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Method for directly inducing regenerated plants in vitro by means of mature sapium sebiferum endosperm

A technology for regenerating tallow tree and Chinese tallow tree, which is applied in the field of direct regeneration of tallow tallow mature endosperm in vitro. It can solve the problems of difficulty in satisfying Chinese tallow tallow polyploid breeding, low regeneration efficiency, cumbersome regeneration process, etc., and achieve good disinfection effect and fast regeneration speed , The effect of simple regeneration process

Active Publication Date: 2016-06-29
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are also reports on the in vitro regeneration of Chinese tallow tree using the endosperm of Chinese tallow tree as explants, but they mainly focus on the research on plant regeneration through the induction and differentiation of callus tissue, and there are problems such as low regeneration efficiency and cumbersome regeneration process. , it is difficult to meet the needs of Chinese tallow tree polyploid breeding

Method used

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  • Method for directly inducing regenerated plants in vitro by means of mature sapium sebiferum endosperm
  • Method for directly inducing regenerated plants in vitro by means of mature sapium sebiferum endosperm
  • Method for directly inducing regenerated plants in vitro by means of mature sapium sebiferum endosperm

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Embodiment 1

[0033] A method for directly regenerating plants from tallow mature endosperm in vitro, specifically as follows:

[0034] (1) Take the mature seeds of Chinese tallow tree and cut off the seed shell, and carry out surface disinfection in the aseptic operating table; surface disinfection refers to disinfection with 75% absolute ethanol for 30 seconds, and then disinfection with 20% sodium hypochlorite solution for 8 minutes, and rinse with sterile water 3 times, and then subjected to 600W ultrasonic treatment for 7 minutes, and kept shaking during the disinfection of the seed surface.

[0035] (2) The sterilized seeds in step (1) were inoculated in MS medium supplemented with 2.0 mg / L 6-BA, 0.2 mg / LTDZ, 30 g / L sucrose and 7.0 g / L agar for endosperm initiation culture.

[0036] (3) After 6-9 days of light culture, the endosperm was stripped from the germinating Chinese tallow tree seeds, and inoculated in MS culture supplemented with 3.0 mg / L 6-BA, 0.05 mg / LTDZ, 30 g / L sucrose an...

Embodiment 2

[0040] A method for directly regenerating plants from tallow mature endosperm in vitro, specifically as follows:

[0041] (1) Take mature tallow tree seeds and cut off the seed husks, and carry out surface disinfection in an aseptic operating table; surface disinfection refers to disinfection with 75% absolute ethanol for 40 seconds, then disinfection with 20% sodium hypochlorite solution for 10 minutes, and rinse with sterile water 4 times, and then subjected to 600W ultrasonic treatment for 10 minutes, and kept shaking during the surface disinfection of the seeds.

[0042] (2) Inoculate the sterilized seeds in step (1) into MS medium supplemented with 5.0 mg / L 6-BA, 0.5 mg / LTDZ, 30 g / L sucrose and 7.0 g / L agar for endosperm priming culture.

[0043] (3) After 6-9 days of light culture, the endosperm was stripped from the germinating tallow tree seeds ( figure 1 ), inoculated on the MS medium that added 4.5mg / L6-BA, 0.2mg / LTDZ, 30g / L sucrose and 7.0g / L agar to induce adventi...

Embodiment 3

[0047]A method for directly regenerating plants from tallow mature endosperm in vitro, specifically as follows:

[0048] (1) Take the mature seeds of Chinese tallow tree and cut off the seed shell, and carry out surface disinfection in the aseptic operating table; surface disinfection refers to disinfecting with 75% absolute ethanol in the aseptic operating table for 45 seconds, and then disinfecting with 20% sodium hypochlorite solution for 12 minutes , rinsed with sterile water 5 times, and then subjected to 600W ultrasonic treatment for 8 minutes, and kept shaking during the surface disinfection of the seeds.

[0049] (2) Inoculate the sterilized seeds in step (1) in MS medium supplemented with 8.0 mg / L 6-BA, 1.0 mg / LTDZ, 30 g / L sucrose and 7.0 g / L agar for endosperm priming culture.

[0050] (3) After 6-9 days of light culture, the endosperm was stripped from the germinating tallow tree seeds, and inoculated in MS culture supplemented with 6.0 mg / L 6-BA, 0.5 mg / LTDZ, 30 g / ...

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Abstract

The invention discloses a method for directly inducing regenerated plants in vitro by means of mature sapium sebiferum endosperm. The method comprises the steps of 1, shearing seed cases off mature sapium sebiferum seeds, conducting surface sterilization, then inoculating an initial medium containing a growth regulator with the seeds, and conducting illumination for endosperm initiation culture; 2, peeling endosperm off the sapium sebiferum seeds, inoculating an adventitious bud induction medium with the seeds, and conducting illumination for adventitious bud induction, so that light green clumpy adventitious buds are obtained; 3, dicing the clumpy adventitious buds, and transplanting the clumpy adventitious buds into a multiplication and elongation medium for adventitious bud multiplication and elongation; 4, transplanting the clumpy adventitious buds into a rooting medium for rooting, so that robust regenerated sapium sebiferum plants are obtained. The method has the advantages that the regeneration process is simple, induction of callus is omitted, and clumpy adventitious buds can be directly induced on the epidermis around endosperm.

Description

technical field [0001] The invention relates to a method for directly regenerating plants of tallow mature endosperm in vitro, and belongs to the field of biotechnology. Background technique [0002] Sapium sebiferum (Sapium sebiferum) is a perennial woody plant of the Euphorbiaceae Sapium genus, widely distributed in tropical and subtropical regions of my country, and is a unique economic tree species in my country. Chinese tallow tree has a neat crown, beautiful leaf shape, and bright red, purple or bright yellow leaves in autumn. It integrates appearance, leaves and fruit, and has very high ornamental value. It can be used as garden greening and street trees. In addition, Chinese tallow tree has strong adaptability, low requirements on soil quality, tolerance to drought, waterlogging, barrenness, suitable for marginal land planting, and various planting modes, which is easy to realize large-scale large-scale production. Therefore, Chinese tallow tree has attracted widesp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 侯金艳吴丽芳赵华
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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