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Herba violae extract and application thereof

A technology of Radix vulgaris and extract, which is applied in the directions of plant raw materials, medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, etc. Advanced problems, to achieve the effect of low preparation cost

Active Publication Date: 2016-07-20
LUNAN HOPE PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a viola extract to overcome the shortcomings of the existing viola extracts, such as a single effective ingredient, a low content of active ingredients, a high proportion of ineffective impurities, and a cumbersome preparation and purification process for the extract.

Method used

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  • Herba violae extract and application thereof
  • Herba violae extract and application thereof
  • Herba violae extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Antiviral Test of Viola Didin Extract

[0025] 1. Experimental materials

[0026] (1) Test substance

[0027]Viola extract (extract prepared for the best embodiment 3 of the present invention, the content of total coumarin and teretin is 48% and 21% respectively) is brownish black, fragrant, bitter in taste, texture simply. During the test, crush and sieve, take 50 mg, dissolve in 2 mL of phosphate saline, filter and sterilize, and set aside.

[0028] Positive drug: antiviral oral liquid (provided by Jiangxi Qingfeng Pharmaceutical Co., Ltd.), batch number Z20044129, production date is July 21, 2014. Sterilize by filtration and set aside.

[0029] (2) Virus strains

[0030] The herpes simplex virus HSV-1F strain was quoted from the Department of Microbiology of Shandong Medical University in 1996.

[0031] (3) Cell lines

[0032] Human laryngeal carcinoma epithelial cells Hep2.

[0033] 2. Test methods and results

[0034] (1) Cell recovery and passag...

Embodiment 2

[0067] Embodiment 2 viola extract

[0068] (1) Preparation of Viola extract

[0069] Take 5kg of viola herb, extract it twice with 8 times 70% ethanol, each time for 1 hour, filter, concentrate the filtrate under reduced pressure, add water to adjust to 0.5 times the weight of the medicinal material, and add it to the pretreatment at a flow rate of 0.5BV / h On a good HPD100 macroporous adsorption resin column, the amount of resin is 1 / 3 of the amount of medicinal materials. First, use 1BV of purified water to elute at a flow rate of 1BV / h, discard the water eluent, and then use 1BV of 50% ethanol at a rate of 1BV / h. The flow rate of h is eluted, the ethanol eluate is collected, and finally eluted with 1BV70% ethanol at a flow rate of 1BV / h, the ethanol eluate is collected, the ethanol eluate is combined, concentrated under reduced pressure to a relative density of 1.15-1.30, vacuum After drying, 98 g of viola extract was obtained.

[0070] (2) Determination of the content of ...

Embodiment 3

[0081] Embodiment 3 viola extract

[0082] Take 5kg of viola herb, extract twice with 12 times of 70% ethanol, each time for 1 hour, filter, concentrate the filtrate under reduced pressure, add water to adjust to 0.5 times the weight of the medicine, and add it to the pretreatment at a flow rate of 0.5BV / h On a good HPD750 type macroporous adsorption resin column, the amount of resin is 1 / 3 of the amount of medicinal materials. First use 2BV of purified water to elute at a flow rate of 2BV / h, discard the water eluent, and then use 2BV of 50% ethanol at a rate of 3BV / h. The flow rate of h is eluted, the ethanol eluate is collected, and finally eluted with 2BV70% ethanol at a flow rate of 3BV / h, the ethanol eluate is collected, the ethanol eluate is combined, concentrated under reduced pressure to a relative density of 1.15-1.30, vacuum After drying, 98 g of viola extract was obtained.

[0083] According to the method for determining the content of total coumarin and teretin in...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine, and relates to a herba violae extract. The extract is prepared by the following methods: ethanol extraction, decompression concentration, purification with macroporous adsorption resin, concentration, drying and the like. Compared with the prior art, the herba violae extract disclosed by the invention greatly improves the contents of total coumarin and esculetin, wherein the total coumarin content is improved to be above 40% from 1.7% in medicinal materials and the esculetin content is improved to be above 15% from 0.5%. Upon pharmacological tests, T1 of the herba violae extract on herpes simplex viruses is 8, showing that the extract is relatively strong in effect on inhibiting the herpes simplex viruses.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a Viola didin extract and its application. Background technique [0002] Violet is the dry whole plant of Violayedoensis Makino. Harvest in spring and autumn, remove impurities, wash, chop, and use fresh or dried. Zihuadiding is an important medicine for treating furunculosis. "Compendium of Materia Medica" records: "Zihuadiding is mainly used for all carbuncles and gangrene, swollen scrofula, unknown swollen poison, and malignant sores"; A common medicine for furunculosis". Viola dichotoma is bitter, pungent, cold in nature, returns to the heart and liver meridian, has the effects of clearing away heat and detoxification, cooling blood and reducing swelling. [0003] The main active ingredients of Viola didin are coumarins, and the whole plant contains auretin, chicoryin, early opening violaside, kaempferol-3-O-rhamnopyranose, apigenin, luteolin, palmitate...

Claims

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Application Information

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IPC IPC(8): A61K36/86A61P31/12A61P31/22
Inventor 张贵民孙宗喜卢连华冯倩乔建卫
Owner LUNAN HOPE PHARM CO LTD
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