A kind of separation and detection method of pyrimethamine

A technology of pyrimethamine and a detection method, applied in the fields of organic chemistry and analytical chemistry, can solve the problems of weak hydrogen bonding force, limited adsorption capacity of target substances, weak selectivity of template molecules, etc., and achieves increasing binding sites and improving affinity. and selective, reinforcing effects

Active Publication Date: 2018-01-02
SOUTH CHINA NORMAL UNIVERSITY
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Problems solved by technology

[0006] However, due to the weak hydrogen bond force, only 1-2 hydrogen bonds are used to interact in general molecular imprinting techniques, and the adsorption capacity for target substances is limited.
Therefore, there are fewer binding sites between the template molecule and the functional monomer, and the selectivity to the template molecule is weak, so it cannot achieve effective selective adsorption on the template molecule.

Method used

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  • A kind of separation and detection method of pyrimethamine

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Embodiment Construction

[0027] A method for separation and detection of pyrimethamine disclosed by the invention comprises the following steps:

[0028] (1) Sample treatment: add acetonitrile to the sample, shake and mix well, centrifuge, take the supernatant and dry it with nitrogen, and redissolve the residue with a solvent as the test solution.

[0029] (2) Add the magnetic molecularly imprinted polymer into the liquid to be tested, shake for 20-120 min, and magnetically separate the magnetically imprinted polymer.

[0030] (3) Eluting the pyrimethamine in the magnetic molecularly imprinted polymer with a certain volume of elution solvent, and collecting the eluate.

[0031] (4) The concentration of pyrimethamine in the eluate was determined by HPLC / UV.

[0032] In this embodiment, a urine sample is taken as an example. In a 5 mL centrifuge tube, add 1 mL of urine followed by 1 mL of acetonitrile. After shaking for 1-2 minutes, the mixture was centrifuged at 12,000 r / min for 10-30 minutes, the ...

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Abstract

The invention discloses a method for separation and detection of pyrimethamine, which comprises the following steps: (1) adding acetonitrile to the sample, shaking and mixing, centrifuging, taking the supernatant and drying it with nitrogen, and redissolving the residue with a solvent as The solution to be tested; (2) adding the magnetic molecularly imprinted polymer to the test solution, shaking for 20-120 minutes, magnetically separating the magnetically imprinted polymer; (3) eluting the magnetically imprinted polymer with a certain volume of elution solvent pyrimethamine in the eluate, and collect the eluate; (4) measure the concentration of pyrimethamine in the eluate with HPLC / UV; The functional monomer is prepared. The magnetic molecularly imprinted polymer used in the present invention uses multiple hydrogen bond interactions between template molecules and functional monomers to form an intermolecular interaction matrix, strengthen the interaction between template molecules and functional monomers, and improve the affinity for pyrimethamine and selective.

Description

technical field [0001] The invention relates to the fields of organic chemistry and analytical chemistry, in particular to a separation and detection method for pyrimethamine. Background technique [0002] Pyrimethamine (PYR) is currently a widely used antimalarial drug, mainly used for the prevention of malaria. It can inhibit the activity of dihydrofolate reductase in parasites, thereby interfering with the normal metabolism of folic acid in Plasmodium. Protozoan erythrocytes are effective in the early stage and are often used as etiological preventive drugs. It can also inhibit the development of malaria parasites in mosquitoes, so it can block transmission. It is clinically used for the prevention of malaria and anti-relapse treatment of telogen. Malaria is estimated to kill 500 million clinical cases every year, and it is one of the diseases that kill the most people except AIDS and tuberculosis infectious diseases, which has attracted the attention of the Internation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 梁勇
Owner SOUTH CHINA NORMAL UNIVERSITY
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