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Method of utilizing biosurfactant and microbial agent to treat oil-containing sediment

A microbial agent and biological surface technology, applied in the field of petroleum biotechnology and environmental biology, can solve the problems of environmental pollution, high technical cost, complex process equipment, etc.

Inactive Publication Date: 2016-07-13
BEIJING HUANASI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method overcomes the shortcomings of commonly used physical and chemical methods, such as high technical cost, polluting the environment, and complex process equipment, and broadens a new path for the application field of petroleum hydrocarbon degradation treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Sampling: collect oily mud and sand, air-dry in a cool and ventilated place, sieve to remove impurities, store for later use, and its oil content is 9.13%;

[0036] 2. In the scrubber, according to the solid-to-liquid ratio, add cleaning liquid at 20:1, the cleaning temperature is 37°C, the total mass concentration of biosurfactant is 5%, rhamnolipid: lipopeptide is 4:1, and the stirring speed is at Wash at 200r / min for 45min, let it stand, and separate after stratification;

[0037] 3. Remove the oil slick, pour off the supernatant, dry the cleaned oily mud sand at 37°C, and measure the residual oil content to be 4.58%;

[0038] 4. Add the cleaning solution in the scrubber according to the solid-liquid ratio of 10:1, the cleaning temperature is 37°C, the total mass concentration of the microbial agent is 4%, and the ratio of Pseudomonas aeruginosa: Bacillus subtilis: Bacillus firmus is 4: 1:1, the stirring speed was degraded for 90d under the condition of 180r / min,...

Embodiment 2

[0041] 1. Sampling: Collect oily mud and sand, air-dry in a cool and ventilated place, sieve to remove impurities, save for later use, and its oil content is 6.13%;

[0042] 2. In the scrubber, according to the solid-to-liquid ratio, it is 15: 1 to add cleaning solution, the cleaning temperature is 37 ℃, the total mass concentration of biosurfactant is 4%, rhamnolipid: lipopeptide is 2: 3, and the stirring speed is at Wash at 200r / min for 45min, let it stand, and separate after stratification;

[0043] 3. Remove the oil slick, pour off the supernatant, dry the cleaned oily mud sand at 37°C, and measure the residual oil content to be 3.26%;

[0044] 4. Add cleaning solution in the scrubber according to the solid-to-liquid ratio of 20:1, the cleaning temperature is 37°C, the total mass concentration of microbial agents is 4%, and the ratio of Pseudomonas aeruginosa: Bacillus subtilis: Bacillus firmus is 1: 1:1, the stirring speed was degraded for 45 days under the condition of ...

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Abstract

The invention belongs to the technical field of petroleum biological technology and environment biological technology, and particularly relates to a method of utilizing a biosurfactant and a microbial agent to treat oil-containing sediment.The method includes following steps: inoculating biosurfactant producing bacteria and microbial agent seed liquid which are cultured well through expansion according to an inoculating amount with a volume ratio of 2-8% into a fermentation tank culture medium after being sterilized, wherein the fermentation culture medium contains necessary carbon source, nitrogen source, inorganic salt and trace elements; adjusting pH value of the fermentation culture medium; ventilating, stirring and fermenting to obtain the biosurfactant or the microbial agent.By adopting the method, residual crude oil of oil-containing sediment can be well emulsified, and the method has effect of lowering oil content of the oil-containing sediment.

Description

technical field [0001] The invention belongs to the fields of petroleum biotechnology and environmental biotechnology, in particular, it relates to a method for treating oily mud sand by using biosurfactant and microbial agent. The method of the invention can well emulsify the residual crude oil in the oily mud sand, and at the same time have a good effect of reducing the oil content of the oily mud sand. Background technique [0002] Petroleum is one of the essential energy sources in today's social life and production, and it is also recognized as one of the pollutants that have the most serious impact on the environment. During the exploration, development, processing and transportation of petroleum, a large amount of oily mud sand will be produced. Oily sands usually contain alkanes, aromatic hydrocarbons, non-hydrocarbon compounds, and asphaltenes, which can pollute the ecological environment through soil and groundwater and threaten human health. Therefore, the safe d...

Claims

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Application Information

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IPC IPC(8): C02F11/02C12P19/44C12P21/02C12N1/20C12R1/385C12R1/125C12R1/07
CPCC02F11/02C12N1/20C12P19/44C12P21/02
Inventor 董丁卢彦珍
Owner BEIJING HUANASI TECH
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