Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of microbial oil

A technology of microbial oil and mixed oil, applied in fermentation and other directions, can solve the problems of high surface oil content, poor emulsification performance of microbial oil, and inability to form encapsulation of microbial oil, so as to extend shelf life, reduce surface oil content, and improve resistance to The effect of oxidative power

Active Publication Date: 2019-05-10
CABIO BIOTECH WUHAN CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial oil has the following defects: since the triglyceride has no hydrophilic group and has no emulsifying properties, the microbial oil has poor emulsifying properties
In the subsequent microcapsule production process, microbial oil cannot form a good embedding, and the final microcapsule product has a high surface oil content, which is not conducive to further subsequent production and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Taking Mortierella alpina as the starting strain, the production and application of the microbial oil containing arachidonic acid of the present invention are described in detail.

[0020] 1. Fermentation

[0021] Prepare a medium solution with a glucose content of 0.03g / mL and a yeast powder content of 0.02g / mL in a 500ml shaker flask. Multiple bottles can be prepared. After sterilization, insert appropriate amount of Mortierella alpina mycelia and spores, and place at 28°C Constant temperature shaker, 150rpm, after 2 days, merge the shaker flasks, and transfer them into a 1m medium solution containing 0.03g / mL glucose content and 0.02g / mL yeast powder content that has been sterilized. 3 In the fermenter (first-class seed tank), sterile air is continuously introduced to keep the culture temperature at 28±2°C. After 2 days of cultivation in the first-level seed tank, transfer all the culture solution into a 10mL medium solution with a glucose content of 0.03g / mL and a ...

Embodiment 2

[0080] Taking Schizochytrium as the starting strain, the production and application of the microbial oil containing docosahexaenoic acid of the present invention are described in detail.

[0081] 1. Fermentation

[0082] Prepare a culture medium solution with a glucose content of 0.04g / mL and a yeast extract content of 0.02g / mL in a 1000ml shaker flask. Multiple bottles can be prepared. After sterilization, add an appropriate amount of refrigerated Schizochytrium bacteria solution and place it at a constant temperature of 28°C for shaking Bed, 180rpm for activation. After 2 days, they were inserted into the secondary expansion shaker flasks for cultivation. After 2 days, the shaker flasks were merged and transferred into a sterilized 1m medium containing 5wt% glucose and 2wt% yeast extract. 3 In the fermenter (first-level seed tank), sterile air was continuously introduced to keep the culture temperature at 29±1°C. After 2 days of cultivation in the primary seed tank, transf...

Embodiment 3

[0110] Taking Pythium ultima as the starting strain, the production and application of microbial oil containing eicosapentaenoic acid are described in detail.

[0111] 1. Fermentation

[0112] Prepare a medium solution with a sucrose content of 0.05g / mL and a yeast powder content of 0.005g / mL in a 1000ml shaker flask. Multiple bottles can be prepared. After sterilization, add an appropriate amount of Pythium ultima and place it on a constant temperature shaker at 28°C. Activation was performed at 180 rpm. After 2 days, insert the secondary expansion flask for culture, combine the shake flasks after 2 days, and transfer into the sterilized 1m 3 In the fermenter (first-class seed tank), sterile air was continuously introduced to keep the culture temperature at 28±1°C. After 2 days of cultivation in the primary seed tank, transfer all the culture solution into a sterilized 10mL container with 0.05g / mL sucrose and 0.005g / mL yeast powder. 3 In the fermenter (secondary seed tank)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to microbe oil and a preparation method thereof. In the microbe oil, content of polyunsaturated fatty acids (PUFA) is more than 30wt%, content of triglyceride is less than 90wt%, and content of diglyceride is not less than 5wt% and not more than 20wt%. The preparation method of the microbe oil comprises the following steps of: obtaining fermentation broth rich in PUFA microbe oil through fermentation by virtue of oil-producing microorganisms; collecting thalli rich in the PUFA microbe oil, extracting and filtering, so that mixed oil is obtained; and adding lipase and water into the mixed oil for carrying out enzymolysis, purifying, so that the microbe oil is obtained, or adding mixture containing diglyceride into the mixed oil, uniformly mixing, and removing solvent, so that the microbe oil is obtained. The microbe oil contains right amount of diglyceride, so that the microbe oil can conveniently form stable emulsified liquid. In a process of preparing a microcapsule, the microbe oil can be better embedded, further surface oil content of the microcapsule can be reduced, antioxidant capacity of the microcapsule is improved, shelf life of the microcapsule can be moderately prolonged, and follow-up further production and utilization can be benefited.

Description

technical field [0001] The invention relates to a preparation method of microbial oil. Background technique [0002] Polyunsaturated fatty acid (PUFA) refers to fatty acids containing two or more double bonds, generally containing 18 to 22 carbon atoms. Industrialized PUFAs are mostly produced by single-cell microorganisms such as fungi and algae, so they are also called microbial oils. [0003] Polyunsaturated fatty acids are mainly divided into two series of omega-3 and omega-6 because of their structural characteristics. The omega-3 series includes octadecatrienoic acid (commonly known as α-linolenic acid, ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). The omega-6 series includes octadecadienoic acid (commonly known as linoleic acid, LA), stearidonic acid (commonly known as gamma-linolenic acid, GLA), and eicosadonic acid (commonly known as arachidonic acid). Polyunsaturated fatty acids are the main components of human cell membrane phospholipids, w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
CPCC12P7/6418C12P7/6427C12P7/6463
Inventor 汪志明周强陆姝欢肖敏田勇李翔宇
Owner CABIO BIOTECH WUHAN CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com