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Method for extracting tyrosinase inhibitor from common camellia bee pollen

A technology of tyrosinase and Camellia bee, which is applied in the field of preparation of tyrosinase inhibitors, can solve the problems of low added value, low price, and insufficient development and utilization of bee pollen resources, so as to reduce damage and shorten the cycle , Improve the effect of added value and economic benefits

Active Publication Date: 2016-06-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is rich in bee pollen resources, but the bee pollen currently on the market is mainly crude products that are simply sterilized, with low added value. Compared with internationally famous bee products, the price is lower, and bee pollen resources have not been fully developed and utilized.

Method used

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  • Method for extracting tyrosinase inhibitor from common camellia bee pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Grinding the dried camellia bee pollen, adding 10 times the weight of petroleum ether, ultrasonication for 1 hour, degreasing 3 times, suction filtration to obtain the filter residue, drying the filter residue, and removing the residual petroleum ether;

[0022] (2) Add 80% ethanol aqueous solution as extractant to the degreased camellia bee pollen, the ratio of solid to liquid is 1g:30ml, ultrasonic extraction is carried out under the condition of 50°C, the extraction time is 35min, the ultrasonic power is 100W, and the extraction times are 3 times, after extraction, suction filtration, rotary evaporation and concentration of the extract, and vacuum freeze-drying to obtain the alcoholic extract of camellia bee pollen;

[0023] (3) Add distilled water to the alcohol extract of camellia bee pollen by the ratio of 1g:10ml with the alcohol extract of camellia bee pollen and distilled water obtained above, and ultrasonically disperse the alcohol extract of camellia bee p...

Embodiment 2

[0026] (1) Grinding the dried camellia bee pollen, adding 5 times the weight of petroleum ether, ultrasonication for 2 hours, degreasing 3 times, suction filtration to obtain the filter residue, drying the filter residue, and removing the residual petroleum ether;

[0027] (2) Add 70% ethanol aqueous solution to the degreased camellia bee pollen as an extractant, the ratio of solid to liquid is 1g:40ml, ultrasonic extraction is carried out at 40°C, the extraction time is 40min, the ultrasonic power is 200W, and the extraction times are 3 times, after extraction, suction filtration, rotary evaporation and concentration of the extract, and vacuum freeze-drying to obtain the alcoholic extract of camellia bee pollen;

[0028] (3) With the alcohol extract of camellia bee pollen and distilled water obtained above as a ratio of 1g: 6ml, add distilled water to the alcohol extract of camellia bee pollen, ultrasonically disperse the alcohol extract of camellia bee pollen into distilled w...

Embodiment 3

[0031] (1) Grinding the dried camellia bee pollen, adding 20 times the weight of petroleum ether, ultrasonic degreasing once, suction filtration to obtain the filter residue, drying the filter residue, and removing the residual petroleum ether;

[0032] (2) Add 90% ethanol aqueous solution to the degreased camellia bee pollen as an extractant, the ratio of solid to liquid is 1g: 20ml, carry out ultrasonic extraction at 45°C, the extraction time is 30min, the ultrasonic power is 100W, and the extraction times are 2 After extraction, suction filtration is performed, the extract is concentrated by rotary evaporation, and vacuum freeze-dried to obtain the alcoholic extract of camellia bee pollen;

[0033] (3) With the alcohol extract of Camellia bee pollen and distilled water obtained above as a ratio of 1g: 8ml, add distilled water to the alcohol extract of Camellia bee pollen, dissolve it by ultrasonic and centrifuge, and separate the filtrate;

[0034] (4) Adsorb the AmberliteX...

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Abstract

The invention discloses a method for extracting a tyrosinase inhibitor from common camellia bee pollen. The method comprises the steps that the common camellia bee pollen are firstly smashed and degreased, then ultrasonic extraction is performed by adopting ethanol, and the obtained alcohol extract is purified by using macroporous resin. The tyrosinase inhibiting effect of the common camellia bee pollen extract obtained by adopting the method is improved obviously, and the optimal half inhibition ratio can reach 0.35 mg / ml. Therefore, the method can be widely used for preparation of skin-whitening and health-care products and skin-care products for preventing and treating human melanin pigmentation and food additives for inhibiting enzymatic browning of foods.

Description

technical field [0001] The invention relates to a preparation method of a tyrosinase inhibitor, in particular to a method for extracting a tyrosinase inhibitor from camellia bee pollen. Background technique [0002] Tyrosinase is the key rate-limiting enzyme in the biosynthesis of skin melanin. This enzyme catalyzes the formation of dopa from tyrosine, and then generates dopaquinone with dopa as the substrate. Dopaquinone reacts under non-enzymatic conditions to finally Synthetic melanin. The activity of tyrosinase is closely related to the synthesis of melanin. If the activity of tyrosinase is too high, it will easily lead to the formation of various spots and even melanoma. Tyrosinase is also the main enzyme for enzymatic browning of fruits and vegetables. During the process of storage, transportation and freshness of fruits and vegetables, browning occurs under the action of oxygen and tyrosinase, resulting in sensory and quality degradation, affecting the transportation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61K35/64A61K8/97A61K8/98A61Q19/02A61P17/00A23L5/41A23L33/10
CPCA23V2002/00A61K8/97A61K8/987A61K35/63A61K36/82A61K2236/333A61K2236/39A61K2236/53A61K2236/55A61Q19/02A23V2200/048A23V2200/318A23V2250/21A23V2300/14
Inventor 鲁群雷润梅刘睿杜鸣阳苏佳
Owner HUAZHONG AGRI UNIV
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