Culture method capable of reducing chlorella adherence

Abstract

The invention discloses a culture method capable of reducing chlorella adherence.The culture method includes preparing a BG-11 culture solution, and adding a 10-300 ppm emulsifying agent into the BG-11 culture solution to obtain a culture solution capable of reducing chlorella adherence.The culture method capable of reducing chlorella adherence means that the culture solution capable of reducing chlorella adherence is used for culturing chlorella.When cultured chlorella cells reach a stable phase, dry cell weight as high as 875-956 mg can be obtained from every L of culture solution and is increased by 40-66% than a BG-11 chlorella culture solution without the emulsifying agent.The culture solution capable of reducing chlorella adherence has no adverse effect on growth of the chlorella, and accordingly the cultured chlorella is safe and free of toxicity and is particularly suitable as raw materials of health products, feed, food additives, fine chemical products and medical preparations.

Description

technical field [0001] The invention provides a culture method for reducing chlorella adherence by using an emulsifier. Specifically, in the process of culturing Chlorella, adding an appropriate amount of emulsifier to the Chlorella culture solution can effectively reduce the wall-attachment phenomenon of Chlorella, and has no adverse effect on the growth of Chlorella, and the cultured Chlorella The algae are safe and non-toxic. Background technique [0002] Chlorella is a single-cell green algae of the genus Chlorella in the phylum Chlorella. It is widely distributed, has strong environmental adaptability, and a short growth cycle. It is a new type of low-carbon environmental protection resource. Chlorella is rich in protein, polysaccharides, lipids, leaf greens, vitamins and some biologically active substances. It is widely used in health food, feed, food additives, fine chemicals and pharmaceutical preparation raw materials, and has important development potential. [0...

AI Technical Summary

Problems solved by technology

Once the chlorella cells adhere to the wall, it will produce a vicious circle and form adverse effects, causing the growth of chlorella to slow down or even die, the culture effect will continue to decline, and even lead to the collapse of the entire culture system

Someone suggested that the anti-adhesive property of organic silicon can be used to apply it on the surface of the inner wall of the chlorella culture device to reduce the surface adhesion, improve the smoothness and hydrophobicity of the surface, and reduce the adhesion of chlorella, such as patent CN102040909A A method for preparing an organic silicon antifouling material with a low elastic modulus. Although the problem of chlorella's adherence to the wall can be solved, the silicone antifouling material coated on the inner wall surface of the chlorella culture device will block the sunlight. Not conducive to the growth of chlorella

Therefore, in the past production process of Chlorella, there are two ways to deal with it after the adherence occurs: 1. After the Chlorella is harvested, the cleaning of the cultivation device is carried out, such as the cleaning of the inner wall of the photobioreactor tube of the patent CN102000679A pipeline method, although the problem of chlorella sticking to the wall has been solved like this, such a mode has increased manpower and material resources, and has just increased production costs; 2, in the culture solution of chlorella, add defoamer, utilize defoamer The low surface tension and low surface energy characteristics of organic silicon can effectively reduce or prevent the adherence of chlorella, such as the method of reducing or preventing the adherence of microalgae and the cultivation method of microalgae in the patent CN103184155A, although this can also Solve the problem of chlorella sticking to the wall, but it does not explain the metabolism of chlorella in the culture solution containing organic silicon, which will cause more or less organic silicon to remain in the chlorella. If the extracted chlorella is used In terms of consumption such as health care products, food, feed, etc., it will affect the health of humans or animals

[0004] Therefore, how to reduce adherence and ensure the quality of chlorella on the premise of saving cultivation costs is very important in the cultivation process of chlorella. However, so far, there is no way to ensure the quality of chlorella At the same time, it is really effective to prevent the method of wall-attachment during the cultivation of Chlorella

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