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Novel serum metabolite composition and application thereof as liver cancer diagnosis marker

A technology of metabolites and serum, applied in the field of analytical chemistry and clinical medicine, can solve the problems of lack of early warning value, low diagnostic sensitivity and specificity, and limited inspection effect

Active Publication Date: 2016-07-06
HANGZHOU HEALTH BANK MEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the diagnosis of liver cancer mainly includes imaging examination and serum alpha-fetoprotein (AFP) detection, but the former has limited role, and is often found and diagnosed only in the middle and late stages, lacking early warning value; the latter is sensitive and specific in diagnosis The sensitivity is low, and the detection rate is generally only about 50%-75%.

Method used

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  • Novel serum metabolite composition and application thereof as liver cancer diagnosis marker
  • Novel serum metabolite composition and application thereof as liver cancer diagnosis marker
  • Novel serum metabolite composition and application thereof as liver cancer diagnosis marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] 1. Serum sample collection Before the collection, the included volunteers signed the informed consent.

[0087] Inclusion criteria for normal control, liver cirrhosis and primary liver cancer groups: refer to "Huang Jiasi Surgery (7th Edition)" People's Health Publishing House, Beijing, 2008

[0088] Inclusion criteria for the healthy control group: normal physical examination population (between 40 and 70 years old). Physical examination of healthy people.

[0089] Serum samples were used under the same conditions: serum samples from 40 normal persons, 79 cases of liver cirrhosis and 80 cases of liver cancer; within 24 hours after admission, 4ml of fasting anticoagulant blood and 4ml of procoagulant blood were collected, and the serum was separated within half an hour. Store in -80°C refrigerator for future inspection.

[0090] 2. Analysis method

[0091] 2.1 Serum sample pretreatment

[0092]Thaw blood samples at 4°C, take 80 μL of serum samples, add 320 μL of ace...

Embodiment 2

[0106] In order to verify the stability and reliability of the combined markers for the diagnosis of early liver cancer, small liver cancer samples with small tumor diameters were added for verification. The analytical method, instrument and reagent used in this experiment are all completely consistent with those in Example 1.

[0107] 1. Serum sample collection Before the collection, the included volunteers signed the informed consent.

[0108] Inclusion criteria for normal control, liver cirrhosis and primary liver cancer groups: refer to "Huang Jiasi Surgery (7th Edition)" People's Health Publishing House, Beijing, 2008

[0109] Inclusion criteria for the healthy control group: normal physical examination population (between 40 and 70 years old). Physical examination of healthy people.

[0110] Serum samples were used under the same conditions: 40 cases of normal people, 65 cases of liver cirrhosis, 75 cases of small liver cancer patients and 29 cases of large liver cance...

Embodiment 3

[0122] In order to verify the stability and reliability of the combined markers for the diagnosis of early liver cancer, liver cancer patients from different regions than those in Examples 1 and 2 were added to carry out the verification experiment II. The analytical method, instrument and reagent used in this experiment are all completely consistent with those in Example 1.

[0123] 1. Serum sample collection Before the collection, the included volunteers signed the informed consent.

[0124] Inclusion criteria for normal control, liver cirrhosis and primary liver cancer groups: refer to "Huang Jiasi Surgery (7th Edition)" People's Health Publishing House, Beijing, 2008

[0125] Inclusion criteria for the healthy control group: normal physical examination population (between 40 and 70 years old). Physical examination of healthy people.

[0126] Serum samples were used under the same conditions: serum samples from 31 normal persons, 48 ​​cases of liver cirrhosis, and 92 case...

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Abstract

The invention discloses a novel serum metabolite composition and an application thereof as a marker for preparing an early-stage liver cancer diagnosis kit. The serum metabolite composition includes: glycocholic acid, tryptophan, histidine, uridine, choline, benzamide, lysophosphatidyl choline 16:1 and phenylalanyl tryptophan. The serum metabolite composition can be used for early-stage diagnosis of liver cancer, is low in detection cost, is high in repeatability and sensitivity, and has excellent complementarity with a conventional clinical diagnosis marker serum alpha fetoprotein (AFP).

Description

technical field [0001] The invention relates to the application of a new combined marker in the preparation of a diagnostic kit for early liver cancer. It belongs to the field of analytical chemistry and clinical medicine. Background technique [0002] Hepatocellular carcinoma (HCC) refers to malignant tumors that occur in the liver. According to statistics, there are about 600,000 new patients with liver cancer every year. Its incidence rate ranks fifth among malignant tumors and its mortality rate ranks third. The etiology of liver cancer is complex, most of which are caused by hepatitis virus infection. In my country, liver cancer mostly develops on the basis of hepatitis B cirrhosis. Liver cancer is hard to be found due to its inconspicuous early symptoms, and there is no effective treatment for middle and advanced liver cancer, its 5-year survival rate is 7%, and it is only 3.9% at stage IV, so early detection and early treatment are extremely important for liver cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
Inventor 许国旺罗萍尹沛源孔宏伟李艳丽
Owner HANGZHOU HEALTH BANK MEDICAL LAB CO LTD
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