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A kind of Lactobacillus plantarum resistant to Salmonella enteritidis infection and application thereof

A technology of Salmonella enteritidis and Lactobacillus plantarum, applied in the field of microbiology, can solve severe sepsis, death and other problems, achieve good inhibitory ability, reduce infection, and improve growth

Active Publication Date: 2019-10-15
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the infected target is an infant, the elderly, or a group with weak resistance, and has symptoms such as severe diarrhea and fever, antibiotics must be used for treatment, otherwise it may cause more serious sepsis, etc., and even cause death.

Method used

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  • A kind of Lactobacillus plantarum resistant to Salmonella enteritidis infection and application thereof
  • A kind of Lactobacillus plantarum resistant to Salmonella enteritidis infection and application thereof
  • A kind of Lactobacillus plantarum resistant to Salmonella enteritidis infection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Physiological curve experiment of embodiment 1 Lactobacillus plantarum CGMCC No.11446

[0029] MRS medium is a medium well known to those skilled in the art, and it contains peptone fish meal, yeast extract, glucose, sodium acetate, diammonium citrate, Tween 80, magnesium sulfate, manganese sulfate, pH 6.2-6.4.

[0030] Inoculate the Lactobacillus plantarum CGMCC No.11446 that has entered the stable growth phase in the MRS liquid medium at an inoculation amount of 2% based on the mass of the MRS liquid medium, and culture it at 37°C. Take samples every 2 hours and measure the OD600 value, pH value and supernatant inhibited Salmonella enteritidis activity, obtained as attached figure 1 Physiological curve of Lactobacillus plantarum CGMCC No.11446 shown.

[0031] attached by figure 1 It can be seen that Lactobacillus plantarum CGMCC No.11446, cultured at 37°C, enters the mid-log phase at 6 hours, enters the stable phase at 16 hours, at this time the OD600 value is the la...

Embodiment 2

[0032] Example 2 Analysis of the inhibitory ability of Lactobacillus plantarum CGMCC No.11446 to Salmonella enteritidis strains

[0033] LB culture medium was poured into a plate (15 mL per plate, and the plate was a standard plate of 9 mm), and the Salmonella enterica CCFM9161, Salmonella enterica CCFM9162, Salmonella enterica CCFM9163 and Salmonella enterica CCFM9164 (Salmonella enterica) CCFM9164 ( The above-mentioned experimental strains are all from the bacterial strain bank of the Food Biotechnology Center of Jiangnan University) and spread on the plate (the concentration is 1×10 8 CFU / plate), put it into 4 Oxford cups immediately, and add 100 μL of Lactobacillus fermentation broth to the Oxford cups, diffuse at 4°C for 1 hour, put it in a 37°C incubator for 24-28 hours, and measure the inhibition zone size. Using MRS as a negative control, the experiment was repeated three times, and the test results are listed in Table 1.

[0034] Table 1 Analysis of inhibition of gr...

Embodiment 3

[0038] Example 3 Component analysis of Lactobacillus plantarum CGMCC No.11446 inhibiting Salmonella enteritidis

[0039] In the same manner as in Example 2, the agar diffusion test was used to detect the inhibition of the growth of Salmonella enteritidis by the live and dead bacteria resuspended in the fermentation broth, supernatant and PBS of Lactobacillus plantarum CGMCC No. 11446 of the present invention. The test results show that (see figure 2 ), the fermentation liquid and the cell-free supernatant of Lactobacillus plantarum have significant ability to inhibit the growth of Salmonella enteritidis, and the fermentation liquid shows a stronger antibacterial effect than the cell-free supernatant. This result shows that, in addition to the antibacterial substances in the supernatant, the live bacteria also have a certain antibacterial effect, which may be related to competitive growth.

[0040] The effect of pH on the antibacterial effect of the supernatant was studied. ...

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Abstract

The invention provides a plant Lactobacillus (Lactobacillus plantarum) resistant to Salmonella enteritidis infection and application thereof, belonging to the technical field of microbes. The bacterium was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms on September 24, 2015, and the preservation number is CGMCC No.11446. The Lactobacillus plantarum CGMCC No. 11446 of the present invention has better properties of acid resistance and bile salt resistance, and has significant inhibitory effect on the growth of Salmonella enteritidis. It has a strong inhibitory effect on the adhesion of Salmonella Enteritidis to intestinal epithelial cells HT-29, and has the effect of preventing or alleviating Salmonella Enteritidis infection. The Lactobacillus plantarum CGMCC No. 11446 can be used to prepare pharmaceutical compositions for preventing or alleviating Salmonella enteritidis, food and fermented food containing active lactic acid bacteria, and has very broad application prospects.

Description

technical field [0001] The invention relates to plantarum lactobacillus capable of intervening Salmonella enteritidis infection and application thereof, belonging to the technical field of microbes. Background technique [0002] Salmonella enterica (Salmonella enterica) is a typical Gram-negative pathogen, which mainly causes human enteritis, food poisoning and gastroenteritis of livestock and poultry, seriously threatening the health of humans and animals. Salmonella Enteritidis is a pathogenic bacterium without a specific host. This bacterium can directly cause poultry disease and cause serious economic losses, and can also indirectly contaminate poultry as a carrier of Salmonella Enteritidis, eventually endangering humans. Mellroy SG et al. reported that 40%-80% of food poisoning incidents in developed countries such as the United States and Japan were caused by Salmonella avian, mainly Salmonella Enteritidis. In March 1989, the World Health Organization (WHO) held an em...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A23C9/123A23C9/127A23C13/16A23C19/00A23C11/10A23C20/02A23L11/00A23L19/00C12R1/25A23L11/45A23L11/50A23L11/60
CPCA23C9/123A23C9/127A23C11/106A23C13/16A23C19/00A23C20/025C12N1/20A61K2035/115C12R2001/25C12N1/205A23V2400/169
Inventor 刘晓军马杰张建军张凌宇陈卫张灏翟齐啸
Owner BEIJING SCITOP BIO TECH CO LTD
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