A kind of acquisition method of Mortierella Chinese strain

A technology for obtaining Trichosporium chinensis and an acquisition method, which is applied in the field of obtaining high-quality strains of Trichospora chinensis in China, can solve the problems of uneven quality of strains, long periods of time, and complicated breeding work.

Active Publication Date: 2018-02-02
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The traditional ways to obtain Mortierella chinensis strains include tissue isolation (insect body isolation, fruiting body part isolation), ascospore isolation and intrabasal isolation. The intrabasal isolation is rarely reported due to the complexity of the operation. Both tissue isolation and ascospore isolation can obtain more Mortierella sinensis strains, but the quality of the obtained strains varies, and a large amount of screening work is required in the later stage, and a small number of strains suitable for fermentation are selected for fermentation, or through various forms. Induction of strains to eliminate unfavorable factors that affect the yield due to the production of melanin during the fermentation process of the strain or the degeneration of the strain after a small number of passages, but this often requires a long cycle and complicated breeding work

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  • A kind of acquisition method of Mortierella Chinese strain
  • A kind of acquisition method of Mortierella Chinese strain
  • A kind of acquisition method of Mortierella Chinese strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the method of the present invention obtains high-quality Mortierella chinensis strain

[0028] 1. Collect the digestive juice of the bat moth larvae

[0029] (1) Take 50 infected 3rd instar bat moth larvae, and use absorbent cotton dipped in 75% alcohol solution to wipe the surface of the larvae in a sterile environment for disinfection.

[0030] (2) Take a piece of filter paper and spread it on the surface of the sterilized Petri dish, spray a small amount of sterile water on the filter paper to make the filter paper fully wet, full but not overflowing with water. Larvae were placed on filter paper, one worm per Petri dish to prevent biting. Under the conditions of temperature 8°C, relative humidity 65%, and no light, do 8h starvation treatment.

[0031] (3) Lay the larvae flat on the table, cover the larvae with a small piece of filter paper (length*width, 10*7cm) above the chest and abdomen, and expose the larvae’s head and chest 1-2 joints, and fix t...

Embodiment 2

[0035] Embodiment 2: Identification of Mortierella sinensis strains obtained

[0036] The colony morphology was observed, the state of conidia was observed microscopically, and the obtained Mortierella sinensis strain was amplified by ITS-specific PCR and sequenced.

[0037] (1) Colony morphology: the center of the colony protrudes, the inner edge is brown-gray to dark brown wrinkled, the outer edge is neatly covered with a thin layer of aerial hyphae, the colony is compact, and the black pigment on the back spreads to the surrounding, such as figure 2 shown.

[0038] (2) Microscopic observation of conidial state: the hyphae are thick and clear, the primary hyphae germinate and grow to form secondary hyphae, and the secondary hyphae branch again or not. Conidiophores are formed at the top of the secondary hyphae, and then the top of the postconidiophores is specialized, and gradually grows and expands to form a phialoid structure. The phialoid structure undergoes constriction ...

Embodiment 3

[0040] Embodiment 3: the method described in the present invention obtains high-quality Trichospora sinensis strains

[0041] 1. Collect the Digestive Fluid of Manta moth larvae

[0042] (1) Take 50 infected 4th instar bat moth larvae and wipe the surface of the insect body with absorbent cotton dipped in 75% alcohol solution in a sterile environment for disinfection.

[0043] (2) Take a piece of filter paper and lay it flat on the surface of the sterilized petri dish, and spray a small amount of sterile water on the filter paper, so that the filter paper is fully wetted, full but not overflowing with water. Larvae were placed on filter paper, one worm per petri dish to prevent biting. Do starvation treatment for 12h under the condition of temperature 10℃, relative humidity 70%, and no light.

[0044] (3) Lay the larva on the table, cover the top of the larva with a small piece of filter paper (length*width, 12*8cm), expose the head and 1-2 sections of the thorax, and fix th...

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Abstract

The invention relates to a method for acquiring a high quality Hirsutella sinensis strain. According to the method, a Hirsutella sinensis strain is acquired from a ghostmoth larva digestive tract (mainly intestinal juice) infected with Hirsutella sinensis bacterium. The method is simple to operate and has no damage to ghostmoth larvae, digestive juice (mainly intestinal juice) collected larvae can further grow normally, and can grow into Ophiocordyceps sinensis. Also, the obtained strain can directly serve as an initial strain of industrial fermentation without screening and induction, and the mycelium yield is high.

Description

technical field [0001] The invention belongs to the field of biological sciences, and in particular relates to a method for obtaining high-quality strains of Mortierella chinensis. Background technique [0002] Cordyceps sinensis [Ophiocordyceps sinensis (Berk.) G.H.Sung et al.] is a traditional traditional Chinese medicine in my country. Cordyceps sinensis due to technical and cost reasons, the output is limited, resulting in high prices of Cordyceps sinensis. The fermentation mycelium of Cordyceps sinensis is more and more favored because it has similar effects to those of Cordyceps sinensis in some respects. A good source of strains is the key restriction factor for fermentation. Years of research have confirmed that Chinese hairy sporophytes are the source of Cordyceps sinensis. Obtaining a high-quality strain of Mortierella chinensis will play a major role in promoting the fermentation industry of Mortierella chinensis. [0003] The traditional ways to obtain Mortierel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 李文佳吕延华李全平张宗耀
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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