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Haemadipsa sylvestris analgesic peptide mh2620 and gene and application thereof

A leech and forest technology, applied in the field of biomedicine, can solve the problems of structure and function that have not been reported.

Inactive Publication Date: 2016-07-06
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although leech analgesia is widely used clinically, there is no report on the structure and function of the material basis of its action

Method used

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  • Haemadipsa sylvestris analgesic peptide mh2620 and gene and application thereof
  • Haemadipsa sylvestris analgesic peptide mh2620 and gene and application thereof

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Embodiment Construction

[0023] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0024] Forest leech analgesic peptide gene clone:

[0025] I. Extraction of total RNA from forest leeches salivary glands:

[0026] A. The living forest leech was cleaned with water, put into liquid nitrogen and quick-frozen for 4 hours, took the salivary gland tissue, weighed, got 300mg tissue, added 10m1 total RNA extraction buffer (Trizol solution, product of GIBCOBRL company in the United States), and put it in 20m1 glass Homogenize in a homogenizer for 30 minutes.

[0027] B. Add an equal volume of phenol / chloroform solution, vortex and mix well, let stand at room temperature for 10 minutes, centrifuge at 12000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0028] C. Add an equal volume of isopropanol to the supernatant, place at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minut...

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Abstract

The invention relates to haemadipsa sylvestris analgesic peptide mh2620 and a gene and application thereof and belongs to the technical field of biomedicine.The haemadipsa sylvestris analgesic peptide mh2620 is single-link polypeptide subjected to haemadipsa sylvestris analgesic peptide gene coding, the molecular weight is 2620.13 Dalton, and the isoelectric point is 5.26.The haemadipsa sylvestris analgesic peptide mh2620 is composed of an amino acid sequence shown as SEQ ID NO: 1.The gene for encoding the haemadipsa sylvestris analgesic peptide mh2620 is composed of a nucleotide sequence shown as SEQ ID NO: 2.The haemadipsa sylvestris analgesic peptide mh2620 is applied to preparation of a sodium ion channel inhibitor and an analgesic drug.The haemadipsa sylvestris analgesic peptide mh2620 has the advantages of being capable of inhibiting a sodium ion channel, having a remarkable analgesic effect, and being simple in structure, convenient to synthesize artificially, high in analgesic activity and capable of being applied to preparation of the sodium ion channel inhibitor and the analgesic drug.

Description

Technical field: [0001] The invention provides Haemadipsasylvestris analgesic peptide mh2620 and its gene and application, belonging to the technical field of biomedicine. Background technique: [0002] Leech is a kind of traditional Chinese medicine in my country, which was described in "Shen Nong's Materia Medica" more than 2,000 years ago. "Compendium of Materia Medica" said: "Salty to remove blood, bitterness is better than blood. The salty and bitterness of leeches is used to remove blood accumulation, and it is the liver meridian blood to divide the medicine, so it can pass the liver meridian and gather blood." Chinese medicine believes that leeches are a traditional broken medicine. Blood medicine has the effects of dispelling blood stasis, dredging meridians, and benefiting water channels. It is mainly used to treat blood stasis amenorrhea, stroke hemiplegia, bruises and other diseases. [0003] Forest leech (Haemadipsasylvestris), also known as leeches. Hirudoclas...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12A61K38/17A61P29/00
CPCC07K14/43504A61K38/00
Inventor 赖仞龙承波吕秋敏容明强
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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