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Tissue engineering double-layered tubular support and preparation method thereof

A tissue engineering and tubular technology, applied in the field of tissue engineering tubular stent and its preparation, can solve the problems that the mechanical properties are difficult to satisfy the tubular tissue, the stent has a single component, etc., and achieves the effects of low preparation cost, simple operation, and improved overall mechanical properties.

Inactive Publication Date: 2013-11-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the scaffold prepared by this method has a single component, and its mechanical properties are difficult to meet the requirements of tubular tissue.

Method used

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  • Tissue engineering double-layered tubular support and preparation method thereof
  • Tissue engineering double-layered tubular support and preparation method thereof
  • Tissue engineering double-layered tubular support and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The inner layer structure is a PLLA / PLCL dense nanofiber structure, and the outer layer structure is a double-layer tubular scaffold of the PLLA / PCL self-forming pore system.

[0028] (1) Dissolve PLLA and PLCL in tetrahydrofuran at 55°C with a mass ratio of 40:60, and stir to prepare a 10% (w / v) homogeneous solution;

[0029] (2) Cast the PLLA / PLCL mixed solution into a polytetrafluoroethylene mold, and quickly place it at -80°C to separate the phases overnight;

[0030] (3) Take out the mold from the low temperature, and remove the mold, soak the gel polymer in the deionized ice-water mixture to exchange the solvent for 2 days, and change the deionized ice water 3 times a day;

[0031] (4) Take out the tubular scaffold from deionized water and freeze-dry to obtain the PLLA / PLCL nanofiber tubular scaffold with dense structure;

[0032] (5) Put the dense nanofiber tubular support prepared in (4) on the stem of the polytetrafluoroethylene mold to assemble a new casting mo...

Embodiment 2

[0038] The inner layer structure is a PLLA / PLCL dense nanofiber structure, and the outer layer structure is a double-layer tubular scaffold with a PLLA porous nanofiber structure caused by sugar spheres.

[0039] (1) Dissolve PLLA and PLCL in tetrahydrofuran at 55°C with a mass ratio of 70:30, and stir to prepare a 10% (w / v) homogeneous solution;

[0040] (2) Cast the PLLA / PLCL mixed solution into a polytetrafluoroethylene mold, and quickly place it at -20°C to separate the phases overnight;

[0041] (3) Take out the mold from the low temperature, and remove the mold, soak the gel polymer in deionized ice water to exchange solvent for 2 days, and change the deionized ice water 3 times a day;

[0042] (4) Take out the tubular scaffold from deionized water and freeze-dry to obtain the PLLA / PLCL nanofiber tubular scaffold with dense structure;

[0043] (5) Put the dense nanofiber tubular support prepared in (4) on the stem of the polytetrafluoroethylene mold to assemble a new ca...

Embodiment 3

[0051] The inner layer structure is a PLLA / PLCL dense nanofiber structure, and the outer layer structure is a double-layer tubular scaffold with a PLLA porous nanofiber structure prepared by a gas foaming method.

[0052] (1) Dissolve PLLA and PLCL in tetrahydrofuran at 55°C with a mass ratio of 50:50, and stir to prepare a 10% (w / v) homogeneous solution;

[0053] (2) Cast the PLLA / PLCL mixed solution into a polytetrafluoroethylene mold, and quickly place it at -80°C to separate the phases overnight;

[0054] (3) Take out the mold from the low temperature, and remove the mold, soak the gel polymer in deionized ice water to exchange solvent for 2 days, and change the deionized ice water 3 times a day;

[0055] (4) Take out the tubular scaffold from deionized water and freeze-dry to obtain the PLLA / PLCL nanofiber tubular scaffold with dense structure;

[0056] (5) Put the dense nanofiber tubular support prepared in (4) on the stem of the polytetrafluoroethylene mold to assemble...

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Abstract

The invention relates to a tissue engineering double-layered tubular support and a preparation method thereof. The support is composed of two polymer layers with different pore structures, the inner layer is a compact nanofiber structure, and the outer layer is of a porous nanofiber structure. The preparation method comprises the following steps of: dissolving a polymer material in a solvent, pouring in a mould, performing phase separation overnight, demoulding to obtain a polymer gel, performing solvent replacement, and freeze drying to obtain a nanofiber tubular support with a compact structure; sleeving the nanofiber tubular support on a mould stem to obtain a casting mould; and dissolving a polymer material in a solvent, casing the polymer solution in the casing mould by combing an electroporation technology, performing phase separation overnight, demoulding, performing solvent replacement, removing the solvent and / or porogen, and freeze drying to obtain the tissue engineering double-layered tubular support. The preparation method disclosed by the invention is simple in operation, free of complicated equipment, suitable for large-scale production and low in preparation cost; the whole mechanical property of the support is improved by introducing other polymers in the phase separation system.

Description

technical field [0001] The invention belongs to the field of tissue engineering tubular support and its preparation, in particular to a tissue engineering double-layer tubular support and a preparation method thereof. Background technique [0002] Higher animals are rich in tubular tissues, such as trachea, blood vessels, lymphatic vessels, and intestines. These tubular tissues have an obvious feature, and their walls are composed of different types of cell layers. Transplantation and reconstruction are generally performed when these tissues are defective or severely damaged. Among the lesions of these tubular tissues, cardiovascular disease is one of the most serious diseases threatening human beings. The clinically used autologous and allogeneic grafts have many defects, such as insufficient donors, easy to cause immune rejection, etc., which limit their application. The development of tissue engineering has provided a new way to construct grafts in vitro that are simila...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/56
Inventor 何创龙王伟忠仇可新冯炜
Owner DONGHUA UNIV
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