Separation and detection method of pyrimethamine

A pyrimethamine and detection method technology, applied in the field of organic chemistry and analytical chemistry, can solve the problems of weak hydrogen bond force, limited adsorption capacity of target substances, weak selectivity of template molecules, etc., to increase binding sites and strengthen the effect , Improve the effect of affinity and selectivity

Active Publication Date: 2016-07-06
SOUTH CHINA NORMAL UNIVERSITY
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Problems solved by technology

[0006] However, due to the weak hydrogen bond force, only 1-2 hydrogen bonds are used to interact in general molecular imprinting techniques, and the adsorption capacity for target substances is limited.
Therefore, th

Method used

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  • Separation and detection method of pyrimethamine

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Embodiment Construction

[0027] A method for separation and detection of pyrimethamine disclosed by the invention comprises the following steps:

[0028] (1) Sample treatment: add acetonitrile to the sample, shake and mix well, centrifuge, take the supernatant and dry it with nitrogen, and redissolve the residue with a solvent as the test solution.

[0029] (2) Add the magnetic molecularly imprinted polymer into the liquid to be tested, shake for 20-120 min, and magnetically separate the magnetically imprinted polymer.

[0030] (3) Eluting the pyrimethamine in the magnetic molecularly imprinted polymer with a certain volume of elution solvent, and collecting the eluate.

[0031] (4) The concentration of pyrimethamine in the eluate was determined by HPLC / UV.

[0032] In this embodiment, a urine sample is taken as an example. In a 5 mL centrifuge tube, add 1 mL of urine followed by 1 mL of acetonitrile. After shaking for 1-2 minutes, the mixture was centrifuged at 12,000 r / min for 10-30 minutes, the ...

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Abstract

The invention discloses a separation and detection method of pyrimethamine. The separation and detection method comprises the following steps: (1) adding acetonitrile into a sample; after shaking and uniformly mixing, centrifuging; taking supernatant and blowing with nitrogen gas; dissolving residues with a solvent to obtain a solution to be detected; (2) adding a magnetic molecularly imprinted polymer into the solution to be detected; after shaking for 20-120 minutes, magnetically separating the magnetic molecularly imprinted polymer; (3) eluting the pyrimethamine in the magnetic molecularly imprinted polymer with an eluting solvent with a certain volume, and collecting an eluent; and (4) determining the concentration of the pyrimethamine in the eluent by utilizing HPLC/UV (High Performance Liquid Chromatography/Ultraviolet), wherein the magnetic molecularly imprinted polymer is prepared by taking the pyrimethamine as a template and amobarbital as a functional monomer. The magnetic molecularly imprinted polymer provided by the invention forms an intermolecular acting matrix through a multiple-hydrogen-bond effect between a template molecule and the functional monomer, so that the effect between the template molecule and the functional monomer is enhanced, and the affinity and selectivity on the pyrimethamine are improved.

Description

technical field [0001] The invention relates to the fields of organic chemistry and analytical chemistry, in particular to a separation and detection method for pyrimethamine. Background technique [0002] Pyrimethamine (PYR) is currently a widely used antimalarial drug, mainly used for the prevention of malaria. It can inhibit the activity of dihydrofolate reductase in parasites, thereby interfering with the normal metabolism of folic acid in Plasmodium. Protozoan erythrocytes are effective in the early stage and are often used as etiological preventive drugs. It can also inhibit the development of malaria parasites in mosquitoes, so it can block transmission. It is clinically used for the prevention of malaria and anti-relapse treatment of telogen. Malaria is estimated to kill 500 million clinical cases every year, and it is one of the diseases that kill the most people except AIDS and tuberculosis infectious diseases, which has attracted the attention of the Internation...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 梁勇
Owner SOUTH CHINA NORMAL UNIVERSITY
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