 8 minutes respectively...

Embodiment 2

[0032] Example 2 : Preparation and application of bacterial agent

[0033] 2.1 Preparation and application of liquid bacterial agent

[0034] PDA liquid medium (200 g of potatoes, 15 g of glucose and water to 1000 mL) was sterilized at 121° C. for 20 minutes by high-pressure damp heat, and the medium was cooled to room temperature. After inoculating the strain A6-2, shake the bacteria on a shaking table at 160-220rpm for about 20 days, dilute the bacterial solution 200-300 times, dip or irrigate the roots of the recently transplanted tissue culture seedlings, and irrigate the roots once every 60 days. The requirement for tissue cultured seedlings to emerge from the bottle is that the root length should be at least 2 cm and the plants with more than 3 leaves can be transplanted.

[0035] 2.2 Preparation and application of solid bacterial agent

[0036] The cultivation medium (pine bark about 2m 2 Size: Perlite 4:1) Soaked until fully saturated with water, then sterilized a...

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Abstract

The invention belongs to the technical field of plant growth-promoting bacteria, and in particular relates to a bacterial strain for promoting the growth of P. pachyderm plants and an application thereof. A bacterial strain that promotes the growth of Papudendron pallidum plants, its taxonomic name is Glycobacterium A6‑2 (Tulasnella sp.), and it was preserved in the Guangdong Microbial Culture Collection Center on May 12, 2016, with a preservation number of GDMCC No. 60034. The application of the growth-promoting bacterial strain of P. pedioides plants of the present invention in promoting the growth of P. The method provided by the invention is used to promote the growth of the Papudendron phyllophyllum plant, and the operation is simple and the cost is low. The invention has important economic value for the artificial breeding of P.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and in particular relates to a bacterial strain for promoting the growth of P. pachyderm plants and an application thereof. Background technique [0002] Paphiopedilum is one of the most popular orchid plants with peculiar flower shape and gorgeous color. People often call Paphiopedilum "slipper orchid" and "cinderella". In the past ten years, due to rampant smuggling and habitat destruction, the number of wild Paphiopedilum has decreased sharply, and its distribution area has gradually shrunk. Many species have reached the verge of extinction. [0003] In order to alleviate the pressure on the collection of wild plants, protect endangered species, and meet people's needs for Paupelopera plants, scholars at home and abroad have conducted a lot of research on the artificial propagation of Paphiopedilum plants. At present, the aseptic sowing and tissue culture techniques of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00
CPCA01N63/30C12N1/145C12R2001/645
Inventor 王晓国周主贵黄昌艳闫海霞卢家仕李秀玲何荆洲李冬萍邓杰玲陈廷速张自斌卜朝阳
Owner FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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