Method for preparing polysaccharide beverage by fermenting apples with lucid ganoderma mycelium liquid
A technology of Ganoderma lucidum mycelium and liquid fermentation, which is applied in the fields of fermentation engineering and food engineering to achieve the effect of improving nutritional value and activity
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Embodiment 1
[0022] Example 1: Production of apple fermented polysaccharide beverage according to the following steps
[0023] (1) Preparation of Ganoderma lucidum mycelium liquid strain: Inoculate the Ganoderma lucidum slant strains into sterilized PDY medium at 30°C, 150r / min, and cultivate for 4 days;
[0024] (2) Preparation of apple juice fermentation medium :Select Shandong Red Fuji apples, obtain apple juice after washing, dicing, beating and filtering, then add 0.5% edible starch, 0.5% edible sugar and 0.5% milk powder according to the mass ratio, dissolve, autoclave and sterilize to prepare apples Juice fermentation medium;
[0025] (3) Liquid fermentation of Ganoderma lucidum mycelium : Inoculate the Ganoderma lucidum mycelium liquid strain into the apple juice fermentation medium at an inoculum of 10% by volume for fermentation; 200r / min, 28℃, aeration VVM of 1:1, fermentation for 7 days; fermentation broth after fermentation The polysaccharide content reaches about 2700mg / L;
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Embodiment 2
[0027] Example 2: Production of composite polysaccharide beverage according to the following steps
[0028] (1) Preparation of Ganoderma lucidum mycelium liquid strain: Inoculate the Ganoderma lucidum slant strain into sterilized PDY medium, culture at 28°C, 150r / min, for 5 days;
[0029] (2) Preparation of apple juice fermentation medium :Choose Luochuan apples from Shaanxi, obtain apple juice after washing, dicing, beating, and filtering. Then add 0.1% edible starch, 2% edible sugar and 1% milk powder according to the mass ratio, dissolve, autoclave and sterilize the apples. Juice fermentation medium;
[0030] (3) Liquid fermentation of Ganoderma lucidum mycelium : Inoculate the Ganoderma lucidum mycelium liquid strain into the apple juice fermentation medium at an inoculum of 10% by volume for fermentation; 350r / min, 32℃, ventilation VVM of 1:1.5, fermentation for 5 days; fermentation broth after fermentation The polysaccharide content reaches about 2800mg / L;
[0031] (4) Com...
Embodiment 3
[0032] Example 3: Production of composite polysaccharide beverage according to the following steps
[0033] (1) Preparation of Ganoderma lucidum mycelium liquid strain: Inoculate the Ganoderma lucidum slant strains into sterilized PDY medium at 30°C, 150r / min, and cultivate for 4 days;
[0034] (2) Preparation of apple juice fermentation medium :Select Henan Lingbao apples, obtain apple juice by washing, dicing, beating and filtering, then add 1% edible starch, 0.2% edible sugar and 0.2% milk powder according to the mass ratio, after dissolving, autoclave sterilization to prepare apples Juice fermentation medium;
[0035] (3) Liquid fermentation of Ganoderma lucidum mycelium : Inoculate the Ganoderma lucidum mycelium liquid strain into the apple juice fermentation medium at an inoculum of 10% by volume for fermentation; 150r / min, 25℃, ventilation VVM of 1:0.5, fermentation for 7 days; fermentation broth after fermentation The polysaccharide content reaches about 2500mg / L;
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