Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for establishment of non-alcoholic fatty liver disease model by utilizing ApoE-/-mice

A non-alcoholic, model-building technology, applied in the field of non-alcoholic fatty liver disease animal model establishment, can solve the problems of long molding cycle and poor stability, and achieve the effect of good stability, high repeatability and reliable method.

Inactive Publication Date: 2016-08-17
DALIAN UNIV
View PDF5 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these animal models can reflect the pathogenesis of non-alcoholic fatty liver to a certain extent, most of them have the characteristics of poor stability and long modeling period

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishment of non-alcoholic fatty liver disease model by utilizing ApoE-/-mice
  • Method for establishment of non-alcoholic fatty liver disease model by utilizing ApoE-/-mice
  • Method for establishment of non-alcoholic fatty liver disease model by utilizing ApoE-/-mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of the animal model of the present invention

[0018] 1. Experimental animals: SPF male AopE- / - mice, 6-8 weeks old, body weight (22±2) g, purchased from Changzhou Cavens Laboratory Animal Co., Ltd., Jiangsu Province [SCXK (苏)2011-003 ]. SPF male C57BL / 6 mice, 6 to 8 weeks old, body weight (22±2) g, purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. [SCXK (Beijing) 2011-0011].

[0019] 2. The experimental animals are kept in the barrier environment of this department, the temperature is 22~25℃, the humidity is 50%, and the day and night alternate for 12 hours.

[0020] 3. After 1 week of adaptive feeding, the experimental animals were randomly divided into four groups, each with 8 animals. The detailed grouping situation is shown in Table 1. The model was built for 10 weeks with a fixed dose of feed in the morning and evening, guaranteed 40g / cage, 4 mice per cage, free water during the period, the water source was SPF sterilized...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the establishment of an animal model of non-alcoholic fatty liver disease, in particular to the establishment of a non-alcoholic fatty liver animal model induced by high fat accompanied by severe blood fat abnormality. SPF-grade male AopE- / - mice and C57BL / 6 mice were selected, randomly divided into model group and control group according to the species of mice, a total of 4 groups, 8 mice in each group, and all mice were adaptively fed with ordinary maintenance feed for 1 week. , the model group was given high-fat feed, and the control group was given control feed. After 10 weeks, the blood was drawn and sacrificed, and the four items of routine blood lipids and liver function were tested; Liver tissue was made into paraffin and frozen sections, and H.E. and oil red O staining were performed respectively to determine the effect and quality of the NAFLD model. The present invention uses a simple high-fat and high-calorie diet to make models, and creates a severe NAFLD model within a very short period of 10 weeks. The model's blood lipids, liver fat and other indicators have excellent stability and high repeatability, which is a high-quality NAFLD model. Establishment provides a reliable method.

Description

Technical field [0001] The present invention relates to the establishment of a non-alcoholic fatty liver disease (NAFLD) animal model, in particular to the establishment of a type of high-fat-induced non-alcoholic fatty liver animal model with severe dyslipidemia. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a clinical pathological syndrome characterized by excessive deposition of fat in liver cells in addition to alcohol and other clear liver damage factors. NAFLD is composed of a series of continuously developing diseases. Only simple fatty liver (SFL) and non-alcoholic steatohepatitis (NASH) occur in the early stage. With the development of the disease, fibrosis of the liver tissue will eventually be triggered and lead to the formation of liver cirrhosis. At the same time, obesity and dyslipidemia caused by NAFLD are also the culprits for other metabolic disorders, such as atherosclerosis (AS), hypertension and type 2 diabetes. With the p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K50/50A23K20/158
CPCA01K67/02
Inventor 刘庆平路遥林家彬王仁军
Owner DALIAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com