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Preparation of graphene-based composite material and application thereof in chemiluminiscence detecting DNA (Deoxyribonucleic Acid) content

A chemiluminescence detection and composite material technology, which is applied in the field of photochemical sensors, can solve the problems of poor selectivity, fragile, and time-consuming measurement, and achieve the effects of easy control of conditions, simple preparation process, and strong adsorption capacity.

Inactive Publication Date: 2016-07-20
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] With the development and cross-penetration of different disciplines such as biology, chemistry, physics, electronics, and computers, a variety of DNA detection methods have been established, including the traditional PCR polymerase chain reaction, which has relatively high positioning, selectivity, and multiple detection Poor; colorimetry, relying on the color of the end point to judge the DNA content, there is a large error; mass spectrometry, because the ionization efficiency of oligonucleotides is low and easy to break; fluorescence detection method, the quenching efficiency is low, the sensitivity is not good High; electrochemical method, time-consuming and poor reproducibility: it is particularly important to establish a simple, effective, fast and convenient, high sensitivity, and good selectivity analysis method; DNA detection technology with a large development space
[0005] The flow injection chemiluminescence method in the photochemical method has the advantages of convenient operation, high sensitivity, simple instrument, fast analysis speed, and no pollution. It is widely used in the field of analysis. Assay selectivity is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Fe 3 o 4 SiO 2 Preparation: In a 150mL round bottom flask, add 36mL of ethanol, 4mL of ultrapure water, and the purified Fe 3 o 4 Nanoparticles 0.3000g; ultrasonic oscillation 15~20min, under mechanical stirring, add ammonia water 5mL and tetraethyl orthosilicate 2mL; mechanical stirring reaction at 10°C constant temperature for 12h; product collected by external magnetic field; placed in 50°C for vacuum drying box for drying; after drying, grind;

[0017] (2) Fe 3 o 4 SiO 2 Synthesis of / GO composite material: take 0.2g graphene oxide, dissolve in 100mL ultrapure water, add 3mg1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 28mg hydroxysuccinate Imide; magnetic stirring for 30min, ultrasonication for 30min to form a uniform dispersion; add 200mg of silanized modified product Fe 3 o 4 SiO 2 -NH 2 , ultrasonic for 30min, stirred at 80°C for 2h, and magnetically separated (washed with ultrapure water and ethanol respectively); placed in a 50...

Embodiment 2

[0023] (1) Fe 3 o 4 SiO 2 Preparation: In a 150mL round bottom flask, add 36mL of ethanol, 4mL of ultrapure water, and the purified Fe 3 o 4 Nanoparticles 0.3000g; ultrasonic oscillation 15~20min, under mechanical stirring, add ammonia water 5mL and tetraethyl orthosilicate 2mL; mechanical stirring reaction at 10°C constant temperature for 12h; product collected by external magnetic field; placed in 50°C for vacuum drying box for drying; after drying, grind;

[0024] (2) Fe 3 o 4 SiO 2 Synthesis of / GO composite material: take 0.2g graphene oxide, dissolve in 100mL ultrapure water, add 3mg1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 28mg hydroxysuccinate Imide; magnetic stirring for 30min, ultrasonication for 30min to form a uniform dispersion; add 200mg of silanized modified product Fe 3 o 4 SiO 2 -NH 2 , ultrasonic for 30min, stirred at 80°C for 2h, and magnetically separated (washed with ultrapure water and ethanol respectively); placed in a 50...

Embodiment 3

[0030] (1) Fe 3 o 4 SiO 2 Preparation: In a 150mL round bottom flask, add 36mL of ethanol, 4mL of ultrapure water, and the purified Fe 3 o 4 Nanoparticles 0.3000g; ultrasonic oscillation 15~20min, under mechanical stirring, add ammonia water 5mL and tetraethyl orthosilicate 2mL; mechanical stirring reaction at 10°C constant temperature for 12h; product collected by external magnetic field; placed in 50°C for vacuum drying box for drying; after drying, grind;

[0031] (2) Fe 3 o 4 SiO 2 Synthesis of / GO composite material: take 0.2g graphene oxide, dissolve in 100mL ultrapure water, add 3mg1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 28mg hydroxysuccinate Imide; magnetic stirring for 30min, ultrasonication for 30min to form a uniform dispersion; add 200mg of silanized modified product Fe 3 o 4 SiO 2 -NH 2 , ultrasonic for 30min, stirred at 80°C for 2h, and magnetically separated (washed with ultrapure water and ethanol respectively); placed in a 50...

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Abstract

The invention discloses preparation of a graphene-based composite material and application thereof in chemiluminiscence detecting the DNA (Deoxyribonucleic Acid) content. The main technical characteristic is characterized in that according to a method, magnetism is modified on the surface of graphene oxide, and a magnetic graphite base composite material with a conjugate adsorption capacity on a single-stranded DNA molecule is synthetized, the preparation process is simple, the conditions are easy to control, and the production cost is low. The invention provides a new method for detecting DNA. The synthetized Fe3O4@SiO2 / GO composite material can realize quick adsorption on the single-stranded DNA, is strong in adsorption capacity, and high in efficiency; the time for the single-stranded DNA adsorption to achieve the balance is only 10 to 15min, and the maximal adsorption capacity is the saturated adsorption amount achieving the magnitude order 10<-7> to 10 <-6>mol / g. The method for detecting DNA is low in detection line, can achieve 10<-9>mol / L, and has the advantages of high sensitivity, high selectivity, good reproducibility, no pollution on the environment and the like.

Description

technical field [0001] The invention relates to the preparation of a graphene-based composite material and its application technology in chemiluminescence detection of DNA content, which belongs to the technical field of photochemical sensors, and in particular to the preparation of a magnetic graphene oxide composite adsorption material and its application in chemiluminescence The technology of DNA content detection in the system. Background technique [0002] Graphene is a nanomaterial with a two-dimensional crystal structure. Its unique structure, outstanding electrical and thermal conductivity and mechanical properties are widely used in analytical chemistry. However, there is a strong π-π interaction between graphene sheets, which makes Its dispersibility in the solution is seriously affected, and it is prone to coagulation, so it is necessary to modify and modify graphene; graphene oxide (GO) is obtained by modifying graphene, and GO has amphiphilicity. It has the cha...

Claims

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Application Information

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IPC IPC(8): B01J20/20B01J20/28B01J20/30G01N21/76
CPCB01J20/06B01J20/103B01J20/20B01J20/28009B01J2220/42G01N21/76
Inventor 孙元玲罗川南段辉敏王晓娇王延辉李剑波丁超凡
Owner UNIV OF JINAN
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