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Method for preparing mannose through biological process

A mannose, biological method technology, applied in the biological field

Inactive Publication Date: 2016-07-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the catalytic production of mannose using a coenzyme regeneration system using glucose or fructose as raw materials

Method used

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  • Method for preparing mannose through biological process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In the water system, the final mass concentration of glucose is 5%, the final mass concentration of sodium formate is 0.5%, nicotinamide adenine dinucleotide (NAD + ) is 100mM, the final concentration of reduced nicotinamide adenine dinucleotide phosphate (NADPH) is 100mM, the reaction pH is regulated by formic acid, the reaction pH is 7.0, and the reaction temperature is 30 degrees. Glucose isomerization from Streptomycesmurinus The amount of enzyme is 350U / L, the amount of NADPH-dependent mannitol dehydrogenase derived from Agaricusbisporus is 1000U / L, and the amount of NADP derived from Burkholderiastabilis + The amount of dependent formate dehydrogenase is 1000U / L, the amount of NADH oxidase derived from Streptococcuspyogenes is 1000U / L, and the amount of NAD derived from Apiumgraveolens + The amount of dependent mannitol dehydrogenase is 1000U / L, the reaction is 12h, and the concentration of mannose in the reaction system is 3.5%.

Embodiment 2

[0025] The concentration of fructose is 5%, the concentration of sodium formate is 0.5%, the concentration of nicotinamide adenine dinucleotide (NAD + ) concentration is 100mM, the concentration of reduced nicotinamide adenine dinucleotide phosphate (NADPH) is 100mM, the reaction pH is regulated by formic acid, the reaction pH is 7.0, and the reaction temperature is 30 degrees. The NADP-dependent mannitol derived from Agaricusbisporus Hydrogenase 1000U / L, NAD(P) from Burkholderiastabilis + Dependent formate dehydrogenase 1000U / L, NADH oxidase 1000U / L from Streptococcuspyogenes, NAD from Apiumgraveolens + The dependent mannitol dehydrogenase was 1000U / L, reacted for 12h, and the mannose concentration in the reaction system was 4%.

Embodiment 3

[0027] Glucose concentration was 5%, fructose concentration was 5%, sodium formate concentration was 1%, nicotinamide adenine dinucleotide (NAD + ) concentration is 100mM, the concentration of reduced nicotinamide adenine dinucleotide phosphate (NADPH) is 100mM, the reaction pH is regulated by formic acid, the reaction pH is 7.0, the reaction temperature is 30 degrees, and the glucose isomerase from Streptomycesmurinus is 350U / L , NADP-dependent mannitol dehydrogenase from Agaricusbisporus 1000U / L, NAD from Burkholderiastabilis(P) + Dependent formate dehydrogenase 1000U / L, NADH oxidase 1000U / L from Streptococcuspyogenes, NAD from Apiumgraveolens + The dependent mannitol dehydrogenase was 1000U / L, reacted for 12h, and the mannose concentration in the reaction system was 7%.

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Abstract

The invention discloses a method for preparing mannose through a biological process. According to the method, one or two of sodium formate and calcium formate, and one or two of glucose and fructose are taken as the raw materials, under the condition that glucose isomerase is available or not available, a biological catalysis system composed of an NAD (P)H-dependent mannitol dehydrogenase, an NAD(P)<+>-dependent formate dehydrogenase, an NAD<+>-dependent mannitol dehydrogenase, an NADH oxidase and a coenzyme is adopted for catalyzing, so that the mannose is prepared. The method for preparing the mannose provided by the invention has the characteristics that the raw materials are cheap, the reaction selectivity is high, etc, and the method belongs to a novel preparation reaction route of the mannose.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a production method of mannose. Background technique [0002] Mannose, an epimer of glucose, has the functions of regulating immunity, resisting the infection of bacteria and viruses, and promoting the normal synthesis of glycoproteins in the body. In recent years, mannose has been widely used in the fields of functional food, feed additive and medicine, and has been developed into mannose functional food, mannose feed additive, therapeutic drugs for urinary tract inflammation and cystitis, and glycoprotein syndrome therapeutic drugs for sugar deficiency And so on a series of products. [0003] Mannose can be separated and prepared from some lignocellulose or glucomannan hydrolyzates containing mannose components. For example, people such as Ge Mingjuan have invented a kind of technology that takes konjac as raw material to prepare mannose by biodegradation method, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/24C12P19/02
Inventor 曹海龙尹恒李曙光王文霞陈玮
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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