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Extracting, separating and preparing method of four sesquiterpene type active components in Tibet inula root

A technology of sesquiterpenoids and active ingredients, which is applied in the field of extraction, separation and preparation of four active ingredients of sesquiterpenoids in Akira sinensis, can solve the problems that have not yet been extracted from active ingredients in Akira sinensis, and achieve easy automatic control , Low production cost, realize the effect of automatic control

Inactive Publication Date: 2016-07-06
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the subcritical water extraction of active components in Akiras chinensis

Method used

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  • Extracting, separating and preparing method of four sesquiterpene type active components in Tibet inula root
  • Extracting, separating and preparing method of four sesquiterpene type active components in Tibet inula root
  • Extracting, separating and preparing method of four sesquiterpene type active components in Tibet inula root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for extracting, separating and preparing four sesquiterpene active components in chrysanthemum, comprising the following steps:

[0034] (1) Pulverize the root of Akiras sinensis to 20 orders to obtain the powder of Akissica sinensis;

[0035] (2) put the Akira sinensis powder obtained in step (1) into the extraction kettle of a subcritical water extraction device, and use deionized water as the extractant for continuous flow extraction, the flow rate of water is controlled at 1mL / min, and the pressure is controlled at 0.5MPa. The temperature is controlled at 120°C, the extraction time is 30 minutes, and the final extract is obtained;

[0036] (3) Put the extract obtained in step (2) into the centrifuge tube of the centrifuge, after centrifuging at a speed of 2000r / min for 5min, remove the centrifuge tube to obtain the supernatant;

[0037] (4) Concentrate and recover the supernatant obtained in step (3) on a rotary evaporator at a temperature of 55° C. and ...

Embodiment 2

[0047] The method for extracting, separating and preparing four sesquiterpene active components in chrysanthemum, comprising the following steps:

[0048] (1) Pulverize the root of Akiras sinensis to 60 orders to obtain the powder of Akissica sinensis;

[0049] (2) Put the Akira sinensis powder obtained in step (1) into the extraction kettle of a subcritical water extraction device, and use distilled water as the extractant for continuous flow extraction, the flow rate of water is controlled at 20mL / min, and the pressure is controlled between 3.0MPa , the temperature is controlled between 250°C, the extraction time is 90min, and the final extract is obtained;

[0050] (3) Put the extract obtained in step (2) into the centrifuge tube of the centrifuge, after centrifuging at a speed of 6000r / min for 15min, remove the centrifuge tube to obtain the supernatant;

[0051] (4) Concentrate and recover the supernatant obtained in step (3) on a rotary evaporator at a temperature of 80°...

Embodiment 3

[0057] The method for extracting, separating and preparing four sesquiterpene active components in chrysanthemum, comprising the following steps:

[0058] (1) Pulverize the root of Akiras sinensis to 40 orders to obtain the powder of Akissica sinensis;

[0059] (2) Put the Akira sinensis powder obtained in step (1) into the extraction kettle of a subcritical water extraction device, and use high-purity water as the extractant for continuous flow extraction, the flow rate of water is controlled at 10mL / min, and the pressure is controlled between 0.4MPa , the temperature is controlled between 185°C, the extraction time is 60min, and the final extract is obtained;

[0060] (3) Put the extract obtained in step (2) into the centrifuge tube of the centrifuge, after centrifuging at a speed of 4000r / min for 10min, remove the centrifuge tube to obtain the supernatant;

[0061] (4) Concentrate and recover the supernatant obtained in step (3) on a rotary evaporator at a temperature of 6...

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Abstract

The invention relates to an extracting, separating and preparing method of four sesquiterpene type active components in Tibet inula root.The method includes the following steps of smashing Tibet inula root, extracting an extraction solution through supercritical water, conducting concentration, extracting an obtained concentrated solution through ethyl acetate to obtain an ethyl acetate extraction solution, conducting pressure reduced evaporation to dryness on the ethyl acetate extraction solution till the weight is unchanged to obtain an extract, dissolving the extract through methyl alcohol, conducting isocratic elution through preparation type high-efficiency liquid phase chromatography to obtain igalan, isoalantolactone, alantolactone and Dugesialactone eluant, and conducting pressure reduced evaporation to dryness on the eluant till the weight is unchanged to obtain light yellow powdery igalan, isoalantolactone, alantolactone and Dugesialactone.The method is simple, convenient and rapid to implement, and the purity of all obtained monomers reaches 98% or above.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of active ingredients of natural products, and in particular relates to a preparation method for extraction, separation and preparation of four active ingredients of sesquiterpenoids in Akira saponifolium. technical background [0002] Inularacemosa Hook.f is a plant of the genus Inulae in the family Compositae. It is mainly distributed in Sichuan, Qinghai, Gansu, Tibet and other provinces in China. The root is used as medicine. It is a commonly used medicinal material in Tibetan and Mongolian medicine. In the "Standards of Tibetan Medicine" compiled by the health bureaus of Tibet and Qinghai, there are 290 kinds of prescription preparations in total, among which there are 75 kinds of preparations using Tibetan woody fragrance, accounting for about 26% of Tibetan medicine prescriptions, which is a very high usage rate among Tibetan medicinal materials. One of the medicinal mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/92
CPCC07D307/92Y02P20/54
Inventor 迟晓峰胡风祖董琦肖远灿陈世龙
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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