Separation and detection method of pyrimethamine

Abstract

The invention discloses a separation and detection method of pyrimethamine. The separation and detection method comprises the following steps: (1) adding acetonitrile into a sample; after shaking and uniformly mixing, centrifuging; taking supernatant and blowing with nitrogen gas; dissolving residues with a solvent to obtain a solution to be detected; (2) adding a magnetic molecularly imprinted polymer into the solution to be detected; after shaking for 20-120 minutes, magnetically separating the magnetic molecularly imprinted polymer; (3) eluting the pyrimethamine in the magnetic molecularly imprinted polymer with an eluting solvent with a certain volume, and collecting an eluent; and (4) determining the concentration of the pyrimethamine in the eluent by utilizing HPLC / UV (High Performance Liquid Chromatography / Ultraviolet), wherein the magnetic molecularly imprinted polymer is prepared by taking the pyrimethamine as a template and amobarbital as a functional monomer. The magnetic molecularly imprinted polymer provided by the invention forms an intermolecular acting matrix through a multiple-hydrogen-bond effect between a template molecule and the functional monomer, so that the effect between the template molecule and the functional monomer is enhanced, and the affinity and selectivity on the pyrimethamine are improved.

Description

technical field [0001] The invention relates to the fields of organic chemistry and analytical chemistry, in particular to a separation and detection method for pyrimethamine. Background technique [0002] Pyrimethamine (PYR) is currently a widely used antimalarial drug, mainly used for the prevention of malaria. It can inhibit the activity of dihydrofolate reductase in parasites, thereby interfering with the normal metabolism of folic acid in Plasmodium. Protozoan erythrocytes are effective in the early stage and are often used as etiological preventive drugs. It can also inhibit the development of malaria parasites in mosquitoes, so it can block transmission. It is clinically used for the prevention of malaria and anti-relapse treatment of telogen. Malaria is estimated to kill 500 million clinical cases every year, and it is one of the diseases that kill the most people except AIDS and tuberculosis infectious diseases, which has attracted the attention of the Internation...

AI Technical Summary

Problems solved by technology

[0006] However, due to the weak hydrogen bond force, only 1-2 hydrogen bonds are used to interact in general molecular imprinting techniques, and the adsorption capacity for target substances is limited.

Therefore, there are fewer binding sites between the template molecule and the functional monomer, and the selectivity to the template molecule is weak, so it cannot achieve effective selective adsorption on the template molecule.

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