Molecular breeding method based on single nucleotide polymorphism of cattle Angpt18 genes

Abstract

The invention discloses a molecular breeding method based on the single nucleotide polymorphism of cattle Angpt18 genes.The molecular breeding method includes the steps that to-be-detected cattle whole genome DNA with the Angpt18 genes serves as a template, primer pairs P serve as primers, the cattle Angpt18 genes are subjected to PCR amplification, and PCR amplification products are obtained; the PCR amplification products are digested with restriction enzyme HinfI to obtain enzyme-digested product fragments, and the enzyme-digested product fragments are subjected to polyacrylamide gel electrophoresis; the polyacrylamide-gel-electrophoresis result shows that the 308th bits of the cattle Angpt18 genes have the single nucleotide polymorphism; meanwhile, the detecting result of the single nucleotide polymorphism of the cattle Angpt18 genes and the growth characteristics of a cattle are analyzed in a relevance mode, suitable genotypes are used for improving molecular breeding of the growth characteristics of the cattle, and cattle populations with the excellent genetic resources can be rapidly established.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a molecular breeding method based on the single nucleotide polymorphism of the cattle Angptl8 gene. Background technique [0002] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) refers to the polymorphism caused by the replacement of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Substitutions, insertions, deletions, and copy number changes in repetitive sequences. In diploid biological populations, SNPs can theoretically consist of 2, 3 or 4 alleles, but in practice SNPs with 3 or 4 alleles are very rare, so SNPs are usually called dialleles Gene molecular markers. According to the location of SNP distribution in the genome, SNP can be divided into gene coding region SNP (cSNP), gene peripheral SNP (pSNP) and intergenic SNPs (iSNP) and other types. Among them, cSNP has a greater impact and may affect the function of the p...

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Problems solved by technology

[0006] The most common detection methods for SNP polymorphism are single-strand conformational polymorphism technology and direct sequencing. higher

In addition, the research on the genetic variation of the Angptl8 gene in Chinese native yellow cattle is still blank

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