Edible fungus protein peptide-selenium chelate preparation method

A technology of edible mushrooms and chelates, which is applied in food science, protein food processing, protein food ingredients, etc., can solve the problems of small lethal dose, low bioavailability, toxic and side effects, etc., and achieve high protein extraction rate and easy to be extracted Effect of absorption, low price

Inactive Publication Date: 2016-07-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Step 1: Superfinely crush the straw mushrooms, mix the crushed straw mushrooms with distilled water at a ratio of w / v1:20, adjust the pH to 9.0 with 2% NaOH, ultrasonically extract for 10 minutes, and extract in a water bath for 60 minutes at a temperature of 60°C. After extraction, filter with gauze, the filtrate is the alkali-extracted straw mushroom protein solution, adjust the pH of the alkali-extracted straw mushroom protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge at 4000r / min for 5min to obtain the precipitated protein, wash with water until neutral, and centrifuge again. The precipitation is freeze-dried to obtain the protein of straw mushroom, and the extraction rate reaches 71.25%.

[0021] Step 2: Dissolve the straw mushroom protein in water so that the protein concentration reaches 3.0%, adjust the pH of the straw mushroom protein solution to 9.0 with NaOH solution, keep the temperature at 50°C, add 3.0% protease, hydrolyze...

Embodiment 2

[0031] Step 1: Ultrafinely crush Pleurotus eryngii, mix the crushed Pleurotus eryngii with distilled water at a ratio of w / v1:60, use 3% NaOH to adjust the pH to 11.0, ultrasonic extraction for 50 minutes, water bath extraction for 120 minutes, temperature 100 ℃, filter with gauze after extraction, the filtrate is the alkali-extracted Pleurotus eryngii protein solution, adjust the pH of the alkaline-extracted Pleurotus eryngii protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge to obtain the precipitated protein, wash with water until neutral, and centrifuge again. Pleurotus eryngii protein was obtained by precipitation freeze-drying, and its extraction rate reached 85.75%.

[0032] Step 2: Dissolve Pleurotus eryngii protein in water so that the protein concentration reaches 3.0%, adjust the pH to 11.0, keep the temperature at 60°C, add 3.0% protease, hydrolyze for 60 minutes, and inactivate the enzyme to obtain the Pleurotus eryngii protein pol...

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PUM

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Abstract

The present invention provides an edible fungus protein peptide-selenium chelate preparation method. The preparation method comprises the following steps: an alkali-extraction and acid-precipitation method or an ammonium sulfate precipitation method is used to extract proteins in edible fungi; alkaline proteases, neutral proteases or compound proteases are used to conduct restriction enzyme digestion for the edible fungi, the enzymes are inactivated, and an edible fungus protein enzymatic hydrolysate is prepared; and selenium in inorganic sodium selenite and edible fungus protein peptides are chelated to obtain the edible fungus peptide-selenium chelate. The alkali-extraction and acid-precipitation method or the ammonium sulfate precipitation method can significantly increase the extraction rate of the edible fungus proteins. By controlling the enzyme digestion time, the degradation degree is effectively controlled. The protein peptide-selenium chelate preparation technology is simple. The prepared fungus protein peptide-selenium chelate has a unique chelation structure and a transport mechanism, is easily absorbed, safe, non-toxic and low in prices, at the same time can supplement amino acids and selenium, and becomes a first choice of a selenium supplement. The preparation method provides a new way for the application of the edible fungi.

Description

technical field [0001] The invention relates to a preparation method of edible mushroom protein peptide-selenium chelate, which belongs to the field of food biotechnology. Background technique [0002] Selenium (Se) is an important mineral element necessary for the human body and has important physiological functions. A large number of existing studies have shown that many diseases are closely related to the nutritional status of Se. The human body mainly obtains selenium through diet, and the method of artificial selenium supplementation is oral selenium-containing preparations. The general method of supplementing selenium is to make oral preparations with inorganic selenium compounds (sodium selenite and sodium selenate). However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited. Studies have shown that organic selenium compounds a...

Claims

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Application Information

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IPC IPC(8): A23L33/00A23J1/00A23J3/34
CPCA23J1/008A23J3/347A23V2002/00
Inventor 赵立娜刘斌陈濠陈紫红肖正吕旭聪刘晓艳林占熺
Owner FUJIAN AGRI & FORESTRY UNIV
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